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Ch.9 Cellular Respiration A.P. Biology What’s the point? The point is to make ATP! ATP 2006-2007 Harvesting stored energy • Glucose is the model respiration – catabolism of glucose to produce ATP glucose + oxygen energy + water + carbon dioxide C6H12O6 + 6O2 ATP + 6H2O + 6CO2 + heat COMBUSTION = making a lot of heat energy RESPIRATION = making ATP (& some heat) by burning fuels in one step by burning fuels in many small steps ATP enzymes fuel (carbohydrates) O2 O2 CO2 + H2O + ATP (+ heat) glucose CO2 + H2O + heat ATP How do we harvest energy from fuels? • Digest large molecules into smaller ones – break bonds & move electrons from one molecule to another • as electrons move they “carry energy” with them • that energy is stored in another bond, released as heat or harvested to make ATP loses e- gains e- + oxidized + – + e- oxidation e- reduced e- reduction redox How do we move electrons in biology? • Moving electrons in living systems – electrons cannot move alone in cells • electrons move as part of H atom • move H = move electrons loses e- gains e- oxidized + + oxidation e p reduced + – H reduction H oxidation C6H12O6 + 6O2 H e- 6CO2 + 6H2O + ATP reduction Oxidation & reduction • Oxidation – – – – – adding O removing H loss of electrons releases energy exergonic • Reduction – – – – – removing O adding H gain of electrons stores energy endergonic oxidation C6H12O6 + 6O2 6CO2 + 6H2O + ATP reduction like $$ in the bank Moving electrons in respiration • Electron carriers move electrons by shuttling H atoms around – NAD+ NADH (reduced) – FAD+2 FADH2 (reduced) NAD+ nicotinamide Vitamin B3 niacin O– O– P phosphates –O O O– O– P O –O H N+ NADH O C + reducing power! H H NH2 H O C reduction oxidation N+ O– O– P O – O adenine O– P O ribose sugar carries electrons as a reduced molecule –O –O NH How efficient! Build once, use many ways Cellular respiration: overview • 1.Glycolysis: cytosol (cytoplasm); degrades glucose into pyruvate • 2.Kreb’s Cycle: mitochondrial matrix; pyruvate into carbon dioxide • 3.Electron Transport Chain: inner membrane of mitochondrion; electrons passed to oxygen What’s the point? The point is to make ATP! ATP 2006-2007 Glycolysis • Breaking down glucose – “glyco – lysis” (splitting sugar) glucose pyruvate 2x 3C 6C – it’s inefficient • generate only 2 ATP for every 1 glucose That’s not enough ATP for me! In the cytosol? Why does that make evolutionary sense? Evolutionary perspective Enzymes • Prokaryotes of glycolysis are “well-conserved” – first cells had no organelles • Anaerobic atmosphere – life on Earth first evolved without free oxygen (O2) in atmosphere – energy had to be captured from organic molecules in absence of O2 • Prokaryotes that evolved glycolysis are ancestors of all modern life – ALL cells still utilize glycolysis You mean we’re related? Do I have to invite them over for the holidays? Overview glucose C-C-C-C-C-C enzyme 10 reactions – convert glucose (6C) to 2 pyruvate (3C) – produces: 4 ATP & 2 NADH – consumes: 2 ATP – net yield: 2 ATP & 2 NADH 2 2 enzyme fructose-1,6bP P-C-C-C-C-C-C-P enzyme ADP enzyme enzyme DHAP P-C-C-C DHAP = dihydroxyacetone phosphate G3P = glyceraldehyde-3-phosphate ATP G3P C-C-C-P 2H 2Pi enzyme 2 NAD+ 2 enzyme 2Pi 4 ADP enzyme pyruvate C-C-C 4 ATP Is that all there is? • Not a lot of energy… – for 1 billon years+ this is how life on Earth survived • no O2 = slow growth, slow reproduction • only harvest 3.5% of energy stored in glucose – more carbons to strip off = more energy to harvest O2 O2 O2 O2 O2 glucose pyruvate 2x 3C 6C Hard way to make a living! But can’t stop there! G3P DHAP NAD+ raw materials products Pi + NADH NAD NADH Pi 1,3-BPG NAD+ Pi + NADH NAD 1,3-BPG NADH 7 ADP Glycolysis 6 Pi ADP ATP ATP 3-Phosphoglycerate (3PG) glucose + 2ADP + 2Pi + 2 NAD+ 2 pyruvate + 2ATP + 2NADH 3-Phosphoglycerate (3PG) 8 • Going to run out of NAD+ – without regenerating NAD+, energy production would stop! – another molecule must accept H from NADH • so NAD+ is freed up for another round 2-Phosphoglycerate (2PG) H2O 9 Phosphoenolpyruvate (PEP) 2-Phosphoglycerate (2PG) H2O Phosphoenolpyruvate (PEP) 10 ADP ADP ATP ATP Pyruvate Pyruvate Pyruvate is a branching point Pyruvate O2 O2 fermentation anaerobic respiration mitochondria Krebs cycle aerobic respiration How is NADH recycled to NAD+? Another molecule must accept H from NADH H 2O O2 recycle NADH without oxygen with oxygen anaerobic respiration aerobic respiration “fermentation” pyruvate NAD+ NADH acetyl-CoA CO2 NADH NAD+ lactate which path you use depends on who you are… acetaldehyde NADH NAD+ lactic acid fermentation Krebs cycle ethanol alcohol fermentation Fermentation (anaerobic) • Plants, bacteria, yeast pyruvate ethanol + CO2 3C NADH beer, wine, bread 2C NAD+ 1C back to glycolysis Animals, some fungi pyruvate lactic acid 3C NADH 3C NAD+ back to glycolysis cheese, anaerobic exercise (no O2) Alcohol Fermentation pyruvate ethanol + CO2 3C NADH Dead end process 2C 1C NAD+back to glycolysis at ~12% ethanol, kills yeast can’t reverse the reaction recycle NADH bacteria yeast animals some fungi Lactic Acid Fermentation recycle pyruvate lactic acid 3C NADH 3C NAD+back to glycolysis Reversible process once O2 is available, lactate is converted back to pyruvate by the liver Count the carbons! O2 NADH Pyruvate is a branching point Pyruvate O2 O2 fermentation anaerobic respiration mitochondria Krebs cycle aerobic respiration Glycolysis is only the start • Glycolysis glucose pyruvate 6C 2x 3C • Pyruvate has more energy to yield – 3 more C to strip off (to oxidize) – if O2 is available, pyruvate enters mitochondria – enzymes of Krebs cycle complete the full oxidation of sugar to CO2 pyruvate CO2 3C 1C Oxidation of pyruvate • Pyruvate enters mitochondrial matrix [ ] 2x pyruvate acetyl CoA + CO2 3C 2C 1C – – – – NAD 3 step oxidation process releases 2 CO2 (count the carbons!) reduces 2 NAD 2 NADH (moves e-) produces 2 acetyl CoA • Acetyl CoA enters Krebs cycle Where does the CO2 go? Exhale! 1937 | 1953 Krebs cycle • aka Citric Acid Cycle – in mitochondrial matrix – 8 step pathway • each catalyzed by specific enzyme • step-wise catabolism of 6C citrate molecule Hans Krebs 1900-1981 • Evolved later than glycolysis – does that make evolutionary sense? • bacteria 3.5 billion years ago (glycolysis) • free O2 2.7 billion years ago (photosynthesis) • eukaryotes 1.5 billion years ago (aerobic respiration = organelles mitochondria) Count the carbons! pyruvate 3C 2C 6C 4C This happens twice for each glucose molecule 4C acetyl CoA citrate oxidation of sugars CO2 x2 4C 4C 6C 5C 4C CO2 Count the electron carriers! pyruvate 3C FADH2 6C 4C NADH This happens twice for each glucose molecule 2C 4C acetyl CoA citrate reduction of electron carriers x2 4C 4C ATP CO2 NADH 6C CO2 NADH 5C 4C CO2 NADH Energy accounting of Krebs cycle 4 NAD + 1 FAD 4 NADH + 1 FADH2 2x pyruvate CO2 3C 3x 1C 1 ADP 1 ATP ATP Net gain = 2 ATP = 8 NADH + 2 FADH2 ATP accounting so far… • Glycolysis 2 ATP • Kreb’s cycle 2 ATP • Life takes a lot of energy to run, need to extract more energy than 4 ATP! There’s got to be a better way! I need a lot more ATP! A working muscle recycles over 10 million ATPs per second Whassup? So we fully oxidized glucose C6H12O6 CO2 & ended up with 4 ATP! What’s the point? Electron Carriers = Hydrogen Carriers H+ Krebs cycle produces large quantities of electron carriers NADH FADH2 go to Electron Transport Chain! What’s so important about electron carriers? H+ + H+ H+ H + H+ H H+ ADP + Pi ATP H+ There is a better way! • Electron Transport Chain – series of proteins built into inner mitochondrial membrane • along cristae • transport proteins & enzymes – transport of electrons down ETC linked to pumping of H+ to create H+ gradient – yields ~34 ATP from 1 glucose! – only in presence of O2 (aerobic respiration) That sounds more like it! O2 Remember the Electron Carriers? Glycolysis glucose Krebs cycle G3P 2 NADH Time to break open the piggybank! 8 NADH 2 FADH2 Electron Transport Chain Building proton gradient! NADH NAD+ + H e p intermembrane space H+ H+ H e- + H+ H+ C e– Q e– NADH H FADH2 NAD+ NADH dehydrogenase inner mitochondrial membrane e– H FAD 2H+ +1 O2 2 cytochrome bc complex H 2O cytochrome c oxidase complex mitochondrial matrix Stripping H from Electron Carriers • Electron carriers pass electrons & H+ to ETC – H cleaved off NADH & FADH2 – electrons stripped from H atoms H+ (protons) • electrons passed from one electron carrier to next in mitochondrial membrane (ETC) • flowing electrons = energy to do work – transport proteins in membrane pump H+ (protons) across inner membrane to intermembrane space TA-DA!! Moving electrons do the work! H H + H+ H+ H + H+ + H+ H+ + H+ H+ H + H+ H H+ C e– Q e– FADH2 FAD e– 1 NADH 2H+ +2 O2 H2O NAD+ cytochrome NADH cytochrome c bc complex dehydrogenase oxidase complex ADP + Pi ATP H+ But what “pulls” the electrons down the ETC? O2 electrons flow downhill to O2 H 2O oxidative phosphorylation Electrons flow downhill • Electrons move in steps from carrier to carrier downhill to oxygen – each carrier more electronegative – controlled oxidation – controlled release of energy make ATP instead of fire! “proton-motive” force We did it! H+ • Set up a H+ gradient • Allow the protons to flow through ATP synthase • Synthesizes ATP H+ H+ H+ H+ H+ H+ H+ ADP + Pi ATP ADP + Pi Are we there yet? ATP H+ Chemiosmosis • The diffusion of ions across a membrane – build up of proton gradient just so H+ could flow through ATP synthase enzyme to build ATP Chemiosmosis links the Electron Transport Chain to ATP synthesis So that’s the point! Pyruvate from cytoplasm Inner + mitochondrial H membrane H+ Intermembrane space Electron transport C system Q NADH Acetyl-CoA 2. Electrons provide energy 1. Electrons are harvested to pump protons and carried to the across the membrane. transport system. NADH Krebs cycle e- e- FADH2 e- 3. Oxygen joins with protons to form water. H+ CO2 ATP Mitochondrial matrix H2 O ATP ATP 4. Protons diffuse back in down their concentration gradient, driving the synthesis of ATP. 1O 2 +2 2H+ H+ e- O2 H+ ATP synthase Taking it beyond… H+ H+ • What is the final electron acceptor in Electron Transport Chain? e Q e H+ C – – O2 NADH FADH2 NAD+ NADH dehydrogenase e– FAD 1 2H+ +2 O2 cytochrome bc complex So what happens if O2 unavailable? ETC backs up nothing to pull electrons down chain NADH & FADH2 can’t unload H ATP production ceases cells run out of energy and you die! H2O cytochrome c oxidase complex What’s the point? The point is to make ATP! ATP 2006-2007 Review: Cellular Respiration • Glycolysis: 2 ATP (substrate-level phosphorylation) • Kreb’s Cycle: 2 ATP (substrate-level phosphorylation) • Electron transport & oxidative phosphorylation: 2 NADH (glycolysis) = 6 ATP 2 NADH (acetyl CoA) = 6ATP 6 NADH (Kreb’s) = 18 ATP 2 FADH2 (Kreb’s) = 4 ATP • 38 TOTAL ATP/glucose