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I. Intracellular Vesicular Traffic (chapter 13, Alberts) A. Two pathways: Biosynthetic secretory and endocytic, Fig. 13-1 B. The process of transport between all parts of the pathway is identical, vesicular transport, Fig. 13-2. C. Experimental Methods for studying vesicular transport 1. Cell free systems 2. Genetic 3. GFP fusion proteins D Specificity of transport- Coat formation, Fig13-5. 1. Clathrin, Fig. 13-6, Fig. 13-8 2. COPI 3. COPII E. Regulation of coat assembly 1. ARF proteins and Sar I proteins, Fig. 13-10 F. Selective traffic control 1. SNARE proteins, Fig. 13-11, Fig. 13-12 a. NSF 2. Rab proteins, Fig. 13-14 G. Membrane Fusion 1. SNARE proteins 2. Viral fusion, Fig. 13-16 II. Golgi A. Regulated transport from the ER, Fig 13-17. 1. Cystic Fibrosis 2. Vesicle tubular clusters, Fig. 13-20 3. Retrieval of ER resident proteins, Fig. 13-21 a. Kin recognition 4. Transmembrane length B. Golgi compartments, 13-22 C. Oligosaccharide chain processing 1. High mannose oligosacchrides and complex oligosacchrides, Fig. 13-26 2. O-linked glycosylation D. Proteogylcans E. Role of Glycosylation 1. Sorting 2. Protective coating 3. Binding functions 4. Regulatory F. Organization of Golgi compartments, Fig. 13-29 1. Vesicular transport vs. cisternal maturation, Fig. 13-30. III. Transport to the Lysosomes A. Role of the Lysosomes, Fig. 13-31. B. Heterogeneity of structure, Fig. 13-32 C. Plant and Fungal vacuoles D. Routes to the lysosome, Fig. 13-35 E. Protein targeting to the lysosome, the mannose 6-phosphate receptor, Fig. 1337 F. Lysosomal storage diseases 1. I cell disease, Fig. 13-38 G. Lysosomal secretion IV. Endocytosis A. Phagocytosis 1. Protozoa, macrophages, neutrophils, and dendritic cells, Fig. 13-39 2. Triggered reaction B. Pinocytosis 1. Constitutive 2. Clathrin, Fig. 13-41 3. Caveolae, Fig. 13-42 4. Receptor mediated endocytosis a. Atherosclerosis, Fig. 13-46 5. Early and late Endosomes a. Transferrin b. EGF receptors 6. Pathway to lysosomes, Fig. 13-50 7. Transcytosis, Fig. 13-51 8. Polarized cells, Fig. 13-53 V. Exocytosis A. Constitutive and regulated pathways, Fig. 13-54 B. Secretory vesicles, 13-56, 13-57 1. Protein processing 2. Storage and release C. Recycling D. Polarized cells, sorting, direct and indirect pathways 1. Lipid rafts, Fig. 13-63 E. Synaptic vesicles, Fig. 13-64