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-Abstract form (English version)Title.......................................... (Cordia New, 16pt, bold, Center)
Student name1, Student name1, Supervisor name2* (Cordia New, 14pt, bold, Center)
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Department of ................., Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
Department of ................., Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
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Address of organization / Institute………………………………………………………….
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*Corresponding author; *E-mail: Corresponding author (Cordia New, 12pt, Regular, Center)
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Medical Science Academic Annual Meeting 2013, 3-5th March 2013, Faculty of Medical Science, Naresuan U., Phitsanulok
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-ตัวอย่ างบทคัดย่ อภาษาอังกฤษAssociation Study of Regulators of G- protein Signaling 2 (RGS2) gene polymorphism with Obesity in
the Malaysian Malays
Narong Jaturas1, Yamunah Devi Apalasamy2*, Moy Foong Ming3, Sanjay Rampal3, Awang Bulgiba3,
Zahurin Mohamed2
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Faculty of Medical Science, Naresuan University, Phitsanulok, Thailand 65000
Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia 50603
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Department of Social and Preventive Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur,
Malaysia 50603
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*Corresponding author; *E-mail: [email protected]
Abstract
Obesity is increasingly a health problem and risk factor of morbidity in the Malaysian Malays population. A
large number of genes are involved in adipose tissue deposition, including genes coding for G- protein called RGS
(Regulators of G protein Signaling) terminates G-protein signaling by accelerating the rate of guanosine
triphosphate (GTP) hydrolysis by Gα subunits. In addition, RGS2 is the principle RGS protein, was strongly
expressed at the beginning of adipocyte differentiation. Previous studies have shown that common polymorphisms
of RGS2 gene are associated with increased BMI and greater risk of developing overweight or obesity. In this study,
we aimed to investigate whether the rs4606 polymorphism of the RGS2 gene is associated with obesity in the
Malaysian Malays population. RGS2 C1114G SNP was genotyped in 562 ( Non- obese, n = 419, Obese, n = 143)
Malaysian Malays subjects (age, 47.15±8.91 years) from DNA extracted from buccal swabs. The SNP was analyzed
using real- time polymerase chain reaction. The genotype and allele frequencies were calculated using Chi-square
tests. The RGS2 rs4606 SNP was in Hardy-Weinberg equilibrium for non- obese and obese groups. There were no
significant differences between obese and non-obese groups in either genotype (P=0.383) or allele frequencies
(P=0.182) in rs4606. Binary regression was used to analyze the association between RGS2 rs4606 SNP with obesity.
There was no significant association between RGS2 rs4606 SNP with obesity in the Malaysian Malays (OR, 1.22;
95% confidence interval (CI), 0.94- 1.59; P= 0.135).
Key words: Regulators of G- protein Signaling 2 gene (RGS2), rs4606, Single Nucleotide Polymorphisms, Obesity
Medical Science Academic Annual Meeting 2013, 3-5th March 2013, Faculty of Medical Science, Naresuan U., Phitsanulok
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Association of the glutamate transporter EAAT2 and the glutamate receptor GRM3 gene
polymorphism with methamphetamine dependence
Walailuk Kerdsan1, Samur Thanoi1, Paritat Watiktinkorn2, Gavin P. Reynolds3, Sutisa Nudmamud-Thanoi1*
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Department of Anatomy, Faculty of Medical Science, Phitsanulok, Thailand 65000
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Synphaet hospital, Bangkok, Thailand 10230
3
Biomedical Research Centre, Sheffield Hallam University, Sheffield S10 4LN U.K.
*Corresponding author; *E-mail: [email protected]
Abstract
Research strongly implicates glutamatergic system in etiology of drug dependence. Changes in glutamate
transporter EAAT2 protein expression have been reported after exposure to methamphetamine and cocaine. EAAT2
gene variant have been associated with schizophrenia, some positive symptoms are similar to those occurring in
methamphetamine dependence. Agonist to glutamate receptor GRM3 has been reported to inhibit amphetamineinduced behavioral and cognitive abnormalities. Moreover, polymorphism of the GRM3 gene has been associated
with a low of EAAT2 mRNA level and an impairment of cognitive function in schizophrenia. Therefore, the aim of this
study was to investigate the association of EAAT2 and GRM3 gene polymorphisms with methamphetamine
dependence. One hundred methamphetamine dependence, meeting DSM-IV criteria, and 102 ethnically-matched
healthy subjects were genotyped for EAAT2 (rs1885343) gene and GRM3 (rs6465084) gene polymorphisms from
blood using PCR-RFLP and real-time PCR methods, respectively. The genotype and allele frequencies were
analyzed using Chi-square and Fisher’s exact tests. The polymorphisms for both control and methamphetamine
groups were in Hardy-Weinberg equilibrium. There were no significant difference between groups in either genotype
(P=0.117) or allele frequencies (P=0.228) in rs1885343. Additionally, a significant excess of G/G genotype versus A
allele carriers was also observed in rs1885343 in methamphetamine group relative to control group (P=0.010). For
the rs6465084, statistical differences between the groups were not detected in either genotype (P=0.951) or allele
frequencies (P=0.777). The results revealed an association between EAAT2 gene polymorphism and
methamphetamine dependence. This finding is consistent with a study found an association of the EAAT2
polymorphism with schizophrenia. Moreover, genetic variation of the EAAT2 gene has been contributed to alcoholic
behavior. Our findings suggest that polymorphism of the EAAT2 gene, but not the GRM3 gene may confer
vulnerability to a risk factor for methamphetamine dependence.
Key words: Methamphetamine, Glutamate, EAAT2 gene, GRM3 gene, Polymorphism
Medical Science Academic Annual Meeting 2013, 3-5th March 2013, Faculty of Medical Science, Naresuan U., Phitsanulok
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