* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Pathophysiology of Heme Synthesis
Biochemistry wikipedia , lookup
Gene expression wikipedia , lookup
Clinical neurochemistry wikipedia , lookup
Lipid signaling wikipedia , lookup
Siderophore wikipedia , lookup
Peptide synthesis wikipedia , lookup
Proteolysis wikipedia , lookup
Biosynthesis wikipedia , lookup
Human iron metabolism wikipedia , lookup
Epitranscriptome wikipedia , lookup
Gaseous signaling molecules wikipedia , lookup
Messenger RNA wikipedia , lookup
Oligonucleotide synthesis wikipedia , lookup
Oxidative phosphorylation wikipedia , lookup
Amino acid synthesis wikipedia , lookup
De novo protein synthesis theory of memory formation wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Evolution of metal ions in biological systems wikipedia , lookup
Pathophysiology of Heme Synthesis Beth A. Bouchard BIOC 212: Biochemistry of Human Disease Spring 2006 HEME-CONTAINING PROTEINS Hemoglobin Myoglobin Cytochromes Catalase Some peroxidases STRUCTURE OF HEME Gut • Low pH of stomach solubilizes Fe-containing ionic compounds. • Fe transporters facilitate absorption into blood stream Blood • Fe3+ ions are bound and chelated by Transferrin (Tf). • Transferrin transports Fe to tissues •Maintains solubility •Keeps Fe ions unreactive Cells An Overview of Iron Metabolism • Transferrin endocytosis is receptor-mediated (TfR) • Endocytosis results in Fe3+ release • Fe is distributed to topologically distinct regions of the cell via Fe transporter and/or channels (?) • Usage: Protein components (Heme) • Storage: Ferritin (Fe2+) • Toxicity Roles of Iron in the Cell Fe(III)2-Tf Tf Transferrin Receptors (TfR) Proteins: Catalysis Electron, oxygen transport [Fe] Structural stabilization Sensor of Fe, ROS Formation of protein-bound radicals [Fe] Storage and Sequestration: Ferritin [Fe] Toxicity: Oxidative stress Iron Control of Translation • IREs are found in the 5’-UTR or the 3’UTR of mRNAs • Regulate mRNA translation via IRBP • Decreased cellular iron levels: – IRBP is free of iron and can therefore, interact with the IREs in the 3'-UTR of the transferrin receptor (TfR) mRNA, which prevents its degradation. – IRBP binds to the IRE in the 5’-UTR of the ferritin mRNA preventing its translation. • Increased cellular iron levels: – IRBP binds iron and cannot interact with the IREs in the TfR mRNA resulting in an increase in its degradation. – IRBP cannot bind to the IRE in the ferritin mRNA allowing for its translation. IRE STRUCTURE OF HEME • Ferrous iron (Fe2+) • Protoporphyrin IX: contains 4 pyrrole rings linked together by methenyl bridges HEME SYNTHESIS 8 8 Succinyl CoA Glycine** Heme ** Amino acid (building blocks of protein) synthesized in your body Heme synthesis HEME SYNTHESIS HEME SYNTHESIS: Red blood cells •85% of total heme synthesis occurs in red blood cells (RBC) •Ceases when RBC’s mature •Erythroid-specific ALA synthase is regulated by an IRE in the mRNA – binding of IRBP inhibits mRNA translation Heme stimulates hemoglobin synthesis in reticulocytes HCI = heme controlled inhibitor Reduced initiation of translation In RBCS, heme synthesis is also regulated at the level of the enzymes ferrochelatase* and porphobilinogen deaminase** * ** HEME SYNTHESIS: Liver •The liver is the main non-RBC source of heme synthesis •Heme produced in the liver is used mainly for the synthesis of the cytochrome P450 class of enzymes that are involved in detoxification Regulated at level of ALA synthase: Formation of 5ALA is the rate-limiting step in heme synthesis in the liver Formation of 5-aminolevulinate (5-ALA) 5-ALA 5-ALA is formed in the mitochondria and transported to the cytoplasm Regulation of ALA Synthase Level of enzyme synthesis Enzyme synthesis, as well as its transport to the mitochondria, is inhibited by elevated levels of heme and hemin, the Fe3+ oxidation product of heme Enzyme synthesis is upregulated by a large number of drugs including barbiturates, steroids with a 4,5 double bond (e.g. testosterone) and some oral contraceptives: These drugs are metabolized by the microsomal cytochrome P450 mono-oxygenase system, a hemecontaining protein. Level of enzyme activity Heme and hemin inhibit ALA synthase activity Requires pyridoxal phosphate (Vitamin B6) as a coenzyme Disorders of Heme Synthesis • Acquired: Lead poisoning • Congenital: Porphyrias • Deficiency of heme has far-reaching effects (hemoglobin, cytochromes, etc.) LEAD TOXICITY Symptoms Irritibility Lethargy Sleeplessness Headaches Pathophysoiology Poor appetite Abdominal pain (with or without vomiting) Constipation Binds to any compound with a sulfhydryl group Inhibits multiple enzyme reactions including those involved in heme biosynthesis (PBG synthase & ferrochelatase) One symptom of lead toxicity is increases in 5-ALA without concomitant increases in PBG HEME SYNTHESIS (CONT.) Vitamin B6 lead PORPHYRIAS A group of rare disorders caused by deficiencies of enzymes of the heme biosynthetic pathway The majority of the porphyrias are inherited in a autosomal dominant fashion - thus, affected individuals have 50% normal levels of the enzymes, and can still synthesize some heme Affected individuals have an accumulation of heme precursors (porphyrins), which are toxic at high concentrations Attacks of the disease are triggered by certain drugs, chemicals, and foods, and also by exposure to sun Treatment involves administration of hemin, which provides negative feedback for the heme biosynthetic pathway, and therefore, prevents accumulation of heme precursors Scriver et al., The Metabolic & Molecular Basis of Inherited Disease, 8th edition, 2001. ACUTE INTERMITTENT PORPHYRIA Hepatic, autosomal dominant Caused by a deficiency in porphobilinogen deaminase, which is involved in the conversion of porphobilinogen (PBG) to uroporphyrinogen III PBG, uroprophryin, and 5-ALA accumulate in the plasma and the urine Patients have neuropyschiatric symptoms and abdominal pain (neurovisceral) PORPHYRIA CUTANEA TARDA Most common porphyria Hepatic, autosomal dominant Disease is caused by a deficiency in uroporphyrinogen decarboxylase, which is involved in the conversion of uroporphyrinogen III to coproporphyrinogen III Uroporphyrinogen accumulates in urine Patients are photosensitive (cutaneous photosensitivity) Accumulation of porphyrinogens results in their conversion to porphyrins by light Porphyrins react with molecular oxygen to form oxygen radicals Oxygen radicals can cause severe damage to the skin