Download Custom Protein Order Information

Custom Protein Order Information
Introduction：
Please fill in the following information in detail and send the complete form to [email protected].
Our technical staff will make professional assessment according to the information you provide and send
the assessment result to your mailbox at the first time.
Thanks for your trust and support for Elabscience!
Customer Information (This information is required, please fill in it in detail.)
Name
Telephone
Email
Institution name
Delivery address
Postcode
Notes
Protein information
Protein name
Species /
Entrez Gene
SwissProt
Gene sequence
Gene synthesis (with codon optimization)
Gene synthesis (not required codon optimization)
Customer provide the DNA template:
Vector name:
Gene source
(Please provide the vector map in
the form of mail accessory, such as NTI file.)
Customer provide the expression recombinant plasmid:
Vector sequence: Please provide the vector map in the form of
mail accessory, such as NTI file, and mark the cloning site in
the vector map).
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Tel: 1-240-252-7368
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Other (※please provide the detailed information):
Please choose the expression system of the target protein:
E coli.
Yeast
Baculovirus/Insect cells
Mammalian cells
If you choose the mammalian cells expression system, please
choose the corresponding host cell:
HEK293
CHO
Others:
No specific requirements.
Note: If you choose “No specific requirements”, we will use the
HEK293 as host cell by default.
Have you ever expressed the target protein before?
Yes
Expression system
No
If you have ever expressed this protein, please tell us the specific
expression system:
E coli.
Yeast
Baculovirus / Insect cells
Mammalian cells
Other system:
Protein expression amount:
Solubility:
mg;
;
Details of the experimental procedures and results:
;
Other information:
Note: The above information will be of great help to our
assessment, please complete it in detail.
Which secreted signal peptide do you prefer to choose for the
expression of your target protein?
Secreted signal
Signal peptide sequence of the target protein
peptide sequence:
No specific requirements
(Specific for insect
Others (please provide detailed information):
.
cells expression
system and
Please choose the certain expression pattern:
mammalian cells
Secretory expression (Elabscience will purify the target protein
expression system.)
from the culture medium)
If only a small amount of the target protein can be obtained from
the culture medium, then we will try to purify the protein from the
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Tel: 1-240-252-7368
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cell lysate (needs extra payment).
Make expression evaluation via two kinds of plans:
One is to use the signal peptide to perform the secretory
expression, while the other one is to carry out the intercellular
expression without adding the signal peptide.
Membrane proteins
Secretory proteins
Protease
Other enzymes
Characteristics of the
Intercellular proteins
target protein
IgG antibody
Toxic proteins
Others (please provide the detailed information):
.
High throughput screening (HTS)
Activity detection
The subsequent
Nuclear magnetic resonance (NMR)
application purpose
X-ray crystallization
of the target protein
Antigen
(please choose the
In vivo activity study
application area)
Protein-protein interaction
Others (please provide detailed information):
.
Requirements of protein
Purification
_______%
Endotoxin
requirement
Concentration
Amplification
Protein amount: _____ mg.
expression service
Or the demanded volume: _______ liter(s).
Do you need Elabscience to customize the program of purifying
the target protein?
Yes
No, I would like to use my own purification method (※ please
provide the detailed information).
Purification method
Do you need Elabscience to perform the expression and
purification service according to your standard operation process?
Yes (※ please provide the detailed information) ;
No
Note: The experiment method you provide will be of great help for
improving the success rate of expression and shorten the service
cycle.
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Tel: 1-240-252-7368
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3
Do you need Elabscience to add tag (such as 6×His, GST) to
make convenience for the subsequent protein purification?
Yes
No, I would like to choose my own tag (※ please provide the
detailed information) .
Do you need Elabscience to add special tag to make convenience
for increasing the solubility and expression level of the target
protein?
Yes
Tag information
No, I would like to choose my own tag (※ please provide the
detailed information) .
Do you need Elabscience adding the tag to N-terminal of the
target protein?
Yes
No, I need to add tag to other sites (※ please provide the
detailed information) .
Note: If you need to add tag to other sites, please provide the
detail sequence after the tag, and make annotation and illustration
for the corresponding site.
Do you need to add protease cleavage site between the target
protein and tag?
Yes
No
If you choose “Yes”, please choose the certain protease cleavage
site:
Entrust Elabscience to choose
Tag removal service
Enterokinase
Thrombin
Do you need Elabscience to offer the tag removal service after the
protein purification?
Yes
No
Small protein tags (such as His) can be retained after
purification
Note: The removal efficiency of tags depends on the property of
the protein itself.
Do you need Elabscience trying to perform the renaturation
Renaturation service
service if the protein expressed is not soluble?
Yes
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No
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Identification method
SDS-PAGE
WB
N-terminal sequence
ELISA
Others
Special requirements
(buffer requirements
and operation
requirements, etc.)
Additional requirement
Project information
This inquiry will be used to apply for the budget
Budget information
This project has passed the budget application
Right now
Within 1 month
Within 3 months
Within 6 months
Launch time
Date:
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