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NMEICT PROJECT Theory module: 06(MCQ) INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS 1. For the DNA strand 5'-TACGATCATAT-3' the correct complementary DNA strand is: A. 3'-TACGATCATAT-5' B. 3'-ATGCTAGTATA-5' C. 3'-AUGCUAGUAUA-5' D. 3'-TATACTAGCAT-5' 2. All carbohydrates contain carbon, hydrogen and oxygen. All proteins contain carbon, hydrogen, oxygen and what other element A. Nitrogen B. Fluorine C. Sulphur D. Chlorine 3. B-form DNA contains------------ base pairs per helical turn of length and the length of this complete turn is---------A. 10, 3.4nm B. 10.5, 3.6nm C. 11, 4nm D. None of these 4. Palindromic DNA or RNA sequences can form: A. Hairpin B. Cruciform C. H-DNA D. 1 and 2 5. Deamination of Adenine gives, A. Hypoxanthine B. Xanthine C. Thymine D. Uracil 6. When EtBr binds DNA, the following does not occur A. : It increases the positive superhelicity of the DNA B. It stains the DNA which becomes fluorescent under UV light C. Affects the hydrodynamic properties of circular DNA D. Increases the number of turns in DNA DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 1 NMEICT PROJECT 7. Which of the following is not a characteristic of DNA? A. It has a hydroxyl group attached to the 3’ carbon at 3’ end B. It has a hydroxyl group attached to the 3’ carbon at 3’ end C. It is less susceptible to alkaline hydrolysis than RNA D. It is stabilised by hydrogen bonding, Van der Waals forces and covalent interactions 8. What is the number of hydrogen bonds in a double helical B-DNA structure of 100bp with 20 adenines and 10 thymines in one of the two strands? A. 230 B. 270 C. 300 D. 200 9. Heat denaturation of DNA includes A. Hyperchromic shift B. The higher the content of GC base pairs, the higher the melting point of the DNA C. The higher the ionic strength, the lower the melting temperature D. 1 and 2 10. The bonds in the protein structure that are not broken on denaturation A. Ionic bonds B. Peptide bonds C. Disulphide bonds D. Hydrogen bonds 11. Which is not the function of Heamoglobin A. B. C. D. It is an allosteric protein The shape of the oxygen dissociation curve of haemoglobin is sigmoidal 2, 3-Bisphosphoglycerate binds to the β-chains of deoxyhemoglobin The affinity of haemoglobin for oxygen is independent of pH DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 2 NMEICT PROJECT Theory module: 06 (Assignment) INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS Answer specifically 1. Who proposed the structure of of nucleic acid? 2. Which are the three covalently bound parts of nucleotides? 3. What are the sugars of nucleic acid? 4. Which are the bases of nucleic acid? 5. How nucleotides polymerize to form nucleotides? 6. What are the features of nucleic acid defined by Watson and Crick? 7. What are the types of DNA? 8. Which is the most common form of DNA? 9. How amino acids differs from each other? 10. How peptide bond formation takes place? 11. What is C-terminus and N-terminus? 12. What are the different levels of protein structure? 13. What are the two forms of secondary structure of protein? 14. What are the types of bond caught up in tertiary structure of protein? 15. What are the types of quaternary structure? DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 3 NMEICT PROJECT Theory module: 06 (References) INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS Lehninger Principles of Biochemistry By David. L. Nelson and Michael. M. Cox Outlines of Biochemistry by E.E Conn and P.K.Stump Biochemistry by Stryer.L Harper’s review of Biochemistry by by Martin. D. W, Mayes. P. A and Rodwell. V. M Practical of Biochemistry by Wilson & Walker Microbiology by Precott, LM, Harley, JP, Klein, DA. The McGraw-Hill Company, Sixth edition. DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 4 NMEICT PROJECT Theory module: 06 (Edutainment) INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 5 NMEICT PROJECT Across 52. Gly = ___________ aminoacid 77. An iminoacid 62. Sugar present in the nucleic acid 85. The base pairs are rotated 360 with respect to each adjacent pair, so that there are 10 pairs per helical turn and the diameter of the double helix is _____nm 17. When a colored solution absorbs light maximally at a particular wavelength then that wavelength is known as ________________ wavelength. 3. All carbohydrates contain carbon, hydrogen and oxygen. All proteins contain carbon, hydrogen, oxygen and what other element Down 46. An aminoacid that is found in the formation of disulfide bridge 42. The unique sequence of amino acids that make up a protein or polypeptide chain is called the _________ Structure 48. DNA replication is _____________ conservative. 10. The sugars and phosphates in nucleic acids are linked to each other in an alternating chain (sugar-phosphate backbone) through ___________ linkages 79. Z = _____-handed helix (found in vitro under high salt) 26. The combination of a base and sugar is called a__________. 1. A fibrous protein of tendons. 16. Spectrophotometer uses the deuterium or a ________________ lamp for uv light. 2. The proteins which yields aminoacids or their derivatives on hydrolysis are called __________ proteins. DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE CHRIST COLLEGE, RAJKOT, GUJARAT. Page 6