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Sal I from Streptomyces albus G Catalog Number R0754 Storage Temperature –20 °C CAS RN 81295-38-7 EC 3.1.21.4 Synonym: Restriction Endonuclease Sal I Product Description Sal I recognizes the sequence G/TCGAC and generates fragments with 5′-cohesive termini.1 Sal I generates compatible ends to Xho I. Sal I is inhibited by the presence of 5-methylcytosine at GTmCGAC and N6-methyladenine at GTCGmAC. Sal I exhibits star activity under non-optimal conditions. 100 units of Sal I can be heat inactivated after 15 minutes at 65 °C. Sal I Storage and Dilution Buffer: 10 mM Tris-HCl, 1.0 mM EDTA, 10 mM dithioerythritol, and 50% (v/v) glycerol, pH 7.5 Activity: 10,000 units/ml Cutting: 100% Unit Definition: One unit is the enzyme activity that completely cleaves 1 µg λ DNA in 1 hr. at 37 °C in a total volume of 25 µl of Buffer SH for restriction enzymes. 1 µg pBR322 DNA is digested completely by 5 units of Sal I on account of the larger number of cleavage sites per µg of pBR322 DNA as compared to λDNA. For cleavage of genomic DNA embedded in agarose, 10 units of Sal I per µg of DNA incubated for 4 hrs. at 37 °C is recommended. Digestion Buffer SH (B3657) is supplied as a 10× concentrate. Composition of 1× Digestion Buffer SH for Sal I – 100% Digestion at 37 °C: 50 mM Tris-HCl, 100 mM NaCl, 10 mM MgCl2, and 1 mM dithioerythritol, pH 7.5 Non-specific endonuclease activity: No degradation detected with >40 units for 16 hours. 1 µg of λDNA is incubated for 16 hours in 50 µl of 1× Digestion Buffer SH with excess of Sal I. Fold over digestion: 640 (40 units × 16 hrs.) Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. References 1. Arrand, J.R. et al., A new restriction endonuclease from Streptomyces albus G. J. Mol. Biol., 118, 127135 (1978) JJJ,MAM 11/12-1 2012 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.