Detection of bacteria, fungi, and viruses by a real
... assay and in 9 of 76 (11.8%) by blood culture. In 6 blood culture-positive samples, real-time PCR assay detected the same type of bacteria. No fungus was detected both real-time PCR assay and blood culture. Viruses were identified in 6 of 76 FNE (7.9%). During antibiotic therapy, all samples were n ...
... assay and in 9 of 76 (11.8%) by blood culture. In 6 blood culture-positive samples, real-time PCR assay detected the same type of bacteria. No fungus was detected both real-time PCR assay and blood culture. Viruses were identified in 6 of 76 FNE (7.9%). During antibiotic therapy, all samples were n ...
Enterobacteriaceae GNRs
... • Screen: K-B with oxacillin on MHA w/5% sheep blood • < 20 mm zone = presumptive evidence of resistance to penicillin ...
... • Screen: K-B with oxacillin on MHA w/5% sheep blood • < 20 mm zone = presumptive evidence of resistance to penicillin ...
Critically discuss the methods that are in routine use for the
... binding is usually measured. However, dye binding methods are less specific than nephelometry or turbidimetry and are inaccurate if the overall serum protein pattern is abnormal, such as in multiple myeloma. The BCG and BCP methods both have advantages and disadvantages, with neither being clearly p ...
... binding is usually measured. However, dye binding methods are less specific than nephelometry or turbidimetry and are inaccurate if the overall serum protein pattern is abnormal, such as in multiple myeloma. The BCG and BCP methods both have advantages and disadvantages, with neither being clearly p ...
Limitations of PCR-Based Assays Performed on Formalin
... limited success, Wiland et al8 emphasize the importance of continuing to develop molecular assays for gastrointestinal pathogens that are suitable for use in fixed, paraffin-embedded tissue. Part of our mission as pathologists, laboratorians, and consultants is to educate our colleagues and the publ ...
... limited success, Wiland et al8 emphasize the importance of continuing to develop molecular assays for gastrointestinal pathogens that are suitable for use in fixed, paraffin-embedded tissue. Part of our mission as pathologists, laboratorians, and consultants is to educate our colleagues and the publ ...
as a PDF
... small volumes of plasma and whole blood has been developed. Based on isotope-dilution tandem mass spectrometry, the method takes only a few minutes to perform and requires minimal sample preparation. The accurate assay of both phenylalanine and tyrosine in dried blood spots used for neonatal screeni ...
... small volumes of plasma and whole blood has been developed. Based on isotope-dilution tandem mass spectrometry, the method takes only a few minutes to perform and requires minimal sample preparation. The accurate assay of both phenylalanine and tyrosine in dried blood spots used for neonatal screeni ...
infectious pustular vulvovaginitis
... For virus isolation, bovine cells of various origins [various cell cultures] can be used. Primary or secondary bovine kidney, lung or testis cells, cell strains derived from bovine fetal lung, turbinate or trachea, and established cell lines, such as the Madin–Darby bovine kidney cell line (MDBK), a ...
... For virus isolation, bovine cells of various origins [various cell cultures] can be used. Primary or secondary bovine kidney, lung or testis cells, cell strains derived from bovine fetal lung, turbinate or trachea, and established cell lines, such as the Madin–Darby bovine kidney cell line (MDBK), a ...
Sensitivity of alpha-1 antitrypsin level for inherited deficiency
... Diagnosis of this genetic condition is confirmed by genetic verification of pathology, but for screening purposes quantitative methods can be useful. The aim of our study was to evaluate sensitivity and specificity of quantitative methods for alpha-1 antitrypsin deficiency detection. Methods. Serum ...
... Diagnosis of this genetic condition is confirmed by genetic verification of pathology, but for screening purposes quantitative methods can be useful. The aim of our study was to evaluate sensitivity and specificity of quantitative methods for alpha-1 antitrypsin deficiency detection. Methods. Serum ...
IDEXX Canine And Feline Diarrhea RealPCR Panels
... 16. Pedersen NC. An overview of feline enteric coronavirus and infectious peritonitis virus infections. Feline Practice. 1995; 23(3):7–20. 17. Lutz H. Biology of feline coronavirus and its control. Proceedings from: 28th World Congress of the World Small Animal Veterinary Association; October 24–27, ...
... 16. Pedersen NC. An overview of feline enteric coronavirus and infectious peritonitis virus infections. Feline Practice. 1995; 23(3):7–20. 17. Lutz H. Biology of feline coronavirus and its control. Proceedings from: 28th World Congress of the World Small Animal Veterinary Association; October 24–27, ...
Chronic Wasting Disease in Deer and Elk in Arkansas
... Research has demonstrated that CWD can be spread directly through animal-to-animal contact and indirectly through prion-contaminated soil or other surfaces. Perhaps the most common mode of trans mission from animal-to-animal is saliva, urine and feces. People may have aided the spread of the diseas ...
... Research has demonstrated that CWD can be spread directly through animal-to-animal contact and indirectly through prion-contaminated soil or other surfaces. Perhaps the most common mode of trans mission from animal-to-animal is saliva, urine and feces. People may have aided the spread of the diseas ...
Diagnosis of bacterial vaginosis by a new multiplex peptide
... and user dependent (Sha et al., 2005). Furthermore, due to its low specificity, the Nugent method also considers intermediate microflora whenever the final score is between 4 and 6. Although both methodologies are easy and fast to perform, they do not provide a robust diagnosis of BV. When combined, ...
... and user dependent (Sha et al., 2005). Furthermore, due to its low specificity, the Nugent method also considers intermediate microflora whenever the final score is between 4 and 6. Although both methodologies are easy and fast to perform, they do not provide a robust diagnosis of BV. When combined, ...
Chronic Wasting Disease (CWD):Layout 1
... Chronic Wasting Disease (CWD) is a contagious neurological disease affecting deer and elk. It causes a characteristic sponge-like degeneration of the brains of infected animals, resulting in emaciation, abnormal behavior, loss of bodily functions, and death. CWD belongs to a group of diseases known ...
... Chronic Wasting Disease (CWD) is a contagious neurological disease affecting deer and elk. It causes a characteristic sponge-like degeneration of the brains of infected animals, resulting in emaciation, abnormal behavior, loss of bodily functions, and death. CWD belongs to a group of diseases known ...
PCR detection of infectious diseases
... infectious diseases: detect the organism or detect antibodies against the organism. Most frequently, they detect infectious agents in biological specimens by culture, cytology, fecal examination, histopathology, immunological techniques and nucleic acid amplification techniques. The polymerase chain ...
... infectious diseases: detect the organism or detect antibodies against the organism. Most frequently, they detect infectious agents in biological specimens by culture, cytology, fecal examination, histopathology, immunological techniques and nucleic acid amplification techniques. The polymerase chain ...
Thyretain
... The TSI Reporter Controls are supplied at working strength. Any dilution of these reagents will decrease sensitivity. Reagents should be used prior to their expiration date. Each multi-well plate should be used only once. Do not re-use previously assayed plate. Microbial contamination of the CHO Mc4 ...
... The TSI Reporter Controls are supplied at working strength. Any dilution of these reagents will decrease sensitivity. Reagents should be used prior to their expiration date. Each multi-well plate should be used only once. Do not re-use previously assayed plate. Microbial contamination of the CHO Mc4 ...
Diagnosis of Legionella Infection
... Serological testing for Legionella infection is a valuable epidemiological tool but has little impact on clinical decision making because of the time delay before a result is available. The antibodies produced in response to infection are generally a mixture of IgA, IgM, and IgG, and tests should de ...
... Serological testing for Legionella infection is a valuable epidemiological tool but has little impact on clinical decision making because of the time delay before a result is available. The antibodies produced in response to infection are generally a mixture of IgA, IgM, and IgG, and tests should de ...
The reference range for serum creatinine is as follows
... same procedure (Sclavo) at 30 °C in 73 samples ranging in concentration from 0.6 mg/dL to 5.77 mg/dL, the correlation coefficient was 0.997 and the regression equation was y = 1.022x + 0.010. Precision: Assays were run, following the procedure described above, on two serum pools. Results ...
... same procedure (Sclavo) at 30 °C in 73 samples ranging in concentration from 0.6 mg/dL to 5.77 mg/dL, the correlation coefficient was 0.997 and the regression equation was y = 1.022x + 0.010. Precision: Assays were run, following the procedure described above, on two serum pools. Results ...
UC Davis Medical Center, Sacramento, CA
... o Be able to list the bacterial, fungal and mycobacterial organisms for which routine bacterial blood cultures may not or are not sufficient for detection. o Be able to list alternative non-culture based tests available for the diagnosis of common and unusual blood stream infections. o Be able to di ...
... o Be able to list the bacterial, fungal and mycobacterial organisms for which routine bacterial blood cultures may not or are not sufficient for detection. o Be able to list alternative non-culture based tests available for the diagnosis of common and unusual blood stream infections. o Be able to di ...
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)
... infection, while as IgG positive response suggests a previous or chronic infection. To avoid false negative result due to undetectable level of AFB in the smear (paucibacillary), or poor quality of specimen collection, staining and microscopy, culture technique for AFB used as a Gold standard for th ...
... infection, while as IgG positive response suggests a previous or chronic infection. To avoid false negative result due to undetectable level of AFB in the smear (paucibacillary), or poor quality of specimen collection, staining and microscopy, culture technique for AFB used as a Gold standard for th ...
Rapid Detection of Klebsiella Pneumoniae by Capsular Polysaccharide Antigen
... results may be available only after 48 hours. Even then accurate identification at species level of the genus may not be possible in all cases. In clinical situations, the severity of infections does not warrant such a long period of investigation for an appropriate treatment to be started. Speed of ...
... results may be available only after 48 hours. Even then accurate identification at species level of the genus may not be possible in all cases. In clinical situations, the severity of infections does not warrant such a long period of investigation for an appropriate treatment to be started. Speed of ...
6 Brief Resume of the intended work 6.1 Need for the study Dengue
... virus, its nucleic acid and circulating viral antigen can be detected.4 Among the nonstructural proteins, NS1 is a highly conserved glycoprotein which appears essential for virus replication, although no precise function has yet been assigned to it. During acute dengue virus infection, NS1 is found ...
... virus, its nucleic acid and circulating viral antigen can be detected.4 Among the nonstructural proteins, NS1 is a highly conserved glycoprotein which appears essential for virus replication, although no precise function has yet been assigned to it. During acute dengue virus infection, NS1 is found ...
Recent molecular biology methods for foulbrood and
... AFB. Moreover, Nosema apis has been known for years to be the causative agent of nosemosis, but recently another microsporidian was found in Apis mellifera. Nosema ceranae infection does not induce any of the overt clinical signs observed after N. apis infection, such as diarrhoea (1). Optical micro ...
... AFB. Moreover, Nosema apis has been known for years to be the causative agent of nosemosis, but recently another microsporidian was found in Apis mellifera. Nosema ceranae infection does not induce any of the overt clinical signs observed after N. apis infection, such as diarrhoea (1). Optical micro ...
results - Saudi Medical Journal
... enzymes of Ambler class A. The most significance ESBL genes are SHV-1, TEM-1 and TEM-2 which are generated due to mutations, and broaden the substrate specificity against broad spectrum cephalosporin’s, commonly to ceftazidime, cefotaxime and ceftriaxone. These βlactamases, show evidence of a broad ...
... enzymes of Ambler class A. The most significance ESBL genes are SHV-1, TEM-1 and TEM-2 which are generated due to mutations, and broaden the substrate specificity against broad spectrum cephalosporin’s, commonly to ceftazidime, cefotaxime and ceftriaxone. These βlactamases, show evidence of a broad ...
Intestinal parasite antigen testing-the next generation of fecal
... researching this occurrence found that 31.5% Toxocara‑positive canine fecal specimens were in fact T. cati eggs.5 Another common problem concerns the varying density of the different eggs, which makes it difficult for a clinician to select the ideal fecal flotation solution to ensure adequate recove ...
... researching this occurrence found that 31.5% Toxocara‑positive canine fecal specimens were in fact T. cati eggs.5 Another common problem concerns the varying density of the different eggs, which makes it difficult for a clinician to select the ideal fecal flotation solution to ensure adequate recove ...
The microbiological diagnosis of tuberculous meningitis: results of
... We aimed to provide data on the diagnosis of tuberculous meningitis (TBM) in this largest case series ever reported. The Haydarpasa-1 study involved patients with microbiologically confirmed TBM in Albania, Croatia, Denmark, Egypt, France, Hungary, Iraq, Italy, Macedonia, Romania, Serbia, Slovenia, ...
... We aimed to provide data on the diagnosis of tuberculous meningitis (TBM) in this largest case series ever reported. The Haydarpasa-1 study involved patients with microbiologically confirmed TBM in Albania, Croatia, Denmark, Egypt, France, Hungary, Iraq, Italy, Macedonia, Romania, Serbia, Slovenia, ...
DiaEasy - BioVision
... Biovision’s DiaEasy™ dialyzer (1 kDa MWCO) can be used primarily for dialysis or buffer exchanges of sample volumes upto 15 ml. They can also be used for several other purposes as shown below. The DiaEasy™ tube’s membrane is ultra-clean, sulfur and heavy metal free and EDTA treated which makes it su ...
... Biovision’s DiaEasy™ dialyzer (1 kDa MWCO) can be used primarily for dialysis or buffer exchanges of sample volumes upto 15 ml. They can also be used for several other purposes as shown below. The DiaEasy™ tube’s membrane is ultra-clean, sulfur and heavy metal free and EDTA treated which makes it su ...
Development of Field-Based Real-Time Reverse Transcription
... of disease worldwide with geographic distributions that are divided into the Old and New World viruses. Old World alphaviruses generally cause rash, arthritis, and arthralgia in affected humans and are distributed throughout Africa, Asia, and Europe. New World alphaviruses may cause an acute febrile ...
... of disease worldwide with geographic distributions that are divided into the Old and New World viruses. Old World alphaviruses generally cause rash, arthritis, and arthralgia in affected humans and are distributed throughout Africa, Asia, and Europe. New World alphaviruses may cause an acute febrile ...