* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download No Slide Title
Expanded genetic code wikipedia , lookup
RNA polymerase II holoenzyme wikipedia , lookup
RNA silencing wikipedia , lookup
Gene expression profiling wikipedia , lookup
X-inactivation wikipedia , lookup
Eukaryotic transcription wikipedia , lookup
Molecular cloning wikipedia , lookup
Epitranscriptome wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Genetic code wikipedia , lookup
Gene regulatory network wikipedia , lookup
Promoter (genetics) wikipedia , lookup
Non-coding RNA wikipedia , lookup
Community fingerprinting wikipedia , lookup
Genome evolution wikipedia , lookup
Genetic engineering wikipedia , lookup
Biosynthesis wikipedia , lookup
Non-coding DNA wikipedia , lookup
List of types of proteins wikipedia , lookup
Transcriptional regulation wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Endogenous retrovirus wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Gene expression wikipedia , lookup
Point mutation wikipedia , lookup
Molecular evolution wikipedia , lookup
GENETIC-CONCEPTS 1.Genome 2.Chromosome 3.Gene 4.DNA/RNA 5.Nucleic Acid 6.Protein 7.Amino Acid Genome Entirely of an organism’s heredity information Complete set of instructions for making an organism All of the hereditary information encoded in an organism’s DNA. Master blueprints for all enzymes, cellular structures & activities An organism‘s complete set of DNA All the DNA contained in the cell of an organism The collection of DNA that comprises an organism Prokaryotic Organism • Eubacteria and Archaea. Usually unicellular. • No internal membrane-bound compartments: DNA floats free in the cytoplasm. • 1 circular chromosome (plus optional plasmids, which are also circular) • reproduction usually asexual • sexual processes (mixing DNA from 2 individuals) occur, but with unequal contributions from the 2 partners • transcription and translation simultaneous Prokaryotic genomes Most have a single, doublestranded circular DNA molecule Usually without introns Since there is no nucleus, the DNA floats freely within the cell Proteins cause the DNA to coil tightly forming a nucleoid region Relatively high gene density Often indigenous plasmids are present Eukaryotic Organism • • • • Plants, animals, fungi, protistas. Often multicellular. DNA contained within a membrane-bound nucleus. linear chromosomes (usually more than 1) careful division of chromosomes in cell division: mitosis and meiosis • transcription separated from translation • sexual reproduction: 2 partners contribute equally to offspring • life cycle: alternation of haploid and diploid phases (i.e. 1 vs. 2 copies of each gene and chromosome) Eukaryotic genomes Genetic information is divided in the chromosome. The size of genomes is species dependent The difference in the size of genome is mainly due to a different number of identical sequence of various size arranged in sequence The gene for ribosomal RNAs occur as repetitive sequence and together with the genes for some transfer RNAs in several thousand of copies Structural genes are present in only a few copies, sometimes just single copy. Structural genes encoding for structurally and functionally related proteins often form a gene family The DNA in the genome is replicated during the interphase of mitosis Eukaryotic Genome Chromosome A DNA – histone protein thread, usually associated with RNA, occurring in the nucleus of a cell Chromosomes contain hundreds of genes encoded within their DNA Chromosome Logical Structure Locus Location of a gene/marker on the chromosome. Allele One variant form of a gene/marker at a particular locus. Locus1 Possible Alleles: A1,A2 Locus2 Possible Alleles: B1,B2,B3 10 Gene The material that controls which traits are expressed in an organism Genes come in pairs and offspring inherit one copy of each gene from each parent A section of DNA that codes for a trait Material of heredity Heredity The passing of traits from parent to offspring Allele The different forms of a trait that a gene may have. One form of a gene Traits Ways of looking, thinking, or being. Traits that are genetic are passed down through the genes from parents to offspring Recessive A trait that is covered over, or dominated, by another form of that trait and seems to disappear Hidden when the other copy of the gene contains the dominant allele. A recessive allele shows up only when there is no dominant allele present Shown with a lower-case letter (a) Dominant A trait that covers over, or dominates, another form of that trait Trait that always shows up, even when only one of the two alleles is in the dominant form Shown by a capital letter (A) Homozygous Both alleles [forms of the gene] are the same When offspring inherit two dominant genes, (one dominant gene from each parent) they are said to be homozygous dominant (AA) When offspring inherit two recessive genes, (one recessive gene from each parent) they are said to be homozygous recessive (aa) Heterozygous When alleles occur in different forms When offspring inherit one dominant gene and one recessive gene, they are said to be heterozygous (Aa) Since the dominant gene will be expressed, they are said to be heterozygous dominant (Aa) Genotype An organism's genetic makeup Genotype PHENOTYPE Outward physical appearance and behavior of an organism PHENOTYPE Genes Segment of DNA which can be transcribed and translated to amino acid Central Dogma of Biology DNA, RNA, and the Flow of Information Replication Transcription Translation Central Dogma (Modifications) (2)Ribozymes Transcription DNA RNA Translation Protein (1) Reverse transcription Replication (2)Self Replication (3)Self Replication DNA as Genetic Material DNA encodes all the information in the cell The composition of the DNA is the same in all cells within an organism • Variation among different cells is achieved by reading the DNA differently DNA contains four bases that encode all the information to make an organism’s life RIBO NUCLEIC ACID A polymer composed of nucleotides that contain the sugar ribose and one of the four bases cytosine, adenine, guanine and uracile Polynucleotide containing ribose sugar and uracile instead of thymine Primary agent for transferring information from the genome to the protein synthetic machinery Types of RNA Three types of RNA: messenger RNA (mRNA) a) b) c) transfer RNA (tRNA) ribosome RNA (rRNA) Remember: All produced in the nucleus Codon There are 20 different possible amino acids to make from different codons Amino acids: the building of protein 3 possible stop codon 1 start codon TAC on DNA AUG on RNA Gene Expression Production of proteins requires two steps: Transcription involves an enzyme (RNA polymerase) making an RNA copy of part of one DNA strand. There are four main classes of RNA: i. Messenger RNAs (mRNA), which specify the amino acid sequence of a protein by using codons of the genetic code. ii. Transfer RNAs (tRNA). iii. Ribosomal RNAs (rRNA). Translation converts the information in mRNA into the amino acid sequence of a protein using ribosomes, large complexes of rRNAs and proteins. Steps of gene expression Transcription – DNA is read to make a mRNA in the nucleus of cells Translation – Reading the mRNA to make a protein in the cytoplasm Poly-peptide A primary structure of a protein A sequence of amino acid bonded together by peptide bonds. aa2 aa1 aa3 aa4 aa5 aa199 aa200 Protein A polymer of amino acids which may consists of one or more polypeptide chains Protein may be water insoluble and serve a structural role or be water soluble with catalytic activity