Download Ch. 9

Chapter 9
Cellular Respiration:
Harvesting Chemical Energy
PowerPoint Lectures for
Biology, Seventh Edition
Neil Campbell and Jane Reece
Lectures by Chris Romero
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Overview: Life Is Work
• Living cells require energy from outside sources
• Some animals, such as the giant panda, obtain
energy by eating plants; others feed on organisms
that eat plants
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Energy flows into an ecosystem as sunlight and
leaves as heat
• Photosynthesis generates oxygen and organic
molecules, which are used in cellular respiration
• Cells use chemical energy stored in organic
molecules to regenerate ATP, which powers work
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-2
Light
energy
ECOSYSTEM
Photosynthesis
in chloroplasts
Organic + O
molecules 2
CO2 + H2O
Cellular respiration
in mitochondria
ATP
powers most cellular work
Heat
energy
Concept 9.1: Catabolic pathways yield energy by
oxidizing organic fuels
• Several processes are central to cellular
respiration and related pathways
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Catabolic Pathways and Production of ATP
• The breakdown of organic molecules is exergonic
• Fermentation is a partial degradation of sugars
that occurs without oxygen
• Cellular respiration consumes oxygen and organic
molecules and yields ATP
• Although carbohydrates, fats, and proteins are all
consumed as fuel, it is helpful to trace cellular
respiration with the sugar glucose:
C6H12O6 + 6O2  6CO2 + 6H2O + Energy (ATP + heat)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Redox Reactions: Oxidation and Reduction
• The transfer of electrons during chemical
reactions releases energy stored in organic
molecules
• This released energy is ultimately used to
synthesize ATP
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The Principle of Redox
• Chemical reactions that transfer electrons
between reactants are called oxidation-reduction
reactions, or redox reactions
• In oxidation, a substance loses electrons, or is
oxidized
• In reduction, a substance gains electrons, or is
reduced (the amount of positive charge is
reduced)
becomes oxidized
(loses electron)
Xe-
+
Y
X
+
becomes reduced
(gains electron)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Ye-
• The electron donor is called the reducing agent
• The electron receptor is called the oxidizing agent
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Some redox reactions do not transfer electrons
but change the electron sharing in covalent bonds
• An example is the reaction between methane and
oxygen
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-3
Products
Reactants
becomes oxidized
CH4
2 O2
+
CO2
C
Energy
2 H2O
+
becomes reduced
H
H
+
H
O
O
O
C
O
H
O
H
Methane
(reducing
agent)
Oxygen
(oxidizing
agent)
Carbon dioxide
Water
H
Oxidation of Organic Fuel Molecules During
Cellular Respiration
• During cellular respiration, the fuel (such as
glucose) is oxidized and oxygen is reduced:
becomes oxidized
C6H12O6 + 6O2
6CO2 + 6H2O + Energy
becomes reduced
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Stepwise Energy Harvest via NAD+ and the Electron
Transport Chain
• In cellular respiration, glucose and other organic
molecules are broken down in a series of steps
• Electrons from organic compounds are usually
first transferred to NAD+, a coenzyme
• As an electron acceptor, NAD+ functions as an
oxidizing agent during cellular respiration
• Each NADH (the reduced form of NAD+)
represents stored energy that is tapped to
synthesize ATP
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-4
2 e– + 2 H+
NAD+
2 e– + H+
H+
NADH
Dehydrogenase
+ 2[H]
(from food)
Nicotinamide
(oxidized form)
+
Nicotinamide
(reduced form)
H+
Flavin adenine dinucleotide (FAD)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• FAD reduction (FAD  FADH2)
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• NADH passes the electrons to the electron
transport chain
• Unlike an uncontrolled reaction, the electron
transport chain passes electrons in a series of
steps instead of one explosive reaction
• Oxygen pulls electrons down the chain in an
energy-yielding tumble
• The energy yielded is used to regenerate ATP
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-5
H2 + 1/2 O2
+
2H
1 /2
O2
1/2
O2
(from food via NADH)
Explosive
release of
heat and light
energy
Free energy, G
Free energy, G
2 H+ + 2 e–
Controlled
release of
energy for
synthesis of
ATP
ATP
ATP
ATP
2 e–
2
H+
H2O
Uncontrolled reaction
H2O
Cellular respiration
Schematic representation of types
of multienzyme systems carrying
out a metabolic pathway: (a)
Physically separate, soluble
enzymes with diffusing
intermediates. (b) A multienzyme
complex. Substrate enters the
complex, becomes covalently
bound and then sequentially
modified by enzymes E1 to E5
before product is released. No
intermediates are free to diffuse
away. (c) A membrane-bound
multienzyme system.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The Stages of Cellular Respiration: A Preview
• Cellular respiration has three stages:
– Glycolysis (breaks down glucose into two
molecules of pyruvate)
– The citric acid cycle (completes the breakdown of
glucose)
– Oxidative phosphorylation (accounts for most of
the ATP synthesis)
• The process that generates most of the ATP is called
oxidative phosphorylation because it is powered by
redox reactions
[Animation listed on slide following figure]
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-6_1
Glycolysis
Pyruvate
Glucose
Cytosol
Mitochondrion
ATP
Substrate-level
phosphorylation
LE 9-6_2
Glycolysis
Pyruvate
Glucose
Cytosol
Citric
acid
cycle
Mitochondrion
ATP
ATP
Substrate-level
phosphorylation
Substrate-level
phosphorylation
LE 9-6_3
Electrons carried
via NADH and
FADH2
Electrons
carried
via NADH
Glycolysis
Pyruvate
Glucose
Cytosol
Citric
acid
cycle
Oxidative
phosphorylation:
electron transport
and
chemiosmosis
Mitochondrion
ATP
ATP
ATP
Substrate-level
phosphorylation
Substrate-level
phosphorylation
Oxidative
phosphorylation
• Oxidative phosphorylation accounts for almost
90% of the ATP generated by cellular respiration
• A small amount of ATP is formed in glycolysis and
the citric acid cycle by substrate-level
phosphorylation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-7
Enzyme
Enzyme
ADP
P
Substrate
+
Product
ATP
Concept 9.2: Glycolysis harvests energy by
oxidizing glucose to pyruvate
• Glycolysis (“splitting of sugar”) breaks down
glucose into two molecules of pyruvate
• Glycolysis occurs in the cytoplasm and has two
major phases:
– Energy investment phase
– Energy payoff phase
Animation: Glycolysis
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-8
Energy investment phase
Glucose
2 ATP used
2 ADP + 2 P
Glycolysis
Citric
acid
cycle
Oxidative
phosphorylation
Energy payoff phase
ATP
ATP
ATP
4 ADP + 4 P
2 NAD+ + 4 e– + 4 H+
4 ATP formed
2 NADH + 2 H+
2 Pyruvate + 2 H2O
Net
Glucose
4 ATP formed – 2 ATP used
2 NAD+ + 4 e– + 4 H+
2 Pyruvate + 2 H2O
2 ATP
2 NADH + 2 H+
LE 9-9a_1
Glucose
ATP
Hexokinase
ADP
Glucose-6-phosphate
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
ATP
LE 9-9a_2
Glucose
ATP
Hexokinase
ADP
Glucose-6-phosphate
Phosphoglucoisomerase
Fructose-6-phosphate
ATP
Phosphofructokinase
ADP
Fructose1, 6-bisphosphate
Aldolase
Isomerase
Dihydroxyacetone
phosphate
Glyceraldehyde3-phosphate
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
ATP
LE 9-9b_1
2 NAD+
Triose phosphate
dehydrogenase
2 NADH
+ 2 H+
1, 3-Bisphosphoglycerate
2 ADP
Phosphoglycerokinase
2 ATP
3-Phosphoglycerate
Phosphoglyceromutase
2-Phosphoglycerate
LE 9-9b_2
2 NAD+
Triose phosphate
dehydrogenase
2 NADH
+ 2 H+
1, 3-Bisphosphoglycerate
2 ADP
Phosphoglycerokinase
2 ATP
3-Phosphoglycerate
Phosphoglyceromutase
2-Phosphoglycerate
2 H2O
Enolase
Phosphoenolpyruvate
2 ADP
Pyruvate kinase
2 ATP
Pyruvate
Concept 9.3: The citric acid cycle completes the
energy-yielding oxidation of organic molecules
• Before the citric acid cycle can begin, pyruvate
must be converted to acetyl CoA, which links the
cycle to glycolysis
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-10
MITOCHONDRION
CYTOSOL
NAD+
NADH
+ H+
Acetyl Co A
Pyruvate
Transport protein
CO2
Coenzyme A
The fate of pyruvate depends on the cell energy charge.
•
In cells or tissues with a high energy charge pyruvate is
directed toward gluconeogenesis,
•
but when the energy charge is low pyruvate is preferentially
oxidized to CO2 and H2O in the TCA cycle.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• The citric acid cycle, also called the Krebs cycle,
takes place within the mitochondrial matrix
• The cycle oxidizes organic fuel derived from
pyruvate, generating one ATP, 3 NADH, and 1
FADH2 per turn
Animation: Electron Transport
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-11
Pyruvate
(from glycolysis,
2 molecules per glucose)
CO2
NAD+
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
CoA
NADH
+ H+
Acetyl CoA
CoA
CoA
Citric
acid
cycle
FADH2
2 CO2
3 NAD+
3 NADH
+ 3 H+
FAD
ADP + P i
ATP
ATP
• The citric acid cycle has eight steps, each
catalyzed by a specific enzyme
• The acetyl group of acetyl CoA joins the cycle by
combining with oxaloacetate, forming citrate
• The next seven steps decompose the citrate back
to oxaloacetate, making the process a cycle
• The NADH and FADH2 produced by the cycle
relay electrons extracted from food to the electron
transport chain
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The enzymatic activities of the TCA cycle (and of oxidative phosphorylation) are
located in the mitochondrion. When transported into the mitochondrion, pyruvate
encounters two principal metabolizing enzymes: pyruvate carboxylase (a
gluconeogenic enzyme) and pyruvate dehydrogenase (PDH), the first enzyme of the
PDH complex. With a high cell-energy charge coenzyme A (CoA) is highly acylated,
principally as acetyl-CoA, and able allosterically to activate pyruvate carboxylase,
directing pyruvate toward gluconeogenesis. When the energy charge is low CoA is
not acylated, pyruvate carboxylase is inactive, and pyruvate is preferentially
metabolized via the PDH complex and the enzymes of the TCA cycle to CO2 and
H2O. Reduced NADH and FADH2 generated during the oxidative reactions can then
be used to drive ATP synthesis via oxidative phosphorylation.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-12_1
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
ATP
Acetyl CoA
H2O
Oxaloacetate
Citrate
Isocitrate
Citric
acid
cycle
LE 9-12_2
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
ATP
Acetyl CoA
H2O
Oxaloacetate
Citrate
Isocitrate
CO2
Citric
acid
cycle
NAD+
NADH
+ H+
a-Ketoglutarate
NAD+
Succinyl
CoA
NADH
+ H+
CO2
LE 9-12_3
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
ATP
Acetyl CoA
H2O
Oxaloacetate
Citrate
Isocitrate
CO2
Citric
acid
cycle
NAD+
NADH
+ H+
Fumarate
a-Ketoglutarate
FADH2
NAD+
FAD
Succinate
GTP GDP
ADP
ATP
Pi
Succinyl
CoA
NADH
+ H+
CO2
LE 9-12_4
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidation
phosphorylation
ATP
Acetyl CoA
NADH
+ H+
H2O
NAD+
Oxaloacetate
Malate
Citrate
Isocitrate
CO2
Citric
acid
cycle
H2O
NAD+
NADH
+ H+
Fumarate
a-Ketoglutarate
FADH2
NAD+
FAD
Succinate
GTP GDP
ADP
ATP
Pi
Succinyl
CoA
NADH
+ H+
CO2
Concept 9.4: During oxidative phosphorylation,
chemiosmosis couples electron transport to ATP synthesis
• Following glycolysis and the citric acid cycle,
NADH and FADH2 account for most of the energy
extracted from food
• These two electron carriers donate electrons to
the electron transport chain, which powers ATP
synthesis via oxidative phosphorylation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The Pathway of Electron Transport
• The electron transport chain is in the cristae of the
mitochondrion
• Most of the chain’s components are proteins,
which exist in multiprotein complexes
• The carriers alternate reduced and oxidized states
as they accept and donate electrons
• Electrons drop in free energy as they go down the
chain and are finally passed to O2, forming water
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-13
NADH
50
Free energy (G) relative to O2 (kcal/mol)
FADH2
40
FMN
I
Multiprotein
complexes
FAD
Fe•S II
Fe•S
Q
III
Cyt b
30
Fe•S
Cyt c1
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidative
phosphorylation:
electron transport
and chemiosmosis
IV
Cyt c
Cyt a
Cyt a3
20
10
0
2 H+ + 1/2 O2
H2O
ATP
• The electron transport chain generates no ATP
• The chain’s function is to break the large freeenergy drop from food to O2 into smaller steps that
release energy in manageable amounts
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Chemiosmosis: The Energy-Coupling Mechanism
• Electron transfer in the electron transport chain
causes proteins to pump H+ from the
mitochondrial matrix to the intermembrane space
• H+ then moves back across the membrane,
passing through channels in ATP synthase
• ATP synthase uses the exergonic flow of H+ to
drive phosphorylation of ATP
• This is an example of chemiosmosis, the use of
energy in a H+ gradient to drive cellular work
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-14
INTERMEMBRANE SPACE
H+
H+
H+
H+
H+
H+
A rotor within the
membrane spins
as shown when
H+ flows past
it down the H+
gradient.
H+
A stator anchored
in the membrane
holds the knob
stationary.
A rod (or “stalk”)
extending into
the knob also
spins, activating
catalytic sites in
the knob.
H+
ADP
+
P
ATP
i
MITOCHONDRAL MATRIX
Three catalytic
sites in the
stationary knob
join inorganic
phosphate to
ADP to make
ATP.
• The energy stored in a H+ gradient across a
membrane couples the redox reactions of the
electron transport chain to ATP synthesis
• The H+ gradient is referred to as a proton-motive
force, emphasizing its capacity to do work
Animation: Fermentation Overview
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-15
Inner
mitochondrial
membrane
Glycolysis
Citric
acid
cycle
ATP
ATP
Oxidative
phosphorylation:
electron transport
and chemiosmosis
ATP
H+
H+
H+
H+
Intermembrane
space
Cyt c
Protein complex
of electron
carriers
Q
IV
III
I
ATP
synthase
II
Inner
mitochondrial
membrane
FADH2
NADH + H+
2H+ + 1/2 O2
H2O
FAD
NAD+
Mitochondrial
matrix
ATP
ADP + P i
(carrying electrons
from food)
H+
Electron transport chain
Electron transport and pumping of protons (H+),
Which create an H+ gradient across the membrane
Oxidative phosphorylation
Chemiosmosis
ATP synthesis powered by the flow
of H+ back across the membrane
An Accounting of ATP Production by Cellular
Respiration
• During cellular respiration, most energy flows in
this sequence:
glucose NADH electron transport chain
proton-motive force ATP
• About 40% of the energy in a glucose molecule is
transferred to ATP during cellular respiration,
making about 38 ATP
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-16
Electron shuttles
span membrane
CYTOSOL
2 NADH
Glycolysis
Glucose
2
Pyruvate
MITOCHONDRION
2 NADH
or
2 FADH2
2 NADH
2
Acetyl
CoA
6 NADH
Citric
acid
cycle
+ 2 ATP
+ 2 ATP
by substrate-level
phosphorylation
by substrate-level
phosphorylation
Maximum per glucose:
About
36 or 38 ATP
2 FADH2
Oxidative
phosphorylation:
electron transport
and
chemiosmosis
+ about 32 or 34 ATP
by oxidation phosphorylation, depending
on which shuttle transports electrons
form NADH in cytosol
Concept 9.5: Fermentation enables some cells to
produce ATP without the use of oxygen
• Cellular respiration requires O2 to produce ATP
• Glycolysis can produce ATP with or without O2 (in
aerobic or anaerobic conditions)
• In the absence of O2, glycolysis couples with
fermentation to produce ATP
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Types of Fermentation
• Fermentation consists of glycolysis plus reactions
that regenerate NAD+, which can be reused by
glycolysis
• Two common types are alcohol fermentation and
lactic acid fermentation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• In alcohol fermentation, pyruvate is converted to
ethanol in two steps, with the first releasing CO2
• Alcohol fermentation by yeast is used in brewing,
winemaking, and baking
Play
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-17a
2 ADP + 2 P i
Glucose
2 ATP
Glycolysis
2 Pyruvate
2 NAD+
2 Ethanol
Alcohol fermentation
2 NADH
+ 2 H+
2 CO2
2 Acetaldehyde
• In lactic acid fermentation, pyruvate is reduced to
NADH, forming lactate as an end product, with no
release of CO2
• Lactic acid fermentation by some fungi and
bacteria is used to make cheese and yogurt
• Human muscle cells use lactic acid fermentation to
generate ATP when O2 is scarce
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-17b
2 ADP + 2 P i
Glucose
2 ATP
Glycolysis
2 NAD+
2 NADH
+ 2 H+
2 CO2
2 Pyruvate
2 Lactate
Lactic acid fermentation
Lactic acid fermentation
In vertebrates, a second pathway anaerobic pathway occurs when oxygen
levels drop. Pyruvate is converted to lactic acid where NADH serves as the
reducing agent.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Fermentation and Cellular Respiration Compared
• Both processes use glycolysis to oxidize glucose
and other organic fuels to pyruvate
• The processes have different final electron
acceptors: an organic molecule (such as pyruvate)
in fermentation and O2 in cellular respiration
• Cellular respiration produces much more ATP
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
• Yeast and many bacteria are facultative
anaerobes, meaning that they can survive using
either fermentation or cellular respiration
• In a facultative anaerobe, pyruvate is a fork in the
metabolic road that leads to two alternative
catabolic routes
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-18
Glucose
CYTOSOL
Pyruvate
No O2 present
Fermentation
O2 present
Cellular respiration
MITOCHONDRION
Ethanol
or
lactate
Acetyl CoA
Citric
acid
cycle
The Evolutionary Significance of Glycolysis
• Glycolysis occurs in nearly all organisms
• Glycolysis probably evolved in ancient prokaryotes
before there was oxygen in the atmosphere
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Concept 9.6: Glycolysis and the citric acid cycle
connect to many other metabolic pathways
• Gycolysis and the citric acid cycle are major
intersections to various catabolic and anabolic
pathways
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The Versatility of Catabolism
• Catabolic pathways funnel electrons from many
kinds of organic molecules into cellular respiration
• Glycolysis accepts a wide range of carbohydrates
• Proteins must be digested to amino acids; amino
groups can feed glycolysis or the citric acid cycle
• Fats are digested to glycerol (used in glycolysis)
and fatty acids (used in generating acetyl CoA)
• An oxidized gram of fat produces more than twice
as much ATP as an oxidized gram of carbohydrate
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-19
Proteins
Carbohydrates
Amino
acids
Sugars
Glycerol Fatty
acids
Glycolysis
Glucose
Glyceraldehyde-3- P
NH3
Fats
Pyruvate
Acetyl CoA
Citric
acid
cycle
Oxidative
phosphorylation
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Biosynthesis (Anabolic Pathways)
• The body uses small molecules to build other
substances
• These small molecules may come directly from
food, from glycolysis, or from the citric acid cycle
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Regulation of Cellular Respiration via Feedback
Mechanisms
• Feedback inhibition is the most common
mechanism for control
• If ATP concentration begins to drop, respiration
speeds up; when there is plenty of ATP, respiration
slows down
• Control of catabolism is based mainly on
regulating the activity of enzymes at strategic
points in the catabolic pathway
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
LE 9-20
Glucose
AMP
Glycolysis
Fructose-6-phosphate
–
Stimulates
+
Phosphofructokinase
–
Fructose-1,6-bisphosphate
Inhibits
Inhibits
Pyruvate
ATP
Citrate
Acetyl CoA
Citric
acid
cycle
Oxidative
phosphorylation
Parallel pathways of catabolism and anabolism must differ in at least one metabolic
step in order that they can be regulated independently. Shown here are two possible
arrangements of opposing catabolic and anabolic sequences between A and P. (a) The
parallel sequences proceed via independent routes. (b) Only one reaction has two
different enzymes, a catabolic one (E3) and its anabolic counterpart (E6). These
provide sites for regulation.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Regulation of glycolysis and gluconeogenesis by fructose 2,6-bisphosphate (F2,6BP). The major sites for regulation
of glycolysis and gluconeogenesis are the phosphofructokinase-1 (PFK-1) and fructose-1,6-bisphosphatase (F-1,6BPase) catalyzed reactions. PFK-2 is the kinase activity and F-2,6-BPase is the phosphatase activity of the bifunctional regulatory enzyme, phosphofructokinase-2/fructose-2,6-bisphosphatase. PKA is cAMP-dependent protein
kinase which phosphorylates PFK-2/F-2,6-BPase turning on the phosphatase activity. (+ve) and (-ve) refer to
positive and negative activities, respectively.
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Regulation of the Krebs cycle
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings