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Transcript
Electrophoretic analysis of prion polymers Start SDS, % Start Polymers Sup35 2 0ï° 2 2 25ï° 37ï° 5 2 2 2 kDa 2 4200 (titin) 37ï° 42ï° 50ï° 70ï° 100 ï° 740 (nebulin) Monomers Sup35 205 (myosin) Sup35 monomers [psi-] Polymer SDS disrupts aggregates to polymers [PSI+] Stability of Sup35 polymers in the presence of SDS Analysis of polymers in agarose gel with SDS Kryndushkin et al., J. Biol. Chem., 2003 21
