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PowerPoint® Lecture Slide Presentation by Patty Bostwick-Taylor, Florence-Darlington Technical College Cells and Tissues 3 PART B Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Cell Physiology: Membrane Transport Membrane transport—movement of substances into and out of the cell Two methods of transport Passive transport No energy is required, uses kinetic energy ( bouncing particles) Active transport Cell must provide metabolic energy (ATP) Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Selective Permeability The plasma membrane allows some materials to pass while excluding others Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Diffusion Movement from high concentration to low concentration, Till particles equally distributed Figure 3.9 Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Types of diffusion Simple diffusion: gases Small enough particles to pass through membrane pores Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Types of diffusion (continued) Osmosis—simple diffusion of water Isotonic Solution: no movement of water, Cell at Equilibrium: homeostasis Hypertonic Solution: solution outside of cell high in solute, low in water. Water flow out of cell, Cell Shrinks: Plasmolysis, ex dehydration Hypotonic Solution: solution outside of cell low in solute( pure water) Water flows in to reach balance, Too much- Cell Burst: Cytolysis ex: cut in bath Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Facilitated diffusion Substances require a protein carrier for passive transport Transports large substances Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Figure 3.10b–c Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Passive Transport Processes Filtration Water and solutes are forced through a membrane by fluid, or hydrostatic pressure A pressure gradient must exist Solute-containing fluid is pushed from a high-pressure area to a lower pressure area Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes Substances may be too large Substances may not be able to dissolve in the fat core of the membrane Substances may have to move against a concentration gradient ATP is used for transport Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes Two common forms of active transport Solute pumping Vesicular transport: Exocytosis Endocytosis Phagocytosis Pinocytosis Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes Active transport (solute pumping) Amino acids, some sugars, and ions are transported by protein carriers called solute pumps ATP energizes protein carriers In most cases, substances are moved against concentration gradients Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes Vesicular transport Exocytosis Moves materials out of the cell Material is carried in a membranous vesicle Material is emptied to the outside Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes: Exocytosis Figure 3.12b Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Active Transport Processes Vesicular transport (continued) Endocytosis Extracellular substances are engulfed by being enclosed in a membranous vescicle Types of endocytosis Phagocytosis—“cell eating” Pinocytosis—“cell drinking” Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Cell Life Cycle Cells have two major periods Interphase Cell grows Cell carries on metabolic processes Cell division Cell replicates itself Function is to produce more cells for growth and repair processes Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings DNA Replication Genetic material is duplicated and readies a cell for division into two cells Occurs toward the end of interphase DNA uncoils and each side serves as a template Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Events of Cell Division Mitosis—division of the nucleus Results in the formation of two daughter nuclei Cytokinesis—division of the cytoplasm Begins when mitosis is near completion Results in the formation of two daughter cells Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Prophase First part of cell division Centrioles migrate to the poles to direct assembly of mitotic spindle fibers DNA appears as double-stranded chromosomes Nuclear envelope breaks down and disappears Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Metaphase Chromosomes are aligned in the center of the cell on the metaphase plate Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Anaphase Chromosomes are pulled apart and toward the opposite ends of the cell Cell begins to elongate Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Telophase Chromosomes uncoil to become chromatin Nuclear envelope reforms around chromatin Spindles break down and disappear Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Cytokinesis Begins during late anaphase and completes during telophase A cleavage furrow forms to pinch the cells into two parts Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Centrioles Plasma membrane Centrioles Chromatin Forming mitotic spindle Nuclear envelope Nucleolus Early prophase Interphase Spindle Chromosome, consisting of two sister chromatids Centromere Spindle microtubules Fragments of nuclear envelope Centromere Spindle pole Late prophase Nucleolus forming Metaphase plate Cleavage furrow Sister chromatids Metaphase Daughter chromosomes Anaphase Nuclear envelope forming Telophase and cytokinesis Figure 3.15 Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings Stages of Mitosis Spindle Metaphase plate Sister chromatids Metaphase Figure 3.15, step 4 Copyright © 2009 Pearson Education, Inc., publishing as Benjamin Cummings