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Name _______________________________________ Period ____ Date ____________________ PROTEIN FACTORY Analysis Use your protein model to complete the following information: 5 pts each 1) How many amino acids are in the protein your group synthesized? _______________ 2) The name of the protein you made is Trp-cage miniprotein. It is the smallest known protein and comes from the saliva of a Gila monster. It qualifies as a protein because it has a stable secondary structure of a helixes and bends. Below is a 3-D diagram of Trp-cage. For more info on Trp-cage visit the Protein Data Base (www.pdb.org) 3) Using your model, list the amino acids on lines below in order using the 3 letter abbreviations. 20 pts ____-____-____-____-____-____-____-____-____-____ ____-____-____-____-____-____-____-____-____-____ 4) Why is Trp-cage a protein and not just a polypeptide? (see above) At right is a DNA codon table, showing the DNA code letters for each amino acid. 5) Can more than one codon code for the same amino acid? _______ Explain. 6) Are tRNAs recycled? Explain 7) What was your job in the protein model activity? __________________________ Summarize what your job consisted of to build the protein model Use your text book and notes along with your model to complete the following: 2pts each 1) Where in the cell is the DNA located? _________________________ 2) Helicase does what to the DNA? ___________________________________ 3) Only one side of the DNA is copied. What is this side called? ____________________ 4) What is the side that is NOT copied called? _______________________________ 5) What different nitrogen base had to be used to make mRNA? ______________ 6) What is the formation of mRNA from DNA called? ___________________ 7) How many genes were used in the activity_______________________ 8) Where does the mRNA go after it is copied in nucleus? ____________________ 9) What organelle reads the mRNA code? ________________________________ 10)How many letters code for each amino acid? _____________ 11)What is each set of 3 letters called on mRNA? ______________________ 12)What molecule transports the proper amino acid to the ribosome? _____________ 13)What is the matching code sequence on the tRNA called? _________________ 14)Is tRNA reusable or only good for one time use? ___________________________ 15)What is the process of building an amino acid chain from the mRNA code called? ________ 16)What are the bonds between amino acids called? _____________________ 17)What molecule must be removed to form a peptide bond? _______ 18)Explain how a protein is made, starting with the DNA in the nucleus. 20 pts ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________ ________________________________________________________________________________