Download Cell Culture Lab Report Pro forma

PHSI 3006
ANALYSIS OF CELL PROLIFERATION IN CULTURE
Lab report pro forma
This lab report forms 1 assessment of the course. It is to be handed into the
Physiology Admin office by 4.30 pm of the day stipulated for your lab stream
(as detailed below).
Stream 1: Wed 30 May
Stream 2: Wed 6 Jun
Stream 3: Wed 13 Jun
This assessment is worth 10% of your final mark. Failure to submit your report
on time without an impairment form will result in a mark deduction of 10% per
day of the final mark.
The report is to be completed on the following sheets. Answer all
questions.
Failure to follow the pro forma will result in a zero mark allocation.
A model report will be posted on WebCT after the final deadline has passed.
This structured report form is designed to test your ability to present and
interpret data in a concise and accurate manner. Questions are designed to
help your understanding of key issues that are raised by the experiments
undertaken. The experiments should demonstrate some of the rules that
govern the normal “social” behaviour of cells.
Tips for good marks
Follow the instructions carefully. Concise means just that. Space provided is
adequate. Taking more space will not gain, but decrease, your score. All data
should be given as means (± s.d.). Failure to do so will decrease your marks.
All graphs should have appropriately labelled axes.
Continue to the next page.
Experiment 1: Cell proliferation of a somatic cell
Provide a graph of your data below. Your figure should have a concise,
descriptive accurate legend. In it you should describe concisely the effect of
time on cell number.
What did you expect to see and why?
Calculate the “doubling time” (an estimate of the time taken for completion of
1 cell cycle) of the cells in your culture.
Experiment 2: Growth requirements of cell proliferation
Provide 2 graphs of your data below. Your figures should have concise,
descriptive accurate legends. In them you should describe concisely the effect
of time with cell number. Think carefully how best to present your data (it
should be in the most informative and concise manner)
What did you expect to see and why?
Serum removal from media is a common approach to synchronising cells to
the same stage of the cell cycle. What factors might be present and which
part of the cell cycle is most likely affected?
Experiment 3: Anchorage dependent vs independent growth
What feature of growing CH-1 cells was obviously different from the normal
somatic and embryonic cell line?
On reaching confluency, what do you predict would happen to cells in cultures
of F9 and C2C12 somatic cells? Your answer should demonstrate your
reasoning.
THANK YOU.