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Transcript
No Pain, No Gain:
Understanding Muscle Pain
Jankowski Lab – Anesthesia/Pain Management
Jordan Lamb
Project Description
o Goal – Better understand molecular mechanisms that
lead to muscle pain after injury in order to determine
better treatments for musculoskeletal pain
o Muscle-specific injury model in mice
o Block blood flow in right forepaw by tying off brachial
artery (Ischemia)
o Remove blockage after several hours to allow blood flow
again (reperfusion)
o Causes changes in the muscle tissue and in gene
expression in afferent neurons that carry pain input
from muscle to CNS
Project Description Cont.
o Three types of procedures to study pain produced
after injury
o Behavior – guarding, grip strength, threshold they
respond to mechanical stimuli
o Electrophysiological recording – responsiveness to
mechanical, thermal, and chemical muscle stimulation
o Molecular Assays
o My role
o Focus on molecular assays
o Real-time PCR analysis
What? So What? Now What?
o Basic laboratory skills – pipetting, weighing chemicals,
preparing solutions, mouse handling, etc.
o Exposed to variety of different procedures
o RNA isolation, protein isolation, reverse transcription, PCR,
cell culture
o Assist with animal surgery
o About myself
o Like working in structured environment – following
procedure
o Gaining independence
o Asking for help
What? So What? Now What?
o Bettering understanding of
neuroscience
o Classroom and daily life
o Chance to explore other
science/medicine related
careers
o Perceptions on research
process
o A LOT of work
o More respect
o Prepared to continue research
this summer and in the future