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Abstract Submission
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2. Abstract submission deadline: June 30th, 2011
附件：摘要格式
Study of the transcriptional regulation of the unpaired3 gene
in Drosophila
Hsin-Yi Huang1 and Yu-Chen Tsai1
1
Department of Life Science, Tunghai University, Taichung, Taiwan, R.O.C.
The Janus Kinase/ Signal Transducers and Activators of Transcription (Jak/STAT)
signaling plays important roles in multiple developmental processes in Drosophila,
including embryonic segmentation, gonad development, germline stem cell renewal,
eye development, hematopoiesis, and immune response. There are three ligands, Upd
(Unpaired), Upd2 and Upd3, in Jak/STAT signaling of Drosophila. The appropriate
regulation of these ligands temporally and spatially is required to activate Jak/STAT
signaling to precisely regulate developmental processes. In this study, we will focus on
the transcriptional regulation of the upd3 gene. upd3 is expressed in eye-antenna disc in
the larval stages. The upd3 null mutant has small eye. upd3 is induced in septic injury
and oral infection in the adult stage. We will dissect the upd3 enhancers which regulate
eye development and immune response. The 19 Kb genomic region around the upd3
transcript are analyzed. These upd3 genomic fragments are cloned to enhancer-testing
vector which contains a green fluorescence protein (GFP) reporter. The enhancer-testing
constructs are injected into Drosophila and then select for transgenic lines. To
understand whether upd3 enhancers exist in these constructs, the GFP reporter will be
examined in the eye disc in the larval stages. Besides, we will investigate whether upd3
is induced while flies suffer bacteria infection or septic injury in gut in the adult flies.
We will further narrow down the possible upd3 genomic fragment. To understand
upstream signaling of the upd3 gene, we will search for the putative transcriptional
binding sites in possible upd3 genomic fragments and analyze their function in vivo.
Hopefully based on this study, we can realize the transcriptional regulation of the upd3
gene during developmental processes in Drosophila.