Download GenScript - Protein Services

Protein Expression and Purification Service Quotation
Request Form
Customer Information
If you have registered an account with GenScript, you can just identify yourself by giving us your name and email
address or Account No.
Name:
Account No.:
Phone:
Organization:
Shipping address:
(Necessary to determine
shipping cost)
Email address:
Protein Expression
Protein name:
Target protein:
Species:
MW:
Protein sequence: (crucial for evaluation)
or accession number:
Note: Please enter the protein sequence if mutants are included in your protein sequence.
Gene synthesis with codon optimization
(Please enter your gene sequence:
)
Gene synthesis without codon optimization
(Please enter your gene sequence:
Starting material:
)
Your supplied template DNA
(Please enter your gene sequence:
, vector name:
and attach vector map
(e.g. NTI file)
Your supplied expression ready construct
(Please give a brief description of your expression construct:
)
Others (Please specify):
Please indicate the preferred expression host system:
Bacteria
Yeast
Baculovirus/insect cell
Mammalian cell
If mammalian cell expression system is preferred, please select your preferred host cell
line:
HEK293
Expression system:
CHO
Others (Please specify):
No preference
Notes: If you DO NOT have preference, HEK293 or its derivatives will be used as default
cell line in our platform.
Has the gene been expressed in any host before?
Yes
No
If YES, please indicate the host:
Bacteria
Yeast
Baculovirus/insect cell
Mammalian cell
Others (Please specify):
Yield:
Solubility:
Protocol:
Other information:
Notes: The above information is crucial for us to evaluate your project.
Do you have any preferred secretion signal sequence for expressing your protein of
interest?
Its own signal peptide
No preference
Others (Please specify):
Secretion signal
sequence: (For insect
cell and mammalian cell
expression systems)
Please specify your preferred expression format:
Secretory expression only (GenScript will only purify the protein from the medium)
Protein purification from the cell lysate if very little protein can be obtained from the
medium (Extra fee is charged)
Expression evaluations on both secretory expression with the signal peptide and
intracellular expression without the signal peptide (Recommended)
Expression scale:
Protein properties:
Amount of protein:
mg or Volume of culture required:
Membrane-bound
Secreted
A protease
Cytoplasmic protein
IgG antibody
Toxic to E.coli
liter(s)
Other enzyme
Other features that may cause difficulty in either expression or purification (Please
specify):
Application(s) of your
protein: (Please select all
that apply)
High-throughput screening (HTS)
Activity assays
NMR
X-ray crystallography
Antigen
in vivo studies
Protein-protein interactions
Others (Please specify):
Comments:
Protein Purification
Product requirements:
Purity:
%
Endotoxin Limit:
Concentration:
Do you want GenScript to recommend a purification system for purifying your protein of
interest?
Yes
Purification method:
No, I want to choose my own purification system (Please specify):
Do you need GenScript to follow any specific expression and/or purification protocol?
Yes (Please attach your protocol:
)
No
Note: Your protocol will be very helpful to increase success rate and shorten the delivery
time.
Do you want GenScript to select 1 or 2 tags (6xHis, GST, or MBP) to facilitate purification?
Yes
Tag(s) selection:
No, I want to choose my own tag(s) (Please specify):
Do you want GenScript to select additional 1 or 2 tags (Trx, TF, Dsb, or Nus) to increase
solubility and/or expression level? (Recommended—can greatly improve yield; the tag(s)
will be added to N-terminus)
Yes
No, I want to choose my own tag(s) (Please specify):
Do you want GenScript to add the tag(s) at the N-terminus?
Yes
No, I have designated place(s) to add the tag(s) (Please specify):
Note: If internal tag(s) is/are involved, please designate the position number(s) that
corresponds to the sequence of which the accession number you have indicated above.
Do you want GenScript to add a protease cleavage site between the tags and the target
protein?
Yes
No
If YES, please specify the protease you want to add:
GenScript’s choice
Tag(s) removal:
Enterokinase
PreScission
rTev
Thrombin
Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we
DO NOT recommend the introduction of protease cleavage site between the target protein
and the C-terminal tag.
Do you want GenScript to remove tag(s) in the purification step?
Yes
No
Small tag (e.g. His) can be retained after purification
Note: The recovery rate varies with the characteristics of the protein.
If the expressed protein is insoluble, do you want GenScript to attempt the refolding?
Refolding:
Yes
No
Note: GenScript will NOT charge extra fee for conventional refolding.
Protein
characterization:
SDS-PAGE
N-terminal sequence
IEF
ELISA
MALDI-TOF
HPLC
Endotoxin
Others (Please specify):
Special requirements:
(information on buffers or
handling, etc.)
Comments:
Project Information
Nature of inquiry:
Order initiation:
Pricing estimation
Quote for ordering
For grant application purpose
Immediately
Within one month
Within three months
Within six months