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Name ___________________________________________ Date ______Period # _____ From DNA to Protein Worksheet Write the complementary DNA strand to this leading strand of DNA below it. 5’ T A C C T G C C A G T T A T C G A G G C T A T C C G A T C C C G T T A T 3’ 3’ ___________________________________________________________________ 5’ Write the complementary mRNA strand to this strand of DNA below it. 5’ T A C C T G C C A G T T A C C G A G G C T A T G C G A T C C C G T A C T 3’ _______________________________________________________________________ Match codons of the mRNA strand you’ve created with their corresponding amino acids written on the line below them. Use the 1st 3 letters of the amino acid as abbreviations. The genetic code is found on page 207 of your text and on page 3 of accompanying WS. ________________________________________________________________________ ________________________________________________________________________ Using the following polypeptide sequence, predict 2 possible DNA sequences that could’ve coded for it. Met – Pro – Leu – Lys – His – Thr – Phe – Val – Ser – Gly –Ala – Tyr – STOP mRNA _________________________________________________________________ DNA __________________________________________________________________ mRNA (2)_______________________________________________________________ DNA (2)________________________________________________________________ Ala = alanine Gly – glycine Met = methionine His – histidine Leu – leucine Phe – phenylalanine Tyr = tyrosine Val – valine Pro – proline Glu – glutamic acid Lys = lysine Gln- glutamine Ser- serine Thr = threonine Asp- aspartic acid Asn - Asparagine Holts’ Chapter Test A: This is copyrighted & may not be reproduced. DNA, RNA, and Protein Synthesis In the space provided, write the letter of the description that best matches the term or phrase. ______ 1. nucleotide ______ 2. deoxyribose ______ 3. adenine ______ 4. guanine ______ 5. cytosine ______ 6. thymine ______ 7. purines ______ 8. pyrimidines ______ 9. DNA polymerases ______ 10. replication forks ______ 11. codon ______ 12. genome a. a nitrogenous base that forms hydrogen bonds with cytosine b. a nitrogenous base that forms hydrogen bonds with guanine c. a nitrogenous base that forms hydrogen bonds with thymine d. enzymes that have a proofreading role in DNA replication e. a class of organic molecules, each having a double ring of carbon and nitrogen atoms f. portions of DNA where the double helix separates during DNA replication g. a five-carbon sugar h. consists of a phosphate group, a sugar molecule, and a nitrogen base i. a nitrogenous base that forms hydrogen bonds with adenine j. a class of organic molecules, each having a single ring of carbon and nitrogen atoms k. complete gene content of an organism l. 3-nucleotide sequence of mRNA In the space provided, write the letter of the term or phrase that best completes each statement or best answers each question. ______ 13. What did Griffith observe in his transformation experiments? a. Virulent bacteria changed into harmless bacteria. b. Heat-killed bacteria changed into S bacteria. c. Harmless bacteria changed into S bacteria. d. Virulent S bacteria changed into harmless bacteria. ______ 14. In 1944, Avery conducted a series of experiments that showed that the material responsible for transformation is a.RNA. b. DNA. c. protein. d. bacteriophage. ______ 15 The nucleotides in DNA are bonded to each other by a. covalent bonds. c. ionic bonds. b. hydrogen bonds. d. peptide bonds. DNA, RNA, and Protein Synthesis, Chapter Test A continued Refer to the mRNA sequence CUC-AAG-UGC-UUC and the table below, which lists mRNA codons, to answer questions 14–16. _____ 16. Which of the following would represent the sequence of DNA from which the mRNA sequence was made? a. CUC-AAG-UGC-UUC c. GAG-TTC-ACG-AAG b. GAG-UUC-ACG-AAG d. AGA-CCT-GTA-GGA _____ 17. The anticodons for the codons in the mRNA sequence above are a. GAG-UUC-ACG-AAG. c. CUC-GAA-CGU-CUU. b. GAG-TTC-ACG-AAG. d. CUU-CGU-GAA-CUC. _____ 18. Which of the following represents the portion of the protein molecule coded for by the mRNA sequence above? a. serine-tyrosine-arginine-glycine b. valine-aspartic acid-proline-histidine c. leucine-lysine-cysteine-phenylalanine d. glutamic acid-phenylalanine-threonine-lysine _____ 19. The two chains of the DNA molecule are held together by a. covalent bonds. c. ionic bonds. b. hydrogen bonds. d. peptide bonds. _____ 20. At the end of the replication process, each of the two new DNA molecules is composed of which of the following? a. two new DNA strands b. one new and one original DNA strand c. one new and one mutated DNA strand d. two original DNA strands DNA, RNA, and Protein Synthesis, Chapter Test A continued Read each question, and write your answer in the space provided. 21. What is the difference between transcription and translation? _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ 22. Explain how RNA differs from DNA. _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ 23. Describe the role of DNA helicases during replication. _______________________________________________________________ _______________________________________________________________ _______________________________________________________________ Refer to the figure below to answer questions 24 and 25. C 24. What does the figure above represent? _______________________________________________________________ 25. Identify the structures labeled A–C.