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Station 1: Cork cells In 1665, Robert Hooke used an early compound microscope to look at a thin slice of cork. Under the microscope, the cork seemed to made of thousands tiny, empty chambers. Hooke called these chambers “cells” because they reminded him of a monastery’s tiny rooms, which were called cells. Cork is made from wood and was at one time living, although it is no longer considered alive when it is in cork form. As you know, all living things are made of cells and this wood is no exception. The cells are square- shaped and have a rigid outer wall (the cell wall, found in plant cells). It is the remains of the cell walls that you are seeing, which make the small box shapes. 400X Station 2: Cheek cells The internal parts of the cells, the organelles, are so transparent (clear) that they are often difficult to see. Biologists have developed a number of stains that help them see the cells and their organelles by adding color to their transparent parts. These cells have been stained with methylene blue stain. This stain sticks particularly well to DNA so you should be able to see the nucleus of the cells. The other organelles and parts are too small and not stained properly to see. These cells are thin with interlocking edges so that they can form a flexible barrier inside your mouth. This is just one way that cheek cells have structure related to their functions of protection and barrier. 400X Station 3: Bacteria shapes (spiral) Most bacteria come in three shapes: spherical, rod, and spiral. Can you tell which type these bacteria are? These are spiral-shaped bacteria and are generally the largest of the three common shapes. Streptococcus spp. (species) are chain-forming, sphere-shaped bacteria responsible for ailments such as strep throat in humans. Remember that bacteria are prokaryotes and are generally much smaller than plant and animal cells (eukaryotic cells). Can you see a difference in their size? Below is a picture showing the three shapes of bacteria. Sphere shaped Spiral Shaped 1000x Rod shaped Bacteria capsules This slide shows several bacteria that have capsules. Remember that bacteria are generally simple structures. The bacterial cell lacks a membrane-bound nucleus. Because of this, bacteria are described as prokaryotes. Bacteria are normally unicellular organisms that grow in colonies with other bacteria. Some bacteria are enclosed within a capsule – a hard protective coating surrounding the cell wall and membrane. This protects the bacteria. Bacteria may also have cilia and flagella to help them move in their environment. Do you remember what these look like? . 400x flagella Station 4: Plant leaf This is a leaf from the aquatic plant growing in our classroom. This cell has lots of chloroplast because it is responsible for making the food for the entire plant. Like any complex cell, it has the basic organelles. You will not see the nucleus and the DNA in this lab, but the cell wall and chloroplasts will be very obvious. The chloroplasts are the site for photosynthesis in the cell; they contain chlorophyll (a green pigment), which traps sunlight and thus enables the cell to combine carbon dioxide and water to make glucose. This is the process where plants take inorganic carbon dioxide and use the sun’s energy to make carbohydrates. 400x Station 5: Muscle cell – smooth muscle This is a muscle cell. Remember that muscle is a tissue made up of several cells all working together. Muscle cells can come in three types: heart muscle, smooth muscle, and skeletal muscle. This is an example of smooth muscle. Smooth muscle is made of single, string-shaped cells. Each smooth muscle cell contains thick (myosin) and thin (actin) filaments that slide against each other to produce a contraction in the cell. The nucleus in these cells are pushed off to the side and can be seen as the darker spots between the pink colored cells. These muscles help move blood in your body and help food move through your digestive system. The cells are long and thin shaped so that they can slide past each other to provide movement. This structure or shape is responsible for how the cells function and the job that they do. We cannot see the other organelles like mitochondria but we know that muscle cells need a lot of energy and probably have lots of mitochondria. Mitochondria are the organelles that provide the cell with energy. Label the cell membrane and nucleus in this tissue. Remember that you are seeing a lot of cells together but each cell has its own nucleus and membrane. 400x Station 6: Euglena Euglena are unicellular organisms that have a nucleus. They are in the kingdom Protista because of this. All euglena have chloroplasts and can make their own food by photosynthesis. On these slides we cannot see the chloroplasts because the cells have been died pink. Euglena move by a flagellum (plural ‚ flagella), which is a long whip-like structure that acts like a little motor. The flagellum is located on the anterior (front) end, and twirls in such a way as to pull the cell through the water. If you look closely at some of the Euglena cells you may be able to see the flagellum Euglena also have an eyespot at the anterior end that detects light, it can be seen near the reservoir. This helps the euglena find bright areas to gather sunlight to make their food. In the center of the cell is the nucleus, which contains the cell's DNA and controls the cell's activities. The dye used to prepare this slide colors the DNA a darker pink color than the rest of the cell. Make sure to look for this dark spon inside the cell. On your drawing label the cell membrane and nucleus. 400x