Download Full characterization of the first human umbilical cord blood Multi

Full characterization of the first human umbilical cord blood Multi Lineage
Progenitor Cell line.
Nicolas Forraz, Dan Collins, Marc-Olivier Baradez, Colin P.McGuckin.
Stem Cell Therapy Programme, Kingston University, London, KT1 2EE, UK and Bio-E
Incorporated, MN, USA.
Objectives: Tissue engineering and regenerative medicine protocols have been held back
through access to enough primary tissue for research and development. This study
characterized the first commercially available clonal multi-potent Multi Lineage
Progenitor Cell line (MLPC, Bio-E Inc, MN, USA) isolated from umbilical cord blood
(UCB). We compared the phenotype of the MLPC line to cell groups at various stages of
differentiation: UCB mononucleated cells, PrepaCyte®-purified cells, CD133+
progenitor cells, lineage-restricted stem cells and a bone marrow mesenchymal stem cell
(BMMSCs) line by high definition microarray. Methods: Following extraction
(Qiagen,USA) , RNA samples were hybridized to the PIQOR™ 942 gene Human Stem
Cell Microarray platform (Miltenyi Biotec, CA, USA). Bioinformatics interpretation
included full pathway analysis and signal network analysis comparing MLPC’s to each of
the five cell groups. Results: Bioinformatics gene expression analysis identified 631
genes to be significantly differentially expressed between MLPC’s (>1.4 fold up- or
down-regulated) and the other five cell groups. Pathway and signal network analysis
unravelled numerous biological pathways characterizing MLPCs unique features. MLPCs
demonstrated a high degree of stemness and quiescence: downregulation of 65 genes
associated with active protein synthesis (e.g. ribosomal sub-units), 18 genes linked with
phosphate metabolism (kinases and phosphatases), 123 genes regulating proliferation and
cell cycling (cyclins, cyclin-dependent kinases, check point proteins). MLPCs exhibited
differentiation multipotential: downregulation of 12 different cluster of differentiation
surface marker genes (e.g. epithelium, endothelium) but upregulation of 80 genes
involved in nucleic acid binding and/or transcription factor activity regulating
differentiation of tissues from all three germ layers. Conclusions: Microarray analysis
has revealed the Bio-E MLPC cell line to be of an extremely immature phenotype
significantly different to that of the bone marrow mesenchymal cell population and
MLPC cells are an ideal tool for the advancement of protocols in tissue engineering and
regenerative medicine.