* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Sickle Cell Anemia
Protein moonlighting wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Human genetic variation wikipedia , lookup
Epigenetics of neurodegenerative diseases wikipedia , lookup
Neuronal ceroid lipofuscinosis wikipedia , lookup
Gene therapy of the human retina wikipedia , lookup
Public health genomics wikipedia , lookup
History of genetic engineering wikipedia , lookup
Helitron (biology) wikipedia , lookup
Genome editing wikipedia , lookup
Genetic engineering wikipedia , lookup
Population genetics wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Genome (book) wikipedia , lookup
Frameshift mutation wikipedia , lookup
Genetic code wikipedia , lookup
Designer baby wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Sickle Cell Anemia Origins of sickle cell anemia and why it persists? 20 min Scott - What is a gene?- DNAi DNA – RNA – protein – link in animation of transc. and translation UWE -INTERACTIVE - show hemoglobin protein structure – go to NCBI – view protein structure Scott - What is a genetic disorder? Example of genetic disorder - Sickle Cell – Map of where disease is prevalent What causes the genetic disorder? Why does it persist? When did it originate? Scott - INTERACTIVE – 30 minutes Where is HB gene? NCBI - Human genome -use ncbi to pull out HB beta gene if time – intron/coding sequence get coding sequence GO TO sequence server give them HBS coding sequence intro start codon alignment what does this mean in terms of protein? Translate – GENEBOY – PROVIDE edited versions of sequence to put into geneboy Alignment – clustalW- Pasteur Uwe – 15 minutes Identify difference in proteins at amino acid level Compare 3D structure of HBB and HBS Show difference in side chains between E and V 15 minutes - Jennie - YGYH – what mutation causes in terms of protein polymerization – ISOLATE appropriate slides from “what causes sickle cell?” Movie from DNAi What does this do to the patient? Clinical context of disease – YGYH – interview with patient 45 minutes - Scott – what happens to a mutation within a population? Why is Sickle Cell maintained in a population. Simulation Server Genetic drift/ selection/ no selection Link to simulation server – Handout with exercises. Small population Large population Introduction to agriculture Sickle cell is present in other areas of the world. Overlap of where sickle cell is and where malaria is endemic Mutations can occur on different backgrounds – haplogroups Globin slide with chart – haplotyping. http://www.dpd.cdc.gov/dpdx/HTML/Malaria.asp?body=Frames/MR/Malaria/body_Malaria_page2.htm