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1 What is this? 2 Microscopes Different types of microscopes – Light microscope-magnifies images by focusing light on them • Most commonly used • Compound- have two lenses • Stains may be added to make objects more visible – Electron microscopes- magnifies images a 1000X’s more than light microscopes by focusing a beam of electrons on them • SEM • TEM 3 4 A Foot of a Housefly Using SEM 5 Modern Cell Theory Cell Theory: – Cells are the smallest working units of life – All living things are made of cells – All cells come from preexisting cells Cheek cells 6 Cell Factory The cell is like a factory – People and machines perform functions in a factory – There are parts of a cell that also perform different functions 7 Cell Factory All factories produce a product. CELL PRODUCT=PROTEINS – Cell’s DNA codes for proteins! – All cells in an organism have the same DNA, but different cell types produce different proteins. – What is needed to build a protein? 8 4 Parts of All Cells 1. Cytoplasm- watery substance inside all cells 2. Cell membrane (plasma membrane)- “door” to the factory that’s made of lipids and proteins – It is a phospholipid bilayer – Selectively-permeableonly certain things pass through – Aids in homeostasis for stable internal conditions 9 Phospholipid Structure 10 4 Parts of All Cells 3. Ribosomes -“assembly line workers” – They build proteins – Found all over cell – Proteins are either used by cell or shipped out to other cells 4. Genetic material – DNA – RNA 11 Two Main Types of Cells Prokaryotic – Bacteria – Small – No organelles except ribosomes – No nucleus – Simple Eukaryotic – Anything other than bacteria, like plants and animals, have these cells – Large cells – Organelles – True nucleus – Complex 12 Eukaryotic Organelles Nucleus-“central office” of the cell that contains DNA where most cell activity is started – DNA carries instructions for how to build proteins – Nucleus is surrounded by a porous membrane. Why? – The nucleolus, is found in the nucleus and makes the ribosomes. 13 Eukaryotic Organelles Mitochondria- “powerhouse” of the cell – The process of cellular respiration releases chemical energy from carbs to make ATP when oxygen is present – C6H12O6 (glucose) + O2 → CO2 + H2O + energy(ATP) – Some cells have more than others—why? – Contain DNA also Oxygen and glucose in Carbon dioxide, water, and 14 ATP produced Eukaryotic Organelles Endoplasmic reticulum -“highway” system throughout inside of cell – Materials can travel on it throughout the cell – Rough ER (has ribosomes) vs. Smooth ER 15 Eukaryotic Organelles Golgi apparatus-“packaging center” of the cell – Puts finishing touches on proteins – Prepares them for shipment out of the cell 16 Eukaryotic Organelles Lysosome –“recycling center” – Repairs damaged cell parts. – Sometimes parts are beyond repair. 17 Eukaryotic Organelles Vacuoles“storerooms” in plant and animal cells. – What would they store? – If they shrivel, so does the cell 18 2 Types of Eukaryotic Cells There are two main types of eukaryotic cells – Plant-like – Animal-like 19 Plant-like Eukaryotic Cells Have: – Large vacuoles…Why? – A cell membrane that is surrounded by a cell wall • Made of cellulose, a carb • Gives plant cells support and structure which protect s cell from bursting when vacuoles swell with water – Chloroplasts so can make own carbs through photosynthesis • Chlorophyll-green pigment in chloroplasts that gathers sunlight • Have DNA like mitochondria 20 Photosynthesis Source of energy for ALL life on Earth!!! Reactants Products 1. Water 1. Oxygen gas 2. Carbon Dioxide 2. Glucose (a carb) 3. Sunlight 21 Photosynthesis 22 Animal-like Eukaryotic Cells Have: – Only a cell membrane – No chloroplasts for photosynthesis so must consume food for energy – Small vacuoles 23 Endosymbiotic Theory Endosymbiotic Theory -mitochondria and chloroplasts were once prokaryotic cells that were taken in by other prokaryotes. Creation of first eukaryotic “organelles” Prokaryotes evolved into eukaryotes Endosymbiotic Theory 24 25 Cell Transport Cells need: WATER water, oxygen, and nutrients to get in through the cell membrane so they WASTE may use them wastes, proteins, hormones, and other particles to leave Cell transport – the OXYGEN movement of particles through the cell membrane into or out of the cell NUTRIENTS CARBON DIOXIDE 26 Cell Membranes ALL CELLS HAVE A CELL MEMBRANE Cell membrane- two layered “door” to the factory that’s made of lipids and proteins Selectively-permeable-only certain things pass through Aids in homeostasis— maintaining stable conditions within the cell 27 Cell Membranes 28 Phospholipid Structure Each phospholipid of the cell membrane has two ends: = “water loving” head Hydrophobic = “water fearing” tail Hydrophilic 29 30 Cell Membrane Proteins Receptor proteins- allow cells to talk to one another Cells send “messages” and others receive them with receptors. Hormones-cellular messages Ex.) Testosterone 31 Cell Membrane Proteins Marker proteins -gives your cells an “identity” Distinguishes your cells from other cells Blood and tissue types Immune system A marker A and B markers B marker No marker 32 Cell Membrane Proteins Channel proteins allow molecules to pass through the membrane Create a channel through the lipids of the membrane Selective about what passes through 33 Natural Movement of Particles Things move naturally from high to low concentrations. Spray of Very low or no concentration perfume Fart Cooking popcorn Nutrients in bloodstream Start out highly concentrated, but molecules move outward to areas of low concentration High concentration 34 Which container has the highest concentration of particles? What would happen to the particles if we connected containers A and C? 35 Natural Movement of Particles Movement of particle across cells membranes work the same way The cell creates an environment inside that may be different from the outside If the concentrations are different between the inside of the cell and the outside, particles and molecules will move until they are equal inside and out 36 are molecules that can pass through the cell membrane is a cell membrane A) B) Draw arrows representing which way the molecules will naturally move 37 Particles Different particles are transported differently across the cell membrane The cell membrane is selective Smaller molecules can pass through Larger molecules and charged molecules cannot. 38 Cell Transport Two types of transport of particles across the cell membrane: Passive—no energy (ATP) Active—takes energy (ATP) 39 Passive vs. Active Transport Passive transport – type of movement of particles across the cell membrane from high to low concentrations with no energy (ATP) needed “Paddling with the current” Types of passive transport Diffusion Osmosis Active transport is just the opposite…low to high that requires energy 40 Passive Transport -Diffusion Diffusion – natural movement of particles from high to low concentration Passive-no ATP needed Occurs until equilibrium is reached This is how some things are transported through the cell membrane High oxygen concentration in blood 41 from breathing diffuses into blood cells Passive Transport -Osmosis Why do I care? Osmosis-diffusion of water across the cell membrane from high to low concentrations Passive-no ATP needed Water passes easily through the semipermeable cell membrane. 42 Is There Such a Thing as Too Much Water? Water can go through the cell membrane very easily High concentrations of water in your blood will move into your cells Why should I care? 43 44