Download First Exam 2015

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BIO 447 Immunobiology
Exam 1
First Hourly Exam
I.
100 Points
October 13, 2015 Filename: 44715Xm1.doc
(20 pts) ______(Matching)
II. (16 Pts) ______447Intro
III. (16 Pts) ______ Antigens
IV. (16 Pts) ______ Antibody Structure
V. (16 Pts) ______ Interaction of Cells and Components of the Immune Response
VI. (16 Pts) ______ Videos or Photos Viewed in Class
Total : (100 Pts)
______
I. [20 Pts] Complex Matching
See page 2 for the 20 questions and 20 possible answers.
Each of the numbered items in Column One has a SINGLE BEST matching Lettered Item in Column
Two. From Column Two select the LETTER that BEST matches each NUMBERED item in Column One.
Enter that Letter in the blank next to the numbered item in Column One. Each item in Column Two can be used
only once. If you use an item more than once, it will be marked wrong wherever it appears.
You may want to enter tentative numbers in pencil first until you are sure that you have the set of
answers that you want.
Please print small so no one else can see what you are entering.
I. [20 pts]
_______Column One___________
p. 2
_______________Column Two_______________________
1. _______ Gamma globulin
A. Antigen-presenting
B. Secretory isotype of immunoglobulin; functions in mucosal linings
2. _______ Fc Regions
C. Retained immunogenicity but lost pathogenicity
3. _______ Six CDR’s
D. Universal recipient
E. PMN’s
4. _______ Dendritic Cell
F. Stimulates cell division polyclonally and non-specifically
5. _______ IP
G. A property of antibodies determined by isotype
6. ______ Mitogen
H. Hold CDR’s in the Correct Conformation
7. _______ Adjuvant
I. Can cross the placental barrier to produce hemolytic disease of the new-born (HDNB)
8. _______ Serum half-life
J. Cannot bind antigen, but can control effector functions
K. Route of immune exposure
9. _______ Induced fit
L. Can increase immunogenicity of a vaccine
10. _______ IgA
M. Conformational change in antibody triggered by antigen binding
11. ______ Myeloid lineage
N. Blood serum fraction where most of the antibodies are found
O. Based on topological and physico-chemical complementarity
12. _______ Natural Killer
P. Can polyclonally activate T-Cells
13. _______ Framework Regions
Q. Triggers a systemic cytokine storm
R. Close Together in the 3-Dimentional structure of an antibody
14. _______Lymphoid lineage
S. Cytotoxic Cells attracted by Fc Region of Antibodies
15. ______ Antigen-Antibody recognition
T. B-Cells and T-Cells
16. _______ Neisseria meningitidis endotoxin
17. _______ Louis Pasteur’s chicken cholera culture after prolonged growth
18. _______ Maternal IgG Antibody to Rh Factor
19. _______ AB Blood Group Person
20. _______Super-Antigen
p.3
II. (16 pts) 447 Intro
A.(4 Pts) The Immune response is divided into two major forms based on the manner of recognition and response to potential
pathogens and to deleterious events in the host. What are these two major forms of the Immune response?
Specific Adaptive
Innate Natural
B. (4 Pts) We cited key observations in human history that revealed important properties of immunity. What property of the
immune response is illustrated by each of these historic observations?
1, Freedom from plague and surviving the plague
Immunity Exists
2. Susceptibility to other diseases even after survival from plague
Immunity is Specific
3. Deliberately inducing an infectious disease can provide later protection
Immunity has Memory
4. A non-pathogenic variant or altered version of a pathogen can protect against the pathogen
Immunogenicity can be separated from Pathogenicity
C. (4 Pts) Give 4 Examples of types of deleterious immune response or Immunopathologies . Please give the types of such
immune-based pathologies not just example diseases
Allergy or Hypersensitivity. React in appropriately against something
Autoimmunity . React against self or have a break-down of tolerance
Transplantation Rejection. React against a cell or tissue graft that is used for therapy
Immune Responses to Infectious Diseases. Failure to respond adequately to a pathogenic challenge
Immune Deficiencies and AIDS. Congenital or acquired weakening of immune responses
Tumor Immunity: Failure to respond to a cancer challenge or promotion of cancer by the immune response
D. (4 Pts) The immune response system is radically different from organs like the heart, liver, and brain in terms of its
structural organization and its location.
In what ways is the immune response system totally different from any other system in the body except possibly the endocrine
system and the nervous system?
There are two different features about the immune response that distinguish it from other system in terms of it structural
organization.
Immune system is everywhere in the body
Immune system is discontinuous
p. 4
III. (16 Pts) With respect to Antigens, Haptens, and PAMP (Pathogen-associated Molecular Patterns)
Antigens and PAMP are targets for cells and/or soluble factors of the immune response and are recognized by
different types of membrane receptors. Antigens and PAMP differ profoundly in the types of cells that respond
to them as targets. Antigens and PAMP also differ profoundly in the type of immune response that they elicit.
A. (4 Pts) . In terms of cells or soluble factors or membrane receptors that respond:
(You need to cite only one cell type or one soluble factor or membrane receptor)
What responds to antigens?
B-Cells or T-Cells or T-Cell Receptors or Antibodies
B. (4 Pts) In terms of cells or membrane receptors that respond: (you need to cite only one cell type or one class
of membrane receptors)
What responds to PAMP?
Natural Killer Cells, or Monocytes or Macrophages or PMN’s or Neutrophils or Eosinophils or Basophils or
Mast Cells Receptors
C. (4 Pts) What type of immune response is elicited in response to antigens in question A above?
Specific Adaptive
D. (4 Pts) What type of immune response is elicited in response to PAMP in question B above?
Innate Natural
p. 5
IV. (16 pts) Antibody Structure and Use
A.(4 Pts) A single antibody molecule is “bivalent”. What does that mean in terms of antigen-antibody binding?
( 2 Pt)
One antibody protein can bind two antigenic determinants or Antibody has two Fab arms
A single antibody molecule is divided into two major regions, What are these two major regions?
(2 Pts)
Fab and Fc
B. (4 Pts) What does “CDR” stand for? ( 1 Pt) Complementarity determining region
In what region of the antibody molecule are CDR’s located ? (1 Pt)
Variable or Fab
What do CDR’s do in molecular or immunological terms?
Bind antigen or form antigen-binding site
C.(4 Pts) Give the abbreviations or names for three different full Immunoglobulin Isotypes
IgG, IgM, IgD, IgE, IgA, IgG1, IgG2, IgG3, IgG4
Gamma globulin, macroglobulin
D.(4 Pts) What is this graphic showing? Radio-active antibody labeling of metastatic cancers
or Monoclonal antibody labeling
p. 6
V. (16 Pts). Interaction of Cells and Components of the Immune Response
A. (4 Pts) What are each of these two cells doing?
The small cell on the left is a lymphocyte.
What is the big cell on the right?
T-Cell and Macrophage (also accept Dendritic Cell)
B. (4 Pt) What is the picture below showing (“Picture 1”) (2 Pts)
Clonal Selection
Picture 1
Object 1
What is the item marked “Object 1”? (2 Pt)
Antigen
p. 7
V. Continued.
C.(4 Pts) The stem cell shown in B. is making cells 1,2,3, and 4. What else does it have to make when it divides
in order to do its job correctly?
Replace itself
D. ( 4 Pts)
1.What kind of B-cell is the one at the bottom?
Plasma Cell or Antibody –secreting cell
2.What is the T-cell in the middle of the picture producing?
Cytokines (or IL2)
3. The T-cell on the right is killing a target cell. That means that the T-cell is a special kind of T-cell
called a :
Cytotoxic T-Cell or Tc
This is a special
kind of
differentiated
B-cell called
a______
cell.
What is this stuff?
What kind of T-cell is this?
p. 8
VI. (16 Pts) Videos and Photos Shown in Class
A. (4 Pts) In “Killer Disease on Campus” we saw the story of the emergency physicians from London’s St.
Mary’s Hospital going far outside of London late at night to treat a young woman, Pauline Upton, who was
gravely ill with meningococcal sepsis. One emergency care physician looked into her eyes with a light and
said “That’s good”! What was he looking for and was relieved to find that it was absent?
Brain swelling or Nervous system involvement
B. (4 Pts) In meningococcal sepsis the infectious organism is easily killed with penicillin, yet the devastating
consequence of the infection can still persist even though the organism is dying. What is causing the
persistence of the disease?
Endotoxin
C.(4 Pts) What is this photo showing? What is the green structure on the left?
What are the blue and orange structures on the right?
p. 9
D.(4 Pts) What pathology is this little boy exhibiting? Smallpox