Download Cellular Respiration

Ch.9
Cellular Respiration
A.P. Biology
What’s the
point?
The point
is to make
ATP!
ATP
2006-2007
Harvesting stored energy
• Glucose is the model
respiration
– catabolism of glucose to produce ATP
glucose + oxygen  energy + water + carbon
dioxide
C6H12O6 + 6O2
 ATP + 6H2O + 6CO2 + heat
COMBUSTION = making a lot of heat energy RESPIRATION = making ATP (& some heat)
by burning fuels in one step
by burning fuels in many small steps
ATP
enzymes
fuel
(carbohydrates)
O2
O2
CO2 + H2O + ATP (+ heat)
glucose
CO2 + H2O + heat
ATP
How do we harvest energy from fuels?
• Digest large molecules into smaller ones
– break bonds & move electrons from one molecule to
another
• as electrons move they “carry energy” with them
• that energy is stored in another bond,
released as heat or harvested to make ATP
loses e-
gains e-
+
oxidized
+
–
+
e-
oxidation
e-
reduced
e-
reduction
redox
How do we move electrons in biology?
• Moving electrons in living systems
– electrons cannot move alone in cells
• electrons move as part of H atom
• move H = move electrons
loses e-
gains e-
oxidized
+
+
oxidation
e
p
reduced
+
–
H
reduction
H
oxidation
C6H12O6 + 6O2
H e-
 6CO2 + 6H2O + ATP
reduction
Oxidation & reduction
• Oxidation
–
–
–
–
–
adding O
removing H
loss of electrons
releases energy
exergonic
• Reduction
–
–
–
–
–
removing O
adding H
gain of electrons
stores energy
endergonic
oxidation
C6H12O6 + 6O2
 6CO2 + 6H2O + ATP
reduction
like $$
in the bank
Moving
electrons
in
respiration
• Electron carriers move electrons by
shuttling H atoms around
– NAD+  NADH (reduced)
– FAD+2  FADH2 (reduced)
NAD+
nicotinamide
Vitamin B3
niacin
O–
O– P
phosphates
–O
O
O–
O– P
O
–O
H
N+
NADH
O
C
+
reducing power!
H H
NH2
H
O
C
reduction
oxidation
N+
O–
O– P
O
–
O
adenine
O– P
O
ribose sugar carries electrons as
a reduced molecule
–O
–O
NH
How efficient!
Build once,
use many ways
Cellular respiration: overview
• 1.Glycolysis: cytosol
(cytoplasm); degrades
glucose into pyruvate
• 2.Kreb’s Cycle:
mitochondrial matrix;
pyruvate into carbon
dioxide
• 3.Electron Transport
Chain: inner membrane
of mitochondrion;
electrons passed to
oxygen
What’s the
point?
The point
is to make
ATP!
ATP
2006-2007
Glycolysis
• Breaking down glucose
– “glyco – lysis” (splitting sugar)
glucose      pyruvate
2x 3C
6C
– it’s inefficient
• generate only 2 ATP for every 1 glucose
That’s not enough
ATP for me!
In the
cytosol?
Why does
that make
evolutionary
sense?
Evolutionary perspective
Enzymes
• Prokaryotes
of glycolysis are
“well-conserved”
– first cells had no organelles
• Anaerobic atmosphere
– life on Earth first evolved without free oxygen (O2) in
atmosphere
– energy had to be captured from organic molecules in absence
of O2
• Prokaryotes that evolved glycolysis are ancestors of all
modern life
– ALL cells still utilize glycolysis
You mean
we’re related?
Do I have to invite
them over for
the holidays?
Overview
glucose
C-C-C-C-C-C
enzyme
10 reactions
– convert
glucose (6C) to
2 pyruvate (3C)
– produces:
4 ATP & 2 NADH
– consumes:
2 ATP
– net yield:
2 ATP & 2 NADH
2
2
enzyme
fructose-1,6bP
P-C-C-C-C-C-C-P
enzyme
ADP
enzyme
enzyme
DHAP
P-C-C-C
DHAP = dihydroxyacetone phosphate
G3P = glyceraldehyde-3-phosphate
ATP
G3P
C-C-C-P
2H
2Pi enzyme
2
NAD+
2
enzyme
2Pi
4
ADP
enzyme
pyruvate
C-C-C
4
ATP
Is that all there is?
• Not a lot of energy…
– for 1 billon years+ this is how life on Earth
survived
• no O2 = slow growth, slow reproduction
• only harvest 3.5% of energy stored in glucose
– more carbons to strip off = more energy to harvest
O2
O2
O2
O2
O2
glucose     pyruvate
2x 3C
6C
Hard way
to make
a living!
But can’t stop there!
G3P
DHAP
NAD+
raw materials  products
Pi
+
NADH
NAD
NADH
Pi
1,3-BPG
NAD+
Pi
+
NADH
NAD
1,3-BPG
NADH
7
ADP
Glycolysis
6
Pi
ADP
ATP
ATP
3-Phosphoglycerate
(3PG)
glucose + 2ADP + 2Pi + 2 NAD+  2 pyruvate + 2ATP + 2NADH
3-Phosphoglycerate
(3PG)
8
• Going to run out of NAD+
– without regenerating NAD+,
energy production would stop!
– another molecule must accept H
from NADH
• so NAD+ is freed up for another round
2-Phosphoglycerate
(2PG)
H2O
9
Phosphoenolpyruvate
(PEP)
2-Phosphoglycerate
(2PG)
H2O
Phosphoenolpyruvate
(PEP)
10
ADP
ADP
ATP
ATP
Pyruvate
Pyruvate
Pyruvate is a branching point
Pyruvate
O2
O2
fermentation
anaerobic
respiration
mitochondria
Krebs cycle
aerobic respiration
How is NADH recycled to NAD+?
Another molecule
must accept H from
NADH
H 2O
O2
recycle
NADH
without oxygen
with oxygen
anaerobic respiration
aerobic respiration
“fermentation”
pyruvate
NAD+
NADH
acetyl-CoA
CO2
NADH
NAD+
lactate
which path you
use depends
on who you
are…
acetaldehyde
NADH
NAD+
lactic acid
fermentation
Krebs
cycle
ethanol
alcohol
fermentation
Fermentation (anaerobic)
• Plants, bacteria, yeast
pyruvate  ethanol + CO2
3C
NADH
 beer, wine, bread
2C
NAD+
1C
back to glycolysis
 Animals, some fungi
pyruvate  lactic acid
3C
NADH
3C
NAD+
back to glycolysis
 cheese, anaerobic exercise (no O2)
Alcohol Fermentation
pyruvate  ethanol + CO2
3C
NADH
 Dead end
process
2C
1C
NAD+back to glycolysis
 at ~12%
ethanol, kills
yeast
 can’t reverse the
reaction
recycle
NADH
bacteria
yeast
animals
some fungi
Lactic Acid Fermentation
recycle
pyruvate  lactic acid

3C
NADH
3C
NAD+back to glycolysis
 Reversible process
 once O2 is
available, lactate is
converted back to
pyruvate by the
liver
Count the
carbons!
O2
NADH
Pyruvate is a branching point
Pyruvate
O2
O2
fermentation
anaerobic
respiration
mitochondria
Krebs cycle
aerobic respiration
Glycolysis is only the start
• Glycolysis
glucose      pyruvate
6C
2x 3C
• Pyruvate has more energy to yield
– 3 more C to strip off (to oxidize)
– if O2 is available, pyruvate enters mitochondria
– enzymes of Krebs cycle complete the full oxidation of
sugar to CO2
pyruvate       CO2
3C
1C
Oxidation of pyruvate
• Pyruvate enters mitochondrial matrix
[
]
2x pyruvate    acetyl CoA + CO2
3C
2C
1C
–
–
–
–
NAD
3 step oxidation process
releases 2 CO2 (count the carbons!)
reduces 2 NAD  2 NADH (moves e-)
produces 2 acetyl CoA
• Acetyl CoA enters Krebs cycle
Where
does the
CO2 go?
Exhale!
1937 | 1953
Krebs cycle
• aka Citric Acid Cycle
– in mitochondrial matrix
– 8 step pathway
• each catalyzed by specific enzyme
• step-wise catabolism of 6C citrate molecule
Hans Krebs
1900-1981
• Evolved later than glycolysis
– does that make evolutionary sense?
• bacteria 3.5 billion years ago (glycolysis)
• free O2 2.7 billion years ago (photosynthesis)
• eukaryotes 1.5 billion years ago (aerobic respiration =
organelles  mitochondria)
Count the carbons!
pyruvate
3C
2C
6C
4C
This happens
twice for each
glucose
molecule
4C
acetyl CoA
citrate
oxidation
of sugars
CO2
x2
4C
4C
6C
5C
4C
CO2
Count the electron carriers!
pyruvate
3C
FADH2
6C
4C
NADH
This happens
twice for each
glucose
molecule
2C
4C
acetyl CoA
citrate
reduction
of electron
carriers
x2
4C
4C
ATP
CO2
NADH
6C
CO2
NADH
5C
4C
CO2
NADH
Energy accounting of Krebs cycle
4 NAD + 1 FAD
4 NADH + 1 FADH2
2x pyruvate          CO2
3C
3x 1C
1 ADP
1 ATP
ATP
Net gain = 2 ATP
= 8 NADH + 2 FADH2
ATP accounting so far…
• Glycolysis  2 ATP
• Kreb’s cycle  2 ATP
• Life takes a lot of energy to run, need to
extract more energy than 4 ATP!
There’s got to be a better way!
I need a lot
more ATP!
A working muscle recycles
over
10 million ATPs per second
Whassup?
So we fully
oxidized
glucose
C6H12O6

CO2
& ended up
with 4 ATP!
What’s the
point?
Electron Carriers = Hydrogen Carriers
H+
 Krebs cycle
produces large
quantities of
electron carriers
NADH
 FADH2
 go to Electron
Transport
Chain!

What’s so
important about
electron carriers?
H+
+
H+ H+ H
+
H+ H H+
ADP
+ Pi
ATP
H+
There is a better way!
• Electron Transport Chain
– series of proteins built into
inner mitochondrial membrane
• along cristae
• transport proteins & enzymes
– transport of electrons down ETC linked to pumping
of H+ to create H+ gradient
– yields ~34 ATP from 1 glucose!
– only in presence of O2 (aerobic respiration)
That
sounds more
like it!
O2
Remember the Electron Carriers?
Glycolysis
glucose
Krebs cycle
G3P
2 NADH
Time to
break open
the piggybank!
8 NADH
2 FADH2
Electron Transport Chain
Building proton gradient!
NADH  NAD+ + H
e
p
intermembrane
space
H+
H+
H  e- + H+
H+
C
e–
Q
e–
NADH H
FADH2
NAD+
NADH
dehydrogenase
inner
mitochondrial
membrane
e–
H
FAD
2H+ +1
O2
2
cytochrome
bc complex
H 2O
cytochrome c
oxidase complex
mitochondrial
matrix
Stripping H from Electron Carriers
• Electron carriers pass electrons & H+ to ETC
– H cleaved off NADH & FADH2
– electrons stripped from H atoms  H+ (protons)
• electrons passed from one electron carrier to next in mitochondrial
membrane (ETC)
• flowing electrons = energy to do work
– transport proteins in membrane pump H+ (protons) across
inner membrane to intermembrane space
TA-DA!!
Moving electrons
do the work!
H
H
+
H+
H+
H
+
H+
+
H+
H+
+
H+ H+ H
+
H+ H H+
C
e–
Q
e–
FADH2
FAD
e–
1
NADH
2H+ +2 O2
H2O
NAD+
cytochrome
NADH
cytochrome c
bc complex
dehydrogenase
oxidase complex
ADP
+ Pi
ATP
H+
But what “pulls” the
electrons down the ETC?
O2
electrons
flow downhill
to O2
H 2O
oxidative phosphorylation
Electrons flow downhill
• Electrons move in steps from
carrier to carrier downhill to oxygen
– each carrier more electronegative
– controlled oxidation
– controlled release of energy
make ATP
instead of
fire!
“proton-motive” force
We did it!
H+
• Set up a H+ gradient
• Allow the protons
to flow through
ATP synthase
• Synthesizes ATP
H+
H+
H+
H+
H+
H+
H+
ADP + Pi  ATP
ADP + Pi
Are we
there yet?
ATP
H+
Chemiosmosis
• The diffusion of ions across a membrane
– build up of proton gradient just so H+ could flow through
ATP synthase enzyme to build ATP
Chemiosmosis
links the
Electron
Transport Chain
to ATP synthesis
So that’s
the point!
Pyruvate from
cytoplasm
Inner
+
mitochondrial H
membrane
H+
Intermembrane
space
Electron
transport
C system
Q
NADH
Acetyl-CoA
2. Electrons
provide energy
1. Electrons are harvested
to pump protons
and carried to the
across the
membrane.
transport system.
NADH
Krebs
cycle
e-
e-
FADH2
e-
3. Oxygen joins
with protons to
form water.
H+
CO2
ATP
Mitochondrial
matrix
H2 O
ATP
ATP
4. Protons diffuse back in
down their concentration
gradient, driving the
synthesis of ATP.
1O
2 +2
2H+
H+
e-
O2
H+
ATP
synthase
Taking it beyond…
H+
H+
• What is the final electron acceptor in
Electron Transport Chain? e Q e
H+
C
–
–
O2
NADH
FADH2
NAD+
NADH
dehydrogenase
e–
FAD
1
2H+ +2 O2
cytochrome
bc complex
 So what happens if O2 unavailable?
 ETC backs up
nothing to pull electrons down
chain
 NADH & FADH2 can’t unload H

 ATP production ceases
 cells run out of energy
 and you die!
H2O
cytochrome c
oxidase complex
What’s the
point?
The point
is to make
ATP!
ATP
2006-2007
Review: Cellular Respiration
• Glycolysis:
2 ATP (substrate-level
phosphorylation)
• Kreb’s Cycle:
2 ATP (substrate-level
phosphorylation)
• Electron transport & oxidative
phosphorylation:
2 NADH (glycolysis) = 6 ATP
2 NADH (acetyl CoA) = 6ATP
6 NADH (Kreb’s) = 18 ATP
2 FADH2 (Kreb’s) = 4 ATP
• 38 TOTAL ATP/glucose