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Student Name: ______________________ Teacher: ______________________ District: Robeson Assessment: 9_12 Agriculture AU71 - Biotech and Agrisci Rsch I Test 4 Description: Unit H - Applied Genetics in Plants and Animals Form: 501 Date: ___________ 1. The union of haploid cells in plants is: A. B. C. D. Fertilization. Insemination. Isolation. Pollination. 2. The organ in the reproductive system of female plants and animals MOST responsible for the production and storage of haploid cells is the: A. B. C. D. Epididymus. Ovary. Ovum. Testes. 3. The removal of the male parts of a flower prior to the release of pollen is the first step in: A. B. C. D. Artificial insemination. Fertilization. Mechanical cross-pollination. Natural cross-pollination. 4. Which is MOST true of an imperfect flower? A. B. C. D. Can only be fertilized by flowers on another plant Cannot self-pollinate Cannot reproduce sexually Must self-pollinate 5. Spermatozoa are important in the sexual reproduction of animals as: A. B. C. D. A protective coating for sperm. A transportation mechanism for sperm. Specialized sperm cells capable of penetrating an egg. The haploid male cells used in reproduction. 6. The union of haploid cells occurs in the: A. B. C. D. Stigma of plants and the vagina of animals. Style of plants and the vagina of animals. Uterus of animals and the ovary of plants. Uterus of animals and the stigma of plants. 7. Progeny testing is a method of analyzing the offspring of male livestock to determine the: A. B. C. D. Breeding potential of the female. Motility and viability of the animal’s sperm. Potential for the transmission of certain traits. Presence of genetic disorders. 8. The process of fertilization offering the MOST control and requiring the least amount of semen is: A. B. C. D. Artificial insemination. Embryo transfer. Invitro-fertilization. Semen Analysis. 9. A method used during artificial insemination to produce offspring of one sex requires the use of a: A. B. C. D. Follicle stimulating hormone. Extender. Cytometer cell sorter. Motility cell sorter. 10. Which has MOST likely resulted in the greatest improvements in the genetics of production agricultural animals over the longest period of time? A. B. C. D. Antigen insertion Artificial insemination Cloning Embryo manipulation 11. Semen straws used in artificial insemination are BEST stored at: A. B. C. D. 0° F. -220° F. -320° F. -420° F. 12. Sperm cells in semen stored for artificial insemination: A. B. C. D. Maintain viability for many years in proper storage. Must be slowly thawed a few hours before use. Must be stored at 0° F to maintain viability. Should be stored under high pressure at low temperature. 13. The FIRST step in an embryo transfer procedure requires treating females with the hormone Prostaglandin to force: A. B. C. D. Estrus. Increase the motility of sperm. The release large numbers of eggs. The removal of the uterine lining. 14. The process of flushing is used in embryo transfer to: A. B. C. D. Clean the uterus of the female to be inseminated. Remove fertilized eggs from a superovulated female. Remove eggs for invitro-fertilization. Release semen from storage straws fertilizing the female. 15. Sperm sexing utilizes a laser that separates sperm based on the content of dye. Dye levels are always higher in sperm containing the: A. B. C. D. X chromosome, as female cells have more DNA. X chromosome, as male cells have more DNA. Y chromosome, as female cells have more DNA. Y chromosome, as male cells have more DNA. 16. Cloning in plants is MORE common than in animals as: A. B. C. D. Animals can only be cloned from stem cells. Animal cells cannot be extracted without killing the donor. Plants can be cloned from nearly any viable cell. Plant cells differentiate less easily. 17. A method of cloning a large number of plants with a small amount of vegetative material is: A. B. C. D. Air layering. Cellular division. Grafting. Tissue culture. 18. Livestock have recently been cloned from diploid cells removed from: A. B. C. D. Bone marrow. Mammary glands. The brain. The heart. 19. A method of quickly producing genetically identical plants and animals is: A. B. C. D. Cloning. Embryo transfer. Genetic engineering. Selective breeding. 20. Physical differences between two clones is MOST likely attributable to: A. B. C. D. Damage to DNA. DNA reversion. Environmental factors. The method of cloning. 21. The cloning of outstanding plant and animal specimens allows production agriculturalists to: A. B. C. D. Decrease the need of selective breeding to develop new varieties. Limit dependence on a single genetic strain. Quickly distribute desirable genetic traits. Quickly increase genetic diversity. 22. Alex is propagating Lillium bulbs via scale removal. He should be certain that each scale section: A. B. C. D. Contains a portion of the basal plate. Contains no remnants of the basal plate. Is less than 12cm long. Is more than 8cm long. 23. Christy is grafting a different variety of apple on one with a healthy rootstock. She is able to change varieties because, the DNA of the rootstock: A. B. C. D. Alters the DNA of the scion. Becomes recessive to the DNA of the scion. Remains distinct from the DNA of the scion. Becomes dominant to the DNA of the scion. 24. Suzzane cuts and peals the bark from a small section of the stem during air layering in order to: A. B. C. D. Girdle the stem. Increase the humidity at the cutting. Promote the formation of adventitious roots. Promote the formation of adventitious shoots. 25. Lorie achieves the HIGHEST success rates in the propagation of woody perennial plants through the use of: A. B. C. D. A gene gun. Bulblet seperation. Division. Stem cuttings. 26. If Terrance is taking herbaceous or semi-hardwood cuttings in the correct manner, he will cut the stem: A. B. C. D. Immediately below the nearest axillary bud. In the middle of a node. In the middle of an internode. Slightly above a node. 27. Abel is using meristimatic tissue culture to produce an endangered variety of pitcher plant. The last stage of the process conducted invitro is the: A. B. C. D. Division of cells and placement on shoot development media. Formation of callus material. Initiation of adventitious roots. Initiation of adventitious shoots. 28. When inserting genes into cells for the production of transgenic organisms, scientists must bypass, but not break the: A. B. C. D. Cell membrane. Cell wall. Cytoplasm. Plasma membrane. 29. Kerry is a research scientist that is developing a transgenic gene. What is the FIRST step he will have to go through in the creation of the gene? A. B. C. D. Approval by the United States Department of Defense Determination of a goal and purpose for the transformation procedure Isolation and extraction of a gene segment Successful completion of a genomic map of the species to be altered 30. If Susan is creating a transgenic organisms, her BEST source for genes is: A. B. C. D. Any organism in the same kingdom as the subject. Any plant, animal, or other living organism. Only organisms in the same species as the subject. Organisms in the same species as the subject only. 31. Heather uses a gene gun to: A. B. C. D. Insert genes into animal cells. Insert genes into plant cells. Join genes to bacteria for insertion. Remove genes from cells. 32. Prior to beginning the creation of a transgenic organism, it is important that researchers: A. B. C. D. Completely map the genome of the species to be used. Gain approval from proper regulatory agencies. Set goals and a purpose for the procedure. Successfully extract the gene segment to be inserted. 33. Linda is comfortable using transgenic organisms to transmit INSERTED genes to offspring because they transmit inserted genes: A. B. C. D. At much higher rates than naturally occurring genes. At much lower rates than naturally occurring genes. At the same rate as naturally occurring genes. Under no known circumstances. 34. Mandy is working to produce a strawberry that will withstand wet weather. In order to transfer the genes she wants in the plant, she uses a vector that she: A. B. C. D. Injects in the strawberry. Inoculates the soil with. Isolates from the strawberry plant. Puts in the same cooler with the strawberry. 35. Harvey takes a conservative approach to the use of genetic recombination. He wants to produce the MOST altered cells with the LEAST destruction of tissue, so he uses: A. B. C. D. Biolistics. Electroporation. Gene therapy. Micromanipulation. 36. Lucas is using plasmids as vectors in the process of genetic engineering. He finds plasmids to be a very good vector because they: A. B. C. D. Are uncommon in nature. Break down quickly after transferring genetic material. Insert DNA into the organisms they affect. Store large strands of DNA or even entire chromosomes.