Download The Central Dogma Dry Lab

The Central Dogma Dry Lab
Following is the base sequence of a gene on one strand of a DNA molecule (the SENSE
STRAND):
A A T G C C A G T G G T T C G C A C
1. What is the sequence of the complementary DNA strand (i.e. the NONSENSE
STRAND)?
2. What is the sequence of the mRNA transcribed from this gene?
3. Use the provided genetic code, to determine what amino acids will be in the protein
coded for by this gene.
4. If the fourth nucleotide in the original DNA strand was changed from G to C, what
sequence of mRNA would result?
i.e., the DNA sequence is now
A A T C C C A G T G G T T C G C A C
5. What sequence of amino acids would this code for?
6. If a G were added to the original DNA strand after the third nucleotide, what
sequence of mRNA would result?
i.e., the DNA sequence is now
A A T G G C C A G T G G T T C G C A C
7. What sequence of amino acids would this code for?
8. If the eighth nucleotide in the original DNA strand was changed from a G to a C,
what mRNA sequence would result?
i.e., the DNA sequence is now
A A T G C C A C T G G T T C G C A C
9. What sequence of amino acids would this code for? Why is this sequence so short?
U
First mRNA Base
A
Methionine (start)
G
valine
valine
valine
valine
Second mRNA Base
C
A
Serine
Tyrosine
Serine
Tyrosine
Serine
STOP
Serine
STOP
proline
Histidine
proline
Histidine
proline
Glutamine
proline
Glutamine
threonine
Aspragine
threonine
Asparagines
threonine
Lysine
threonine
Lysine
alanine
Aspartic acid
alanine
Asprartic acid
alanine
Glutamic acid
alanine
Glutamic acid
G
Cysteine
Cysteine
STOP
tryptophan
arginine
arginine
arginine
arginine
serine
serine
arginine
arginine
glycine
glycine
glycine
glycine
U
C
A
G
U
C
A
G
U
C
A
G
U
C
A
G
Third mRNA Base
C
U
phenylalanine
phenylalanine
leucine
leucine
leucine
leucine
leucine
leucine
isoleucine
isoleucine
isoleucine