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STANDARD COURSE SYLLABUS
for academic year 2012/2013
Name of subject:
Description of subject matter – Instructional program
Biochemistry
Module code according to standards from A
to G
B.W1, B.W2, B.W11-21, B.W24, B.W26, B.W34.
B.U6-7, B.U9-11, B.U14
Director of unit
conduction the course:
Faculty:
Course of study:
Level of studies
prof. dr hab. Andrzej
Gamian
Medical
Medical
Unitary MMed
Form of studies
Year:
Type of subject
Language of
instruction:
full-time X extramural
II
obligatory X elective 
English
Name of unit conducting course
Department of Medical Biochemistry
Semester:
Winter semester (hrs.)
L
C
S
30
40
10
III i IV
Summer semester (hrs.)
L
C
S
20
40
10
Total: 150 hrs
Educational goals (goals for lessons set by instructor, related to the results of education, max. 6 items)
C1. Acquisition of the knowledge on the metabolism of the main groups of chemical compounds in
physiological conditions.
C2. Understanding the processes leading to the diversion of normal into pathological biochemical
pathways, and molecular basis of chosen disorders development
C3. Acquaintance with the basic scientific techniques applied in biochemistry.
C4. Familarity with the analysis and interpretation of the results obtained in biochemical experiments
(calculations, graphs analysis etc.).
C5. The formation of an essential biochemical foundation for further insight into molecular processes in
pathological conditions and the possibilities of their regulation, in the subsequent steps of medical
education.
Matrix of educational results for subjects in reference to methods for verifying intended
educational results and manner of conducting lessons.
Number Description of educational result (in conformance with detailed
Methods for
educational results defined in standards)
of
verifying
educatio
achievement
nal
of intended
result
educational
results *
Describes biochemical regulation of water-electrolite
test,
B.W1.
metabolism in biological systems;
presentation,
Manner of
lessons:
** provide
symbol
L, S, C
B.W2.
B.W11.
B.W12.
B.W13.
B.W14.
B.W15.
B.W16.
B.W17.
B.W18.
B.W19.
B.W20.
B.W21.
B.W24.
B.W26.
B.W34.
B.U6.
B.U7.
B.U9.
Describes acid-base balance and the mechanism of buffering in
physiological fluids, and their significance in the organism
homeostasis;
Describes the structure of lipids and polysaccharides, and their
functions in cellular and extracellular structures;
Knows primary, secondary, tertiary and quaternary structures of
proteins; knows posttranslational and functional protein
modifications and their significance;
Knows the functions of nucleotides in the cell, primary and
secondary structures of DNA and RNA as well as chromatin
structure;
Knows the functions of genome, transcriptome and proteome of
the human, and the basic methods applied in their analysis;
describes the processes of replication, repairing and
recombination of DNA, transcription and translation, as well as
degradation of DNA, RNA and proteins; knows the idea of the
regulation of gene expression;
Describes the basic catabolic and anabolic pathways, the modes
of their regulation, and the impact of genetic and environmental
factors;
Knows metabolic profiles of the basic organs and systems;
Knows the concepts of oxidative potential of the organism and
oxidative stress;
Knows the enzymes involved in digestion, the mechanism of
hydrochloric acid generation in the stomach, the role of the bile,
the process of the absorption of digestion products, and its
disturbances;
Knows the consequences of an improper diet including a longtime starvation as well as the intake of an excess-calories meals
and unbalanced diet;
Knows the consequences of vitamins and minerals deficiency or
excess in the organism;
Knows the biochemical pathways of the communication between
cells, between the cell and extracellular matrix, as well as signal
transduction pathways in the cell, and the examples of
disturbances in these processes resulting in the onset of cancer
and other diseases;
Knows the biochemical background of nerve signal
stransmission in the nervous system, and higher nervous
functions, as well as the biochemistry of the skeletal and smooth
muscles, and biochemical functions of the blood;
Knows biochemical mechanisms of hormones actions, and the
consequences of disturbances in hormonal regulation;
Knows the principles of the experimental biochemical
investigations as well as in vitro studies serving the development
of medicine;
Can foresee the direction of biochemical processes on the basis
of the energetic status of the cell;
Describes biochemical changes in the organism functioning in
the situation of homeostasis disturbance, especially defines its
biochemical response to physical exercise, exposition to high or
low temperature, the loss of blood or water;
Can use basic laboratory techniques such as quality analysis,
titration, colorimetry, pehametry, chromatography,
oral response,
report,
colloquium,
written
examination
test,
S, C
presentation,
oral response,
report,
colloquium,
written
examination
electrophoresis of proteins and nucleic acids applied in
biochemistry;
Handles simple measurement utilities applied in biochemistry,
B.U10.
and evaluates the precision of the performed measurements;
Takes advantage of biochemical data bases including the
B.U11.
Internet ones, and is able to search for the required information
with the aid of the available tools;
Can design and perform a simple biochemical scientific
B.U14.
investigation, interpret the obtained results, and draw
conclusions.
*e.g.. test, presentation, oral response, essay, report, colloquium, oral examination, written examination;
** L- lecture; S- seminar; C- class; EL- e-learning;
Student work input (balance of ECTS points)
Lessons on-site (hrs.) 150 hrs
Own work (hrs.) 67 hrs
Summary of student workload
217 hrs
ECTS points for subject
14
Remarks
Content of lessons: (please provide the subject of individual lessons, keeping in mind the need to contribute to the intended
educational results)
Lectures:
1. The structure of peptides and proteins. Peptide hormones.
2. Structure and functions of fibrous and globular proteins.
3. Enzymes – properties and kinetics.
4. Mechanisms of action and regulation of enzyme activity.
5. Transport across membranes.
6. Catabolic processes. Tricarboxylic acid cycle.
7. Respiratory chain and oxidative phosphorylation. Oxidative stress and antioxidant potential of the
organism.
Structure and function of carbohydrates.
Glucose metabolism.
Glycogen metabolism, fructose and galactose metabolism.
Regulation of carbohydrate metabolism. Heteroglycan metabolism.
Functions of lipids. Transport of dietary and endogenous lipids.
Oxidation and biosynthesis of fatty acids; regulation.
8.
9.
10.
11.
12.
13.
14. Triacylglycerols metabolism. Connections between lipid and carbohydrate metabolism.
15. Cholesterol metabolism and its regulation, disorders.
16. Steroid hormones.
17. Nitrogen metabolism – amino acid metabolism.
18. Porphyrins and bile pigments.
19. Nucleotide metabolism.
20. Hormonal regulation of metabolic processes.
21. Iron metabolism and calcium-phosphate metabolism.
22. Metabolism of xenobiotics.
23. Replication and repair of DNA, RNA metabolism.
24. Protein synthesis, genetic code, regulation of gene expression.
25. Biochemical basis of selected diseases.
Laboratories:
1. Protein determination using Biuret method
2. Determination of inorganic phosphate by Fiske-Subbarow method.
3. Studies on kinetics of phosphatase reaction.
4. Examination of enzymatic reaction catalyzed by succinate dehydrogenase.
5. Quantitative determination of vitamin C.
6. Determination of catalase activity.
Examination of enzymatic reaction catalyzed by horseradish peroxidase.
Quantitative determination of reducing sugars.
Determination of glucose using glucose oxidase.
Effect of pH on saccharase activity.
Substrate specificity and inhibitors of saccharase.
Glycogen degradation by muscle pulp's enzymes.
Repetition of laboratories not credited in the winter semester.
Hydrolysis of lipids. Determination of lipase activity.
Determination of -lipoproteins concentration (LDL).
Determination of triglycerides.
Cholesterol determination (HDL).
Examination of transaminase reaction.
Quantitative determination of creatinine.
Quantitative determination of uric acid.
Quantitative determination of urea in urine.
Determination of hemoglobin and its derivatives.
Determination of GGT activity in serum.
Quantitative determination of total bilirubin.
Quantitative determination of calcium.
Repetition of laboratories not credited in the summer semester.
Seminars:
1. Peptides and proteins – methods of separation and isolation.
2. Kinetics of enzymatic reactions.
3. Regulation of metabolism by control of enzymatic activity.
4. Oxidoreductases and their cofactors.
5. Biological membranes functions, transport cross membranes.
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
6. Regulation of tricarboxylic acid cycle and the connections with other metabolic pathways.
7. Pentose phosphate pathway.
8. Fructose and galactose metabolism.
9. Regulation of carbohydrate metabolism.
10. Credits.
11. Digestion, absorption and transport of lipids. Adipose tissue.
12. Chemical structure and the role of steroid compounds.
13. Bile acids metabolism.
14. General metabolism of nucleotides. Biologically active amines.
15. Urea cycle.
16. Endogenous regulators of metabolic processes.
17. Liver biochemistry.
18. Connective tissue biochemistry.
19. Muscle biochemistry.
20. Credits.
Primary and secondary literature
Required literature :
1. Richard A. Harvey et al. “Lippincot’s Illustrated Reviews: Biochemistry”
2. Michael Lieberman, Allan D. Marks “Mark’s Basic Medical Biochemistry: A Clinical Approach”,
Third Edition
3. Robert K. Murray et al. "Harper's Biochemistry"
Additional, suggested reading:
1. Thomas M.Devlin „Biochemistry with Clinical Correlations”, Willey-Liss, New York, 2002.
2. Gerard Meisenberg, Wiliam H. Simmons “Principles of Medical Biochemistry” Mosby Elsevier, 2nd
Edition, 2006
3. L. Baynes., M. Dominiczak, „Medical Biochemistry”, Mosby Elsevier, Third Edition 2009.
4. J. Koolman and K.-H. Rohm "Color Atlas of Biochemistry" – Thieme
5. Lubert Stryer "Biochemistry"
6. R. H. Garrett and C. M. Grisham "Biochemistry" - Saunders College Publishing, Harcourt Brace
College Publishers.
Requirements concerning instructional aids (e.g. laboratory, multimedia projector, other …)
1. Laboratories, seminar rooms, lecture halls.
2. Laboratory utilities; water baths, centrifuges, incubators, spectrophotometers, glassware, pippets.
3. Multimedia projectors, whiteboards.
Conditions for successful completion of course:
1. Obtaining credits for all laboratories, and passing all midterm tests.
2. Passing the final exam (60% of correct answers are required at all levels of examination).
Name and address of unit conducting course, contact information (tel./email):
Department of Medical Biochemistry, phone: 784-13-82, e-mail:
[email protected]
Person responsible for the course for a given year
Dr Małgorzata Matusiewicz
Signature of head of unit conducting the course
Signature of dean
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Date of syllabus drafting: ……………………………………………