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... genes [11]. Thus, the B-chain of ricin E shares its N-terminus with ricin D and the C-terminus with RCA120 as seen in Figure 1. Ricin E is produced in some of the R. communis ecotypes studied and has only been detected together with ricin D. Specific differentiation of the ricin isoforms from RCA120 ...
... genes [11]. Thus, the B-chain of ricin E shares its N-terminus with ricin D and the C-terminus with RCA120 as seen in Figure 1. Ricin E is produced in some of the R. communis ecotypes studied and has only been detected together with ricin D. Specific differentiation of the ricin isoforms from RCA120 ...
DETECTION OF IN BOVINE PREPUTIAL SCRAPINGS USING PCR AND CULTURE ASSAYS
... Furthermore, in summer the ambient temperatures in most parts of the country readily exceed 30 °C making it difficult to maintain the cold chain. With all these logistical difficulties it is not altogether surprising that, even under optimum laboratory conditions, low isolation rates are reported. C ...
... Furthermore, in summer the ambient temperatures in most parts of the country readily exceed 30 °C making it difficult to maintain the cold chain. With all these logistical difficulties it is not altogether surprising that, even under optimum laboratory conditions, low isolation rates are reported. C ...
Fulltext PDF - Indian Academy of Sciences
... levels of pre-existing antibody, serological testing using simple techniques and crude antigens (such as ELISA or indirect haemagglutination) is an effective diagnostic technique for intestinal as well as hepatic invasive amoebiasis (Pillai et al 1999). Results are much less clear-cut in disease-end ...
... levels of pre-existing antibody, serological testing using simple techniques and crude antigens (such as ELISA or indirect haemagglutination) is an effective diagnostic technique for intestinal as well as hepatic invasive amoebiasis (Pillai et al 1999). Results are much less clear-cut in disease-end ...
analytical applications of surface-modified fused silica capillaries
... and pumped through with relative ease, and due to their small dimensions allow fast mass transfer to and from the capillary walls as well as miniaturization of analytical systems. This dissertation describes two projects that utilize modification of fused silica capillary surfaces for specific analy ...
... and pumped through with relative ease, and due to their small dimensions allow fast mass transfer to and from the capillary walls as well as miniaturization of analytical systems. This dissertation describes two projects that utilize modification of fused silica capillary surfaces for specific analy ...
Lowering the Detection Limits of HIV-1 Viral Load Using Real
... sophisticated molecular techniques. Presently, the assay that attains maximal sensitivity and dynamic range of viral copy number is nucleic acid amplification of HIV-1 RNA in plasma: the Roche Amplicor HIV-1 Monitor Test (versions 1.0 and 1.5; Roche Molecular Systems, Basel, Switzerland). The linear ...
... sophisticated molecular techniques. Presently, the assay that attains maximal sensitivity and dynamic range of viral copy number is nucleic acid amplification of HIV-1 RNA in plasma: the Roche Amplicor HIV-1 Monitor Test (versions 1.0 and 1.5; Roche Molecular Systems, Basel, Switzerland). The linear ...
Laboratory Manual for the Diagnosis of Whooping
... Active primary immunization against B. pertussis infection is recommended, with three doses of a vaccine consisting of either a suspension of killed bacteria (whole-cell pertussis (wP) or acellular pertussis (aP) preparations that contain 1–5 different components of B. pertussis. These are usually g ...
... Active primary immunization against B. pertussis infection is recommended, with three doses of a vaccine consisting of either a suspension of killed bacteria (whole-cell pertussis (wP) or acellular pertussis (aP) preparations that contain 1–5 different components of B. pertussis. These are usually g ...
Culture Media for Detection of Acinetobacter baumannii Selective
... A. baumannii the most clinically relevant of the bacteria tested, have a particular propensity for nosocomial transmission, due in part to their sustained survival on environmental surfaces as well as their multidrug resistance. The prevalence of infection with A. baumannii has increased significant ...
... A. baumannii the most clinically relevant of the bacteria tested, have a particular propensity for nosocomial transmission, due in part to their sustained survival on environmental surfaces as well as their multidrug resistance. The prevalence of infection with A. baumannii has increased significant ...
Tag-ItTM Mutation Detection Kit for CFTR 70+6
... Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
... Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
Chapter 2.2.3.
... the presence of IHHN in farmed prawns in Australia was reported to the OIE in 2008. As discussed in Section 2.1.1, IHHNV-related sequences have been found inserted into the genome of P. monodon from East Africa, Australia, and the western Indo-Pacific region (Tang & Lightner, 2006; Tang et al., 2007 ...
... the presence of IHHN in farmed prawns in Australia was reported to the OIE in 2008. As discussed in Section 2.1.1, IHHNV-related sequences have been found inserted into the genome of P. monodon from East Africa, Australia, and the western Indo-Pacific region (Tang & Lightner, 2006; Tang et al., 2007 ...
Polymerase Chain Reaction In Ophthalmology
... the basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khurana in 19712. The PCR is superior in terms of sensitivity, specificity and rapidity of other diagnostic tests in the armamentarium. The presence of DNA or RNA of the pathogen can directly be ...
... the basic principle of replicating a piece of DNA using two primers had already been described by Gobind Khurana in 19712. The PCR is superior in terms of sensitivity, specificity and rapidity of other diagnostic tests in the armamentarium. The presence of DNA or RNA of the pathogen can directly be ...
The diagnostic role of Saliva — A Review.
... HIV Antibody to HIV in whole saliva of infected individuals was detected by ELISA and Western blot assay, correlated with serum antibody levels (15). Salivary IgA levels to HIV decline as infected patients become symptomatic. It was suggested that detection of IgA antibody to HIV in saliva may, ther ...
... HIV Antibody to HIV in whole saliva of infected individuals was detected by ELISA and Western blot assay, correlated with serum antibody levels (15). Salivary IgA levels to HIV decline as infected patients become symptomatic. It was suggested that detection of IgA antibody to HIV in saliva may, ther ...
Novel tests for diagnosing tuberculous pleural effusion: what works and what REVIEW
... ADA activity measurement is simple and inexpensive to perform and does not require special equipment, making it an attractive option for settings with high TB incidence and limited resources. In contrast, measurement of IFN-c is expensive, estimated to be as much as the cost of treatment for six TB ...
... ADA activity measurement is simple and inexpensive to perform and does not require special equipment, making it an attractive option for settings with high TB incidence and limited resources. In contrast, measurement of IFN-c is expensive, estimated to be as much as the cost of treatment for six TB ...
Performance Characteristics of Three Assays for the Therapeutic
... analyzers: (i) The Abbott TDx/FLx Methotrexate II assay on the TDx/FLx analyzer as the reference method. This assay utilizes a fluorescence polarization immunoassay technique. (ii) Siemens Syva® Emit® Methotrexate assay on the Vitros 5,1 Fusion from Ortho Clinical Diagnostics. This assay utilizes a ...
... analyzers: (i) The Abbott TDx/FLx Methotrexate II assay on the TDx/FLx analyzer as the reference method. This assay utilizes a fluorescence polarization immunoassay technique. (ii) Siemens Syva® Emit® Methotrexate assay on the Vitros 5,1 Fusion from Ortho Clinical Diagnostics. This assay utilizes a ...
The Department of Electrical and Computer Engineering
... Figure 2.2 Illustration of protein occupying the surface of a carbon nanotube in helical manner (Balavoine et al.,1999 [50]). ......................................................................................................... 13 Figure 2.3 Demonstration of the conjugation process of proteins t ...
... Figure 2.2 Illustration of protein occupying the surface of a carbon nanotube in helical manner (Balavoine et al.,1999 [50]). ......................................................................................................... 13 Figure 2.3 Demonstration of the conjugation process of proteins t ...
The Use of Computational Intelligence in Intrusion Detection Systems
... As sharing the same root with the DARPA dataset, the KDD99 dataset inherits the above limitations. In addition, the empirical study conducted by Sabhnani et al. [239] stated that “KDD training and test data subsets represent dissimilar target hypotheses for U2R and R2L attack categories”. According ...
... As sharing the same root with the DARPA dataset, the KDD99 dataset inherits the above limitations. In addition, the empirical study conducted by Sabhnani et al. [239] stated that “KDD training and test data subsets represent dissimilar target hypotheses for U2R and R2L attack categories”. According ...
URINE is USEFUL! - Stark, William S.
... • Proteinuria may be the first indication of renovascular, glomerular, or tubulointerstitial renal disease, or may represent the flow of abnormal proteins in the urine in conditions such as multiple myeloma. • Transient proteinuria can be common, especially in the pediatric population and resolves ...
... • Proteinuria may be the first indication of renovascular, glomerular, or tubulointerstitial renal disease, or may represent the flow of abnormal proteins in the urine in conditions such as multiple myeloma. • Transient proteinuria can be common, especially in the pediatric population and resolves ...
GenScript TissueDirect Multiplex PCR System Protocol
... Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. 2 The nucleotide sequence of mouse laminin A chain has an open reading frame enc ...
... Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. 2 The nucleotide sequence of mouse laminin A chain has an open reading frame enc ...
What is the risk of chronic wasting disease being introduced into
... exposed to ruminant animal feed containing deer protein from North America is considered negligible but with associated uncertainty. The probability of a (wild) deer being exposed to CWD infected deer protein in non-ruminant feed is considered to be greater than negligible but uncertain. The pathway ...
... exposed to ruminant animal feed containing deer protein from North America is considered negligible but with associated uncertainty. The probability of a (wild) deer being exposed to CWD infected deer protein in non-ruminant feed is considered to be greater than negligible but uncertain. The pathway ...
HBsAg II - Roche Canada
... Immunoassay for the in vitro qualitative determination of hepatitis B surface antigen (HBsAg) in human serum and plasma. This assay is indicated as an aid in the diagnosis of infection with HBV. This assay is also indicated as a donor screening test to detect HBV in serum or plasma specimens from in ...
... Immunoassay for the in vitro qualitative determination of hepatitis B surface antigen (HBsAg) in human serum and plasma. This assay is indicated as an aid in the diagnosis of infection with HBV. This assay is also indicated as a donor screening test to detect HBV in serum or plasma specimens from in ...
The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA
... The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA assays as measured on the Qubit Fluorometer Glycogen (a branched-chain carbohydrate; Cat. No. 10814010) and Invitrogen™ GlycoBlue™ Coprecipitant (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to fa ...
... The use of glycogen and GlycoBlue reagent in Qubit DNA and RNA assays as measured on the Qubit Fluorometer Glycogen (a branched-chain carbohydrate; Cat. No. 10814010) and Invitrogen™ GlycoBlue™ Coprecipitant (glycogen covalently linked to a blue dye; Cat. No. AM9515) are reagents commonly used to fa ...
Promega Notes 39: Stabilized TMB Substrate for Horseradish
... Immunodetection on Western blots using TMB Stabilized Substrate and CN was performed to compare the detection sensitivities obtainable with these color development substrates. Samples of serially diluted beta-galactosidase (Worthington) were run on an 8% SDS-polyacrylamide minigel and electroblotted ...
... Immunodetection on Western blots using TMB Stabilized Substrate and CN was performed to compare the detection sensitivities obtainable with these color development substrates. Samples of serially diluted beta-galactosidase (Worthington) were run on an 8% SDS-polyacrylamide minigel and electroblotted ...
NS1 antigen ELISA
... Dengue fever caused by dengue virus (DENV), a member of Flaviviridae leads to large global disease burden. Detection of immunoglobulin M (IgM) and nucleic acid to DENV, and virus isolation have been used for laboratory diagnostic assays for dengue. Nonstructural 1 (NS1) antigen which releases from D ...
... Dengue fever caused by dengue virus (DENV), a member of Flaviviridae leads to large global disease burden. Detection of immunoglobulin M (IgM) and nucleic acid to DENV, and virus isolation have been used for laboratory diagnostic assays for dengue. Nonstructural 1 (NS1) antigen which releases from D ...
Recent Development in Optical Fiber Biosensors
... Keywords: Optical fiber, biosensor, applications, review ...
... Keywords: Optical fiber, biosensor, applications, review ...
MycoplasMa Quality control
... sample preparation and validation of the system to ensure equivalency or superiority to conventional test methods. To meet this need, ATCC has developed titered mycoplasma reference standards for comparing PCR and culture-based detection methods, and quantitative mycoplasma DNA certified reference m ...
... sample preparation and validation of the system to ensure equivalency or superiority to conventional test methods. To meet this need, ATCC has developed titered mycoplasma reference standards for comparing PCR and culture-based detection methods, and quantitative mycoplasma DNA certified reference m ...
BILE ACIDS - Liquid Chromatography
... Fluorescence detection A method to increase sensitivity in determination of bile acids is Suorescence labelling and Suorescence detection. Two different systems to generate Suorescent bile acid derivatives are commonly used. A relatively old method to quantify total bile acid concentrations is enzym ...
... Fluorescence detection A method to increase sensitivity in determination of bile acids is Suorescence labelling and Suorescence detection. Two different systems to generate Suorescent bile acid derivatives are commonly used. A relatively old method to quantify total bile acid concentrations is enzym ...