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NMEICT PROJECT
Theory module: 07 (MCQ)
INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY
1. Who coins the term Enzyme?
A. Louis Pasteur
B. Wilhelm Kühne
C. James Sumner
D. Entic Zymiq
2. Which of the following statement about enzyme is incorrect?
A. All enzymes are protein excepet catalytic RNA
B. Enzymes are specific in action
C. All enzymes requires the cofactor
D. None of these
3. Turnover number is
A. The total number of products produced by a given enzyme in a given time
B. Amount of product formed in reaction
C. Amount of reactant react
D. None of the above
4. The first enzyme to be purified and crystallized was
A. Urease
B. Diastase
C. Insulin
D. Zymase
5. Most of the members of vitamin B complex act as
A. Cofactor
B. Coenzyme
C. Prosthetic group
D. Apoenzyme
6. Ribozyme is
A. RNA with phosphate
B. RNA with sugar
C. RNA with enzyme activity
D. RNA with extra phosphate
7. The non protein part of an enzyme is known as
A. Holoenzyme
B. Apoenzyme
C. Vitamin
D. Prosthetic group
8. The protein part of an enzyme is known as
A. Apoenzyme
B. Holoenzyme
C. Prosthetic group
D. Zymogen
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NMEICT PROJECT
9. Undefined
A. Apoenzyme = Holoenzyme + Coenzyme
B. Holoenzyme = Apoenzyme + Coenzyme
C. Coenzyme = Apoenzyme + Holoenzyme
D. Holoenzyme = Coenzyme + Apoenzyme
10. Which of the following part of enzyme is inactive and low molecular weight?
A. Apoenzyme
B. Coenzyme
C. Holoenzyme
D. None of the above
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NMEICT PROJECT
Theory module: 07 (Assignment)
INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY
Answer specifically
1. Define enzyme
2. Who coined the word enzyme
3. What is the active site of enzyme
4. What are the components of enzyme
5. What are the properties of enzyme
6. What are the classes of enzyme
7. How enzyme works
8. How enzyme activity can be measured
9. What are the units used to express enzyme activity
10. What is specific activity of enzyme
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
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NMEICT PROJECT
Theory module: 07 (References)
INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY
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Lehninger Principles of Biochemistry By David. L. Nelson and Michael. M. Cox
Outlines of Biochemistry by E.E Conn and P.K.Stump
Biochemistry by Stryer.L
Harper’s review of Biochemistry by by Martin. D. W, Mayes. P. A and Rodwell.
V. M
Practical of Biochemistry by Wilson & Walker
Microbiology by Precott, LM, Harley, JP, Klein, DA. The McGraw-Hill
Company, Sixth edition.
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NMEICT PROJECT
Theory module: 05 (Edutainment)
INTRODUCTION TO ENZYME AND BASICS OF ENZYME ASSAY
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Across
38. These enzymes carry out the
hydrolytic cleavage of C-O, C-N, C-C and
some other.:
51. biocatalyst which alters the rate of
reaction
without
undergoing
any
permanent change in themselves
71. A non protein organic substance
loosely attached to the enzyme and can
be separated by dialysis and is essential
for enzyme action.
79. _________activity, It is defined as the
amount of substrate the enzyme converts
(reactions catalyzed), per mg protein in
the enzyme preparation, per unit of time
86. This class of enzyme will carry out the
joining of two molecules coupled with
the hydrolysis of a ATP or a similar
triphosphate
56. RNAase degrade RNA molecules of
every kind irrespective of their type. Tell
the type of specificity.
1. The proteins which yields aminoacids
or their derivatives on hydrolysis are
called __________ proteins.
23. 3D structure formed by amino acids
which are being positioned far apart in
the polypeptide chain
Down
35. These are multiple forms of the given
enzyme that occurs within the same
animal species.
43. Suffix __________ should be used
only for single enzymes
51. All __________ are made up of
protein but all proteins are not made up
of _______________.
1. Absolute group _________. Ex.:
Trypsin and pepsin are proteolytic
enzymes, but they cleave peptide bonds
at specific amino acids. Tell the type of
specificity.
50. Who coins the term enzyme?
9. These class of enzymes will transfer a
group, like a methyl group or a glycosyl
group, from one compound (donor) to
another compound (acceptor)
14. James Sumner in 1926 demonstrate
that the enzyme are protein in nature by
isolating ___________ in a pure form
and crystallized it
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