Download Protein Expression and Purification Service Quotation Request Form

Custom Antibody Information Form
Please provide the required information.
If you have any questions, please email us: [email protected] or call us: +333 90 20 54 70
Customer Information
Name:
Company:
Address:
Phone:
Fax:
Email:
(please provide at least country and postal code for shipping costs evaluation)
Antigen Information
Target Name:
NCBI Number:
Target Full Length Sequence:
Protein Description (e.g., whether it is a trans-membrane protein, whether it has a signal
peptide, etc):
Protein Localization:
Cell-membrane
Cytoplasm
Nucleus
Other:
If your protein is a trans membrane protein, do you know any extracellular domain?
amino acids; Location :
GI Number of proteins you would like the antibody not to recognize (if you don’t specify,
we will design the antigen so that the antibody will not recognize the most highly related
protein in NCBI):
Would you like to specifically detect a modification on the target protein?
Yes
No
Do you provide a plasmid containing the full-length cDNA?
Yes (please fill in Supporting Table 1)
No
Is your plasmid optimized for the production of the protein in a specific system?
Yes
No
If yes, specify the system:
Do you provide the peptide antigen?
If yes, please fill in Supporting Table 2
Yes
No
Do you provide the protein antigen?
Yes
No
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If yes, please fill in Supporting Table 3
Which species would you like to use?
Mouse
Rat
Either one
What application(s) you would use the antibody for?
ELISA
WB
FC or FACS
IF
IP
IHC
ELISA Sandwich
Other:
If several applications are needed, please mention the preferred one below (if any):
What kind of sample will the antibody be used on?
Additional relevant information regarding the final application, the target protein, the
hybridoma development process…
Final amount of purified antibody required:
Strategy for the final hybridoma/clones cell lines storage :
Cell lines storage in both customer facilities and ProteoGenix facilities (recommended)
Cell lines storage in ProteoGenix facilities only
Cell lines storage in customer facilities only
Supporting Table 1: Plasmid with cDNA provided by customers
cDNA sequence (ORF) :
Plasmid sequence:
Cloning strategy:
If the ORF sequence in the plasmid is different from the NCBI sequence, please specify:
Vector description:
Resistance (or other selection marker):
cDNA NCBI number:
Tag:
Tag position:
N-terminal
C-terminal
QC Standard:
Provided plasmids should have the correct sequence with sequencing report attached.
Plasmid quantity ≥ 5µg and concentration ≥ 10 ng/μl.
(Note: incorrect plasmids may results in additional fees and project delays or cancellation).
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Supporting Table 2: Peptide provided by customers
Unmodified peptide
Peptide sequence:
Peptide purity:
Modified peptide
Modified peptide sequence:
Modified peptide purity:
Protein Localization:
Cell-membrane
Cytoplasm
Nucleus
Other:
Unmodified control peptide sequence:
Unmodified control peptide purity:
QC Standard:
For monoclonal projects: ≥ 5 mg peptide, QC report (MS+HPLC), theoretical MW, purity ≥ 80%
For modification-specific projects: ≥ 10 mg modified peptide, ≥ 10 mg control peptide, QC report
(MS+HPLC), theoretical MW, purity ≥ 80%
Supporting Table 3: Protein provided by customers
Protein Name:
Molecular Weight:
Concentration (> 0.5 mg/ml required):
Quantity (>4 mg required):
Buffer:
Recommended storage condition:
Specify the tag if any:
QC standard: Purity ≥ 70%; quantity ≥4 mg; concentration ≥ 0.5 mg/ml.
For liquid samples, a document specifying the purity, concentration and volume is required.
For lyophilized samples, a document specifying the quantity is required.
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