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NMEICT PROJECT
Theory module: 06(MCQ)
INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS
1. For the DNA strand 5'-TACGATCATAT-3' the correct complementary DNA
strand is:
A.
3'-TACGATCATAT-5'
B.
3'-ATGCTAGTATA-5'
C.
3'-AUGCUAGUAUA-5'
D.
3'-TATACTAGCAT-5'
2. All carbohydrates contain carbon, hydrogen and oxygen. All proteins contain
carbon, hydrogen, oxygen and what other element
A.
Nitrogen
B.
Fluorine
C.
Sulphur
D.
Chlorine
3. B-form DNA contains------------ base pairs per helical turn of length and the
length of this complete turn is---------A. 10, 3.4nm
B. 10.5, 3.6nm
C. 11, 4nm
D. None of these
4. Palindromic DNA or RNA sequences can form:
A. Hairpin
B. Cruciform
C. H-DNA
D. 1 and 2
5. Deamination of Adenine gives,
A. Hypoxanthine
B. Xanthine
C. Thymine
D. Uracil
6. When EtBr binds DNA, the following does not occur
A. : It increases the positive superhelicity of the DNA
B. It stains the DNA which becomes fluorescent under UV light
C. Affects the hydrodynamic properties of circular DNA
D. Increases the number of turns in DNA
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
7. Which of the following is not a characteristic of DNA?
A. It has a hydroxyl group attached to the 3’ carbon at 3’ end
B. It has a hydroxyl group attached to the 3’ carbon at 3’ end
C. It is less susceptible to alkaline hydrolysis than RNA
D. It is stabilised by hydrogen bonding, Van der Waals forces and covalent
interactions
8. What is the number of hydrogen bonds in a double helical B-DNA structure of
100bp with 20 adenines and 10 thymines in one of the two strands?
A. 230
B. 270
C. 300
D. 200
9. Heat denaturation of DNA includes
A. Hyperchromic shift
B. The higher the content of GC base pairs, the higher the melting point of the
DNA
C. The higher the ionic strength, the lower the melting temperature
D. 1 and 2
10. The bonds in the protein structure that are not broken on denaturation
A. Ionic bonds
B. Peptide bonds
C. Disulphide bonds
D. Hydrogen bonds
11. Which is not the function of Heamoglobin
A.
B.
C.
D.
It is an allosteric protein
The shape of the oxygen dissociation curve of haemoglobin is sigmoidal
2, 3-Bisphosphoglycerate binds to the β-chains of deoxyhemoglobin
The affinity of haemoglobin for oxygen is independent of pH
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Theory module: 06 (Assignment)
INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS
Answer specifically
1. Who proposed the structure of of nucleic acid?
2. Which are the three covalently bound parts of nucleotides?
3. What are the sugars of nucleic acid?
4. Which are the bases of nucleic acid?
5. How nucleotides polymerize to form nucleotides?
6. What are the features of nucleic acid defined by Watson and Crick?
7. What are the types of DNA?
8. Which is the most common form of DNA?
9. How amino acids differs from each other?
10. How peptide bond formation takes place?
11. What is C-terminus and N-terminus?
12. What are the different levels of protein structure?
13. What are the two forms of secondary structure of protein?
14. What are the types of bond caught up in tertiary structure of protein?
15. What are the types of quaternary structure?
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Theory module: 06 (References)
INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS
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Lehninger Principles of Biochemistry By David. L. Nelson and Michael. M. Cox
Outlines of Biochemistry by E.E Conn and P.K.Stump
Biochemistry by Stryer.L
Harper’s review of Biochemistry by by Martin. D. W, Mayes. P. A and Rodwell.
V. M
Practical of Biochemistry by Wilson & Walker
Microbiology by Precott, LM, Harley, JP, Klein, DA. The McGraw-Hill
Company, Sixth edition.
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Theory module: 06 (Edutainment)
INTRODUCTION TO NUCLEIC ACIDS AND PROTEINS
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Across
52. Gly = ___________ aminoacid
77. An iminoacid
62. Sugar present in the nucleic acid
85. The base pairs are rotated 360 with
respect to each adjacent pair, so that
there are 10 pairs per helical turn and the
diameter of the double helix is _____nm
17. When a colored solution absorbs light
maximally at a particular wavelength
then that wavelength is known as
________________ wavelength.
3. All carbohydrates contain carbon,
hydrogen and oxygen. All proteins
contain carbon, hydrogen, oxygen and
what other element
Down
46. An aminoacid that is found in the
formation of disulfide bridge
42. The unique sequence of amino acids
that make up a protein or polypeptide
chain is called the _________ Structure
48. DNA replication is _____________
conservative.
10. The sugars and phosphates in nucleic
acids are linked to each other in an
alternating
chain
(sugar-phosphate
backbone) through ___________ linkages
79. Z = _____-handed helix (found in
vitro under high salt)
26. The combination of a base and sugar
is called a__________.
1. A fibrous protein of tendons.
16.
Spectrophotometer
uses
the
deuterium or a ________________ lamp
for uv light.
2. The proteins which yields aminoacids
or their derivatives on hydrolysis are
called __________ proteins.
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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