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NMEICT PROJECT Theory module: 07 (MCQ) INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY 1. Who coins the term Enzyme? A. Louis Pasteur B. Wilhelm Kühne C. James Sumner D. Entic Zymiq 2. Which of the following statement about enzyme is incorrect? A. All enzymes are protein excepet catalytic RNA B. Enzymes are specific in action C. All enzymes requires the cofactor D. None of these 3. Turnover number is A. The total number of products produced by a given enzyme in a given time B. Amount of product formed in reaction C. Amount of reactant react D. None of the above 4. The first enzyme to be purified and crystallized was A. Urease B. Diastase C. Insulin D. Zymase 5. Most of the members of vitamin B complex act as A. Cofactor B. Coenzyme C. Prosthetic group D. Apoenzyme 6. Ribozyme is A. RNA with phosphate B. RNA with sugar C. RNA with enzyme activity D. RNA with extra phosphate 7. The non protein part of an enzyme is known as A. Holoenzyme B. Apoenzyme C. Vitamin D. Prosthetic group 8. The protein part of an enzyme is known as A. Apoenzyme B. Holoenzyme C. Prosthetic group D. Zymogen DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 1 NMEICT PROJECT 9. Undefined A. Apoenzyme = Holoenzyme + Coenzyme B. Holoenzyme = Apoenzyme + Coenzyme C. Coenzyme = Apoenzyme + Holoenzyme D. Holoenzyme = Coenzyme + Apoenzyme 10. Which of the following part of enzyme is inactive and low molecular weight? A. Apoenzyme B. Coenzyme C. Holoenzyme D. None of the above DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 2 NMEICT PROJECT Theory module: 07 (Assignment) INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY Answer specifically 1. Define enzyme 2. Who coined the word enzyme 3. What is the active site of enzyme 4. What are the components of enzyme 5. What are the properties of enzyme 6. What are the classes of enzyme 7. How enzyme works 8. How enzyme activity can be measured 9. What are the units used to express enzyme activity 10. What is specific activity of enzyme DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 3 NMEICT PROJECT Theory module: 07 (References) INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY Lehninger Principles of Biochemistry By David. L. Nelson and Michael. M. Cox Outlines of Biochemistry by E.E Conn and P.K.Stump Biochemistry by Stryer.L Harper’s review of Biochemistry by by Martin. D. W, Mayes. P. A and Rodwell. V. M Practical of Biochemistry by Wilson & Walker Microbiology by Precott, LM, Harley, JP, Klein, DA. The McGraw-Hill Company, Sixth edition. DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 4 NMEICT PROJECT Theory module: 05 (Edutainment) INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 5 NMEICT PROJECT Across 38. These enzymes carry out the hydrolytic cleavage of C-O, C-N, C-C and some other.: 51. biocatalyst which alters the rate of reaction without undergoing any permanent change in themselves 71. A non protein organic substance loosely attached to the enzyme and can be separated by dialysis and is essential for enzyme action. 79. _________activity, It is defined as the amount of substrate the enzyme converts (reactions catalyzed), per mg protein in the enzyme preparation, per unit of time 86. This class of enzyme will carry out the joining of two molecules coupled with the hydrolysis of a ATP or a similar triphosphate 56. RNAase degrade RNA molecules of every kind irrespective of their type. Tell the type of specificity. 1. The proteins which yields aminoacids or their derivatives on hydrolysis are called __________ proteins. 23. 3D structure formed by amino acids which are being positioned far apart in the polypeptide chain Down 35. These are multiple forms of the given enzyme that occurs within the same animal species. 43. Suffix __________ should be used only for single enzymes 51. All __________ are made up of protein but all proteins are not made up of _______________. 1. Absolute group _________. Ex.: Trypsin and pepsin are proteolytic enzymes, but they cleave peptide bonds at specific amino acids. Tell the type of specificity. 50. Who coins the term enzyme? 9. These class of enzymes will transfer a group, like a methyl group or a glycosyl group, from one compound (donor) to another compound (acceptor) 14. James Sumner in 1926 demonstrate that the enzyme are protein in nature by isolating ___________ in a pure form and crystallized it DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 6