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Using yeast genetics and systems biology to understand the origin of uncontrolled cell division. A/Prof Janni Petersen Flinders Centre for Innovation In cancer [email protected] Brief outline of Project Cancer is a disease of inappropriate cell growth and cell division. In addition, cancer cells migrate to colonise new parts of the body, here they undergo cell division in environments with limited nutrient supply and therefore cancer cells are frequently nutritionally stressed. The Target of Rapamycin (TOR) signalling pathway co-ordinates cell division with available nutrients and importantly altered TOR signalling has been linked to 80% of cancers. We exploit the simplicity of a single celled lifestyle and strong genetics in yeast to understand the principles of TOR signalling and identify key conserved regulations of this pathway, which we then subsequently study in human cells. In shedding light on the mechanisms behind environmental and TOR pathway control of cell division we will aim to target these in human cancers. The student will gain experience with a range of techniques including, yeast cell biology and genetics, Biochemistry including: SDS-PAGE, western blotting immuno-precipitations, kinase assayʼs. Molecular biology including: PCR, DNA cloning and DNA sequencing. Immunofluorescence microscopy and live cell imaging. Key references Davie, E , Forte G, and Petersen, J. Nitrogen regulates AMPK to control TORC1 signaling. Current Biology. 2015 16:445-454 Davie, E and Petersen, J. Environmental control of cell size at division. Current Opinion in Cell Biology. 2012 24:838–844 Petersen, J. TOR signalling regulates mitotic commitment through stress-activated MAPK and Polo kinase in response to nutrient stress. Biochem Soc Trans 37(1): 273-7. 2009 Petersen, J. and Nurse, P. TOR signalling regulates mitotic commitment through the stress MAP kinase pathway and the Polo and Cdc2 kinases. Nature Cell Biol. 9: 1263 – 1272. 2007