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I. Intracellular Vesicular Traffic (chapter 13, Alberts)
A. Two pathways: Biosynthetic secretory and endocytic, Fig. 13-1
B. The process of transport between all parts of the pathway is identical, vesicular
transport, Fig. 13-2.
C. Experimental Methods for studying vesicular transport
1. Cell free systems
2. Genetic
3. GFP fusion proteins
D Specificity of transport- Coat formation, Fig13-5.
1. Clathrin, Fig. 13-6, Fig. 13-8
2. COPI
3. COPII
E. Regulation of coat assembly
1. ARF proteins and Sar I proteins, Fig. 13-10
F. Selective traffic control
1. SNARE proteins, Fig. 13-11, Fig. 13-12
a. NSF
2. Rab proteins, Fig. 13-14
G. Membrane Fusion
1. SNARE proteins
2. Viral fusion, Fig. 13-16
II. Golgi
A. Regulated transport from the ER, Fig 13-17.
1. Cystic Fibrosis
2. Vesicle tubular clusters, Fig. 13-20
3. Retrieval of ER resident proteins, Fig. 13-21
a. Kin recognition
4. Transmembrane length
B. Golgi compartments, 13-22
C. Oligosaccharide chain processing
1. High mannose oligosacchrides and complex oligosacchrides, Fig. 13-26
2. O-linked glycosylation
D. Proteogylcans
E. Role of Glycosylation
1. Sorting
2. Protective coating
3. Binding functions
4. Regulatory
F. Organization of Golgi compartments, Fig. 13-29
1. Vesicular transport vs. cisternal maturation, Fig. 13-30.
III. Transport to the Lysosomes
A. Role of the Lysosomes, Fig. 13-31.
B. Heterogeneity of structure, Fig. 13-32
C. Plant and Fungal vacuoles
D. Routes to the lysosome, Fig. 13-35
E. Protein targeting to the lysosome, the mannose 6-phosphate receptor, Fig. 1337
F. Lysosomal storage diseases
1. I cell disease, Fig. 13-38
G. Lysosomal secretion
IV. Endocytosis
A. Phagocytosis
1. Protozoa, macrophages, neutrophils, and dendritic cells, Fig. 13-39
2. Triggered reaction
B. Pinocytosis
1. Constitutive
2. Clathrin, Fig. 13-41
3. Caveolae, Fig. 13-42
4. Receptor mediated endocytosis
a. Atherosclerosis, Fig. 13-46
5. Early and late Endosomes
a. Transferrin
b. EGF receptors
6. Pathway to lysosomes, Fig. 13-50
7. Transcytosis, Fig. 13-51
8. Polarized cells, Fig. 13-53
V. Exocytosis
A. Constitutive and regulated pathways, Fig. 13-54
B. Secretory vesicles, 13-56, 13-57
1. Protein processing
2. Storage and release
C. Recycling
D. Polarized cells, sorting, direct and indirect pathways
1. Lipid rafts, Fig. 13-63
E. Synaptic vesicles, Fig. 13-64