Applications of Real -Time PCR
... for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both q ...
... for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real Time PCR has emerged as a technological innovation and is playing an ever-increasing role in clinical diagnostics and research laboratories. Due to its capacity to generate both q ...
Antibodies to Human, Animal, and Plant Pathogens
... KPL supports research in zoonotic pathogens that ultimately infect humans with antibodies against Borrelia burgdorferi and Yersinia. B. burgdoferi is transmitted by ticks and causes Lyme disease in humans. Yersinia can be transmitted by rodents and is responsible for several high-mortality epidemics ...
... KPL supports research in zoonotic pathogens that ultimately infect humans with antibodies against Borrelia burgdorferi and Yersinia. B. burgdoferi is transmitted by ticks and causes Lyme disease in humans. Yersinia can be transmitted by rodents and is responsible for several high-mortality epidemics ...
Lesson 24. lmmuno- chemical techniques
... the more advanced the immune system. The immune system of mammals has evolved over a million years; it provides an incredible protection system capable of responding to infective challenges that arise in the body. Immunity in our body is monitored or controlled by specific cells from stem cells in b ...
... the more advanced the immune system. The immune system of mammals has evolved over a million years; it provides an incredible protection system capable of responding to infective challenges that arise in the body. Immunity in our body is monitored or controlled by specific cells from stem cells in b ...
Mycoplasma pneumoniae IgG, IgM
... Optimal antigen selection • LIAISON® Mycoplasma pneumoniae IgG uses recombinant antigens against the 170-kDa P1 adhesion protein of M. pneumoniae • LIAISON® Mycoplasma pneumoniae IgM, in addition to the P1 antigen, incorporates whole-cell lysate ...
... Optimal antigen selection • LIAISON® Mycoplasma pneumoniae IgG uses recombinant antigens against the 170-kDa P1 adhesion protein of M. pneumoniae • LIAISON® Mycoplasma pneumoniae IgM, in addition to the P1 antigen, incorporates whole-cell lysate ...
Mycoplasma pneumoniae IgG, IgM
... Optimal antigen selection • LIAISON® Mycoplasma pneumoniae IgG uses recombinant antigens against the 170-kDa P1 adhesion protein of M. pneumoniae • LIAISON® Mycoplasma pneumoniae IgM, in addition to the P1 antigen, incorporates whole-cell lysate ...
... Optimal antigen selection • LIAISON® Mycoplasma pneumoniae IgG uses recombinant antigens against the 170-kDa P1 adhesion protein of M. pneumoniae • LIAISON® Mycoplasma pneumoniae IgM, in addition to the P1 antigen, incorporates whole-cell lysate ...
Fluorescent Probe Studies of Proteins
... On the day of the phosphorylase kinase assays, dilute phosphorylase b into the stock buffer for a protein concentration of 5.2 mg/ml. Dithiothreitol (0.2 M stock) is then added to a final concentration of 2 mM. Important: Add a small amount of charcoal (~0.5 mg/ml; Norit A) to the solution in order ...
... On the day of the phosphorylase kinase assays, dilute phosphorylase b into the stock buffer for a protein concentration of 5.2 mg/ml. Dithiothreitol (0.2 M stock) is then added to a final concentration of 2 mM. Important: Add a small amount of charcoal (~0.5 mg/ml; Norit A) to the solution in order ...
Pyruvate Assay Kit - Cell Biolabs, Inc.
... alanine. Pyruvate can be converted into ethanol or lactic acid through the process of fermentation. Finally, pyruvate is a key component in providing energy to cells through the citric acid cycle under oxygen rich conditions (aerobic respiration), and can also lead to production of lactate in anaero ...
... alanine. Pyruvate can be converted into ethanol or lactic acid through the process of fermentation. Finally, pyruvate is a key component in providing energy to cells through the citric acid cycle under oxygen rich conditions (aerobic respiration), and can also lead to production of lactate in anaero ...
View Full Text-PDF
... were from blood followed by sputum, urine and exudates. The worldwide emergence of multi-drug resistant bacterial strains is a growing concern which are usually found in those hospitals where antibiotic use is frequent and the patients are in critical condition (Shahanara Begum et al., 2013). Broad ...
... were from blood followed by sputum, urine and exudates. The worldwide emergence of multi-drug resistant bacterial strains is a growing concern which are usually found in those hospitals where antibiotic use is frequent and the patients are in critical condition (Shahanara Begum et al., 2013). Broad ...
Comparative Evaluation of the Limulus Assay and the Direct Gram
... platinum loop is a suitable alternate method requiring less time and equipment. 7 However, both methods still require skill, time, and equipment not readily available in all office or clinic settings. Consequently, several miniaturized culture devices and chemical tests have been proposed as practic ...
... platinum loop is a suitable alternate method requiring less time and equipment. 7 However, both methods still require skill, time, and equipment not readily available in all office or clinic settings. Consequently, several miniaturized culture devices and chemical tests have been proposed as practic ...
Intended Use
... For the quantitative determination of Aspartate Aminotransferase (AST) in human serum on Hitachi analyzers. ...
... For the quantitative determination of Aspartate Aminotransferase (AST) in human serum on Hitachi analyzers. ...
trypsin-induced hemagglutination assay for the detection of
... addition to THA, trypsin may be used for the production of stable and high-titer IBV antigen for hemagglutination inhibition (HI) assay as well. Trypsin was shown to be a strong and specific inducer of HA activity for IBV in embryos inoculated with IBV alone and when combined with several other avia ...
... addition to THA, trypsin may be used for the production of stable and high-titer IBV antigen for hemagglutination inhibition (HI) assay as well. Trypsin was shown to be a strong and specific inducer of HA activity for IBV in embryos inoculated with IBV alone and when combined with several other avia ...
[BIO24] Detection of infectious bursal disease virus using SYBR
... detect vaccine and wild type IBDV strains in infected chickens. Although, these method was able to differentiate different IBDV strains, they are not preferred to be tested on large number of clinical samples. The present study, offers an alternative format assay in detecting and differentiate of ve ...
... detect vaccine and wild type IBDV strains in infected chickens. Although, these method was able to differentiate different IBDV strains, they are not preferred to be tested on large number of clinical samples. The present study, offers an alternative format assay in detecting and differentiate of ve ...
Comparative analysis of different TaqMan real-time RT
... analysis (Aggarwal and Krawczynski 2000; Smith 2001). There is increasing evidence that animals (such as pigs) could serve as reservoir for HEV (Meng et al. 1997, 1998, 1999, 2002; Yoo et al. 2001; Renou et al. 2007). Swine HEV isolates have been identified now in many countries worldwide including ...
... analysis (Aggarwal and Krawczynski 2000; Smith 2001). There is increasing evidence that animals (such as pigs) could serve as reservoir for HEV (Meng et al. 1997, 1998, 1999, 2002; Yoo et al. 2001; Renou et al. 2007). Swine HEV isolates have been identified now in many countries worldwide including ...
Detection of Avian Influenza-DNA Hybridization
... they can be used for real-time monitoring and sensitive and quantitative detection [4]. In particular, label-free measurements enable SPR technology to be used for direct detection of binding events between a target analyte and its corresponding receptor. This method is also applicable in situ becau ...
... they can be used for real-time monitoring and sensitive and quantitative detection [4]. In particular, label-free measurements enable SPR technology to be used for direct detection of binding events between a target analyte and its corresponding receptor. This method is also applicable in situ becau ...
Prying Into Prions - Colorado Parks and Wildlife
... Chronic Wasting Disease (CWD) has been recognized in free-ranging deer in Colorado since 1985, but how long it has been present or how it arose are unknown. Unlike all infectious diseases known at the time, the pathogen for transmissible spongiform encephalopathies or TSEs was identified as a protei ...
... Chronic Wasting Disease (CWD) has been recognized in free-ranging deer in Colorado since 1985, but how long it has been present or how it arose are unknown. Unlike all infectious diseases known at the time, the pathogen for transmissible spongiform encephalopathies or TSEs was identified as a protei ...
Quantumdotsbased fluoroimmunoassay for the rapid and sensitive
... antibody. Based on these QD–antibody conjugates, a novel sandwich FL-linked immunosorbent assay (sFLISA) was developed for H5N1 viral antigen detection. The sFLISA allowed for H5N1 viral antigen determination in a linear range of 8.0 ¥ 10-3 to 5.1 ¥ 10-1 mg mL-1 with the limit of detection (LOD) of ...
... antibody. Based on these QD–antibody conjugates, a novel sandwich FL-linked immunosorbent assay (sFLISA) was developed for H5N1 viral antigen detection. The sFLISA allowed for H5N1 viral antigen determination in a linear range of 8.0 ¥ 10-3 to 5.1 ¥ 10-1 mg mL-1 with the limit of detection (LOD) of ...
PCR and the detection of pathogens in W and WW
... viruses directly in water and wastewaters. Green and Lewis (1995) developed a method for extracting and ampli®cation of enteroviruses from wastewater which had a sensitivity of 0.02±0.2 plaque forming units (pfu) (equivalent to approximately 1 pfu/L). Wyn-Jones et al. (1995) compared PCR with cell c ...
... viruses directly in water and wastewaters. Green and Lewis (1995) developed a method for extracting and ampli®cation of enteroviruses from wastewater which had a sensitivity of 0.02±0.2 plaque forming units (pfu) (equivalent to approximately 1 pfu/L). Wyn-Jones et al. (1995) compared PCR with cell c ...
Serodiagnosis of Peste des Petits Ruminants Virus
... Fig. 2. A schematic illustration of the PPR virus structure. The arrangement of the viral proteins is shown in the structure (A). The names of the viral proteins present in the virus structure are shown in the box (B). The viral genome organization and arrangement of the different viral proteins in ...
... Fig. 2. A schematic illustration of the PPR virus structure. The arrangement of the viral proteins is shown in the structure (A). The names of the viral proteins present in the virus structure are shown in the box (B). The viral genome organization and arrangement of the different viral proteins in ...
Epidemiology 231 - UCLA School of Public Health
... Bacteria reproduce every 30 minutes. For them, a millennium is compressed into a fortnight. They are fleet afoot, and the pace of our research must keep up with them, or they will overtake us. Microbes were here on earth 2 billion years before humans arrived, learning every trick for survival, and i ...
... Bacteria reproduce every 30 minutes. For them, a millennium is compressed into a fortnight. They are fleet afoot, and the pace of our research must keep up with them, or they will overtake us. Microbes were here on earth 2 billion years before humans arrived, learning every trick for survival, and i ...
Detection of Salmonella spp. Presence in Food
... steps to control safety and quality of food. Development of new, fast, and reliable identification methods for biological threats are necessary to meet the safety standards of food products and risk management. Salmonella spp., a marker of food products safety, is widely distributed foodborne pathog ...
... steps to control safety and quality of food. Development of new, fast, and reliable identification methods for biological threats are necessary to meet the safety standards of food products and risk management. Salmonella spp., a marker of food products safety, is widely distributed foodborne pathog ...
Multicentre laboratory evaluation of Brugia Rapid dipstick test for
... 100% (Table 2). The latter two filarial species are not coendemic with B. malayi, thus, operationally they need not be included in the determination of the test’s specificity. In the previous evaluation study, Brugia Rapid was reported to show 96.6% sensitivity and 98.9% specificity. These values ar ...
... 100% (Table 2). The latter two filarial species are not coendemic with B. malayi, thus, operationally they need not be included in the determination of the test’s specificity. In the previous evaluation study, Brugia Rapid was reported to show 96.6% sensitivity and 98.9% specificity. These values ar ...
ammonia assay - Sekisui Diagnostics
... produced from dietary and amino acid metabolism. Blood ammonia measurements have been used in the diagnosis of coma associated with hepatic dysfunction caused by cirrhosis and neoplasms. The measurement of ammonia is very useful in the diagnosis and prognosis of Reye's Syndrome. The assay of blood a ...
... produced from dietary and amino acid metabolism. Blood ammonia measurements have been used in the diagnosis of coma associated with hepatic dysfunction caused by cirrhosis and neoplasms. The measurement of ammonia is very useful in the diagnosis and prognosis of Reye's Syndrome. The assay of blood a ...
Microbiology Laboratory Users Manual
... The collection of correct microbiology specimens, taken at the appropriate time is essential in the diagnosis of infection and disease. Specimens must be sent to the Laboratory in a timely fashion (see Section 7 for specimen transport procedure). ...
... The collection of correct microbiology specimens, taken at the appropriate time is essential in the diagnosis of infection and disease. Specimens must be sent to the Laboratory in a timely fashion (see Section 7 for specimen transport procedure). ...