Download PROFILING TECHNIQUE: MICROARRAY ANALYSIS

Survey
yes no Was this document useful for you?
   Thank you for your participation!

* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project

Document related concepts

Gene prediction wikipedia, lookup

Transcript
PROFILING TECHNIQUE: MICROARRAY ANALYSIS
http://learn.genetics.utah.edu/content/labs/microarray/
DNA microarray analysis is a technique that scientists use to determine whether genes are on or off.
The human genome contains approximately 20,000 genes. At any given moment, each of our cells has some
combination of these genes turned on, and others are turned off. How do scientists figure out which are on and which are
off?
Scientists can answer this question for any cell sample
or tissue by gene expression profiling, using a
technique called microarray (pronounced MY-crow-ahray) analysis
Microarray analysis involves breaking open a cell,
isolating its genetic contents, identifying all the genes
that are turned on in that particular cell and generating
a list of those genes.
What is a DNA Microarray?
A DNA micorarray allows scientists to perform an
experiment on thousands of genes at the same
time.
Each spot on a microarray contains multiple
identical strands of DNA.
The DNA sequence on each spot is unique.
Each spot represents one gene.
Thousands of spots are arrayed in orderly rows
and columns on a solid surface (usually glass).
The precise location and sequence of each spot
is recorded in a computer database.
Microarrays can be the size of a microscope
slide, or even smaller.