The Role of the Innate and Adaptive Immune Responses in
... the trophozoite to the corneal epithelium. The ability of the parasite to bind to the corneal epithelium is believed to be a prerequisite for the establishment of Acanthamoeba keratitis. For example, there is considerable variability in the capacity of Acanthamoeba trophozoites to bind to the cornea ...
... the trophozoite to the corneal epithelium. The ability of the parasite to bind to the corneal epithelium is believed to be a prerequisite for the establishment of Acanthamoeba keratitis. For example, there is considerable variability in the capacity of Acanthamoeba trophozoites to bind to the cornea ...
Pemphigoid diseases: Pathogenesis, diagnosis, and treatment
... domain of BP180, which are recognized by IgE and IgG autoantibodies in BP patients [16 –18,31 – 34]. BP patients also exhibit significant reactivity with BP230, which is thought not to be involved in the initiation of the inflammatory response in BP due to its intracellular localization [8,35 –40]. ...
... domain of BP180, which are recognized by IgE and IgG autoantibodies in BP patients [16 –18,31 – 34]. BP patients also exhibit significant reactivity with BP230, which is thought not to be involved in the initiation of the inflammatory response in BP due to its intracellular localization [8,35 –40]. ...
Humoral and cellular immune responses to Helicobacter
... findings have emphasized the importance of the adherence factors for the induction of gastric inflammation, ulcer disease, and also gastric adenocarcinoma. It has been found that bacterial colonization densities are of great importance for the degree of mucosal inflammation and damage, (10, 56, 151) ...
... findings have emphasized the importance of the adherence factors for the induction of gastric inflammation, ulcer disease, and also gastric adenocarcinoma. It has been found that bacterial colonization densities are of great importance for the degree of mucosal inflammation and damage, (10, 56, 151) ...
Novel tests for diagnosing tuberculous pleural effusion: what works and what REVIEW
... T-cell response to specific antigens Recently, in vitro, T-cell-based IFN-c release assays (IGRAs) have been developed and licensed for diagnosis of latent TB infection. Normally, these tests use peripheral blood mononuclear cells (PBMCs), but they can be used with pleural fluid mononuclear cells. T ...
... T-cell response to specific antigens Recently, in vitro, T-cell-based IFN-c release assays (IGRAs) have been developed and licensed for diagnosis of latent TB infection. Normally, these tests use peripheral blood mononuclear cells (PBMCs), but they can be used with pleural fluid mononuclear cells. T ...
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... several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of n ...
... several new applications for oligopeptide phage display in disease related fields have been developed which has led to the identification of various new antigens. These novel identified immunogenic proteins provide new insights into host pathogen interactions and can be used for the development of n ...
Nail Disorders - domenicoscience
... Indiana, as of May 1, 1999, 23 states have banned this chemical from being used in nail salons. MMA nails are very rigid and do not bend or have the flexibility to break. When MMA does finally break, it will break the natural nail with it, causing severe nail damage. ...
... Indiana, as of May 1, 1999, 23 states have banned this chemical from being used in nail salons. MMA nails are very rigid and do not bend or have the flexibility to break. When MMA does finally break, it will break the natural nail with it, causing severe nail damage. ...
1 accounts for 30%
... Absorption of Anti-Peptide 43-88 with BP. In order to remove antibodies to intact BP, a BPimmunoadsorbent was prepared. 1 g ofAH Sepharose 4B was washed with 300 ml 0.5 M NaC1 and 100 ml distilled HsO after which it was suspended in 10 ml distilled H20.15 mg each of bovine BP microheterogeneous comp ...
... Absorption of Anti-Peptide 43-88 with BP. In order to remove antibodies to intact BP, a BPimmunoadsorbent was prepared. 1 g ofAH Sepharose 4B was washed with 300 ml 0.5 M NaC1 and 100 ml distilled HsO after which it was suspended in 10 ml distilled H20.15 mg each of bovine BP microheterogeneous comp ...
THE HLA SYSTEM
... By this method, the DNA for a whole region (eg the HLA DR gene region) is amplified in the PCR. The amplified DNA is then tested by adding labelled (eg Radioactive) oligonucleotide probes, which are complementary for DNA sequences, characteristic for certain HLA antigens. These probes will then "typ ...
... By this method, the DNA for a whole region (eg the HLA DR gene region) is amplified in the PCR. The amplified DNA is then tested by adding labelled (eg Radioactive) oligonucleotide probes, which are complementary for DNA sequences, characteristic for certain HLA antigens. These probes will then "typ ...
The Origins, Specificity, and Potential Biological
... characterized anti-immunoglobulin autoantibodies are those directed against the Fc region of IgG, known as rheumatoid factor, or those directed against the variable regions, known as anti-idiotype autoantibodies. Over the years, another class of anti-immunoglobulin autoantibody has been described: t ...
... characterized anti-immunoglobulin autoantibodies are those directed against the Fc region of IgG, known as rheumatoid factor, or those directed against the variable regions, known as anti-idiotype autoantibodies. Over the years, another class of anti-immunoglobulin autoantibody has been described: t ...
Prolonged suckling period in organic piglet production – Effects on
... During weaning, piglets are under strain from the loss of their dam, the change in feed, and a new microbiological environment. How much this strain influences the piglets depends mostly on their immune system. Piglets from organic production are weaned later (at least 40 days) than piglets from con ...
... During weaning, piglets are under strain from the loss of their dam, the change in feed, and a new microbiological environment. How much this strain influences the piglets depends mostly on their immune system. Piglets from organic production are weaned later (at least 40 days) than piglets from con ...
Ronald van Ree
... A high-dose protective effect as observed for cat has so far not been found for house dust mite. For food allergens this is even more debated. The outcome has very significant public health impact. “Promote cats and peanut butter sandwiches early on or not?” ...
... A high-dose protective effect as observed for cat has so far not been found for house dust mite. For food allergens this is even more debated. The outcome has very significant public health impact. “Promote cats and peanut butter sandwiches early on or not?” ...
An Introduction to Artificial Immune Systems
... determine its stimulation level (based on antigenic and network interaction) 2.2 Metadynamics: eliminate network cells with a low stimulation 2.3 Clonal Expansion: select the most stimulated network cells and reproduce them proportionally to their stimulation 2.4 Somatic hypermutation: mutate each c ...
... determine its stimulation level (based on antigenic and network interaction) 2.2 Metadynamics: eliminate network cells with a low stimulation 2.3 Clonal Expansion: select the most stimulated network cells and reproduce them proportionally to their stimulation 2.4 Somatic hypermutation: mutate each c ...
... Immunity to asexual blood-stage of Plasmodium falciparum malaria is believed to be associated with protective antibodies of certain immunoglobulin classes and subclasses. This thesis addressed the importance of antibodies in relation to malaria infection and their effective interactions with Fc gamm ...
The age-dependent expression of the F18 E. coli receptor
... (anti-H-2 mAb (13 mg/ml; clone 92FR-A2; DAKO), anti-A-2 mAb (59 mg/ml; clone 29.1; Sigma–Aldrich) or anti-CD174 (1/7.5 diluted; clone A70-C/C8; Serotec)) diluted in PBS and with FITC-conjugated secondary anti-mouse antibody during 1 h at 37 8C. PI-staining was performed to visualize cell nuclei. The ...
... (anti-H-2 mAb (13 mg/ml; clone 92FR-A2; DAKO), anti-A-2 mAb (59 mg/ml; clone 29.1; Sigma–Aldrich) or anti-CD174 (1/7.5 diluted; clone A70-C/C8; Serotec)) diluted in PBS and with FITC-conjugated secondary anti-mouse antibody during 1 h at 37 8C. PI-staining was performed to visualize cell nuclei. The ...
Immunology and Evolution of Infectious Disease
... Rather, I have long been interested in how the molecular basis of recognition between attackers and defenders sets the temporal and spatial scale of the battle. Attack and defense occur between insects and the plants they eat, between fungi and the crop plants they destroy, between viruses and the b ...
... Rather, I have long been interested in how the molecular basis of recognition between attackers and defenders sets the temporal and spatial scale of the battle. Attack and defense occur between insects and the plants they eat, between fungi and the crop plants they destroy, between viruses and the b ...
The Role of Donor‐Specific HLA Alloantibodies in Liver
... DSA. However, the high sensitivity of SPIs increases their susceptibility to environmental variables and interference by substances in serum (9). For these reasons, a cell-based assay is frequently paired with an SPI that utilizes purified Class I and/or II HLA antigens as targets, collectively enab ...
... DSA. However, the high sensitivity of SPIs increases their susceptibility to environmental variables and interference by substances in serum (9). For these reasons, a cell-based assay is frequently paired with an SPI that utilizes purified Class I and/or II HLA antigens as targets, collectively enab ...
Full-Text PDF
... of Ovarian Cancer Screening, UKCTOCS, www.ukctocs.org) in a multimodality approach, estimates a woman’s risk of ovarian cancer (ROC) [6]. Here, an estimate on the basis of age and modified by the relative fit of the serial CA125 profile to the change-point model estimated from known cases is compare ...
... of Ovarian Cancer Screening, UKCTOCS, www.ukctocs.org) in a multimodality approach, estimates a woman’s risk of ovarian cancer (ROC) [6]. Here, an estimate on the basis of age and modified by the relative fit of the serial CA125 profile to the change-point model estimated from known cases is compare ...
host susceptibility to rotavirus infection and
... fragments (secreted or anchored) were shown to bind to a broad range of human rotavirus serotypes in vitro. In Paper V, we have shown that the fusion of the mouse IgG1 Fc to ARP1 (Fc-ARP1) confers a markedly increased protection against rotavirus in a neonatal mouse model of rotavirus-induced diarrh ...
... fragments (secreted or anchored) were shown to bind to a broad range of human rotavirus serotypes in vitro. In Paper V, we have shown that the fusion of the mouse IgG1 Fc to ARP1 (Fc-ARP1) confers a markedly increased protection against rotavirus in a neonatal mouse model of rotavirus-induced diarrh ...
Immunotolerance against a foreign antigen transgenically
... Antibody levels were measured by enzyme-linked imraunosorbent assay (ELISA).l4 Wells were coated with 300 ng HEL, and bound antibody was detected by peroxidase-conjugated goat anti-mouse IgG GCN, Aurora, OH). The data are presented as optical density absorbance at 405nm. ...
... Antibody levels were measured by enzyme-linked imraunosorbent assay (ELISA).l4 Wells were coated with 300 ng HEL, and bound antibody was detected by peroxidase-conjugated goat anti-mouse IgG GCN, Aurora, OH). The data are presented as optical density absorbance at 405nm. ...
Immunity to Intracellular Salmonella Depends on Surface
... Invasive Salmonella infection is an important health problem that is worsening because of rising antimicrobial resistance and changing Salmonella serovar spectrum. Novel vaccines with broad serovar coverage are needed, but suitable protective antigens remain largely unknown. Here, we tested 37 broad ...
... Invasive Salmonella infection is an important health problem that is worsening because of rising antimicrobial resistance and changing Salmonella serovar spectrum. Novel vaccines with broad serovar coverage are needed, but suitable protective antigens remain largely unknown. Here, we tested 37 broad ...
Characterizing the immune response to HIV-1 using host derived epitope R7V
... Full name: __________________________________________________ ____________________________ ...
... Full name: __________________________________________________ ____________________________ ...
- Archives of Razi Institute
... with Toxoplasma gondii (Tehran strain). In this study, 28 healthy kittens aged 1.5 – 2 months old divided randomly into 4 groups of seven. In group 1, Kittens were fed with the infected brain tissues of mice, all of seven kittens (100 %), shed oocyst, nearly 1 week after infection, which lasted for ...
... with Toxoplasma gondii (Tehran strain). In this study, 28 healthy kittens aged 1.5 – 2 months old divided randomly into 4 groups of seven. In group 1, Kittens were fed with the infected brain tissues of mice, all of seven kittens (100 %), shed oocyst, nearly 1 week after infection, which lasted for ...
Transmission-Blocking Immunity against Malaria: From Antigen
... (infectivity reduced up to 96.2-96.6%).40 This is quite different for some other antigens. For example, TBV25H secreted by Saccharomyces cerevisiae is the recombinant type of the molecules tested in the human phase I clinical trials (D. C. Kaslow et al., unpublished data). Vaccination of mammals wit ...
... (infectivity reduced up to 96.2-96.6%).40 This is quite different for some other antigens. For example, TBV25H secreted by Saccharomyces cerevisiae is the recombinant type of the molecules tested in the human phase I clinical trials (D. C. Kaslow et al., unpublished data). Vaccination of mammals wit ...
Fulltext PDF - Indian Academy of Sciences
... E. histolytica and E. dispar while the other four reacted only with E. histolytica (Petri et al 1990a). These antibodies are the basis of two kits manufactured by TechLab Inc. (www.Techlab.com) one of which (based on one of the non-specific monoclonal antibodies) identifies E. histolytica/E. dispar ...
... E. histolytica and E. dispar while the other four reacted only with E. histolytica (Petri et al 1990a). These antibodies are the basis of two kits manufactured by TechLab Inc. (www.Techlab.com) one of which (based on one of the non-specific monoclonal antibodies) identifies E. histolytica/E. dispar ...
Generation of Monoclonal Antibodies to Cryptic Collagen Sites by
... within the intact collagen molecule. Thus, establishment of a protocol to shift the immune response away from the immu-nodominant epitopes toward cryptic sites that are only exposed following proteolysis would be of great use. To this end, we have utilized a unique immunological tolerization approac ...
... within the intact collagen molecule. Thus, establishment of a protocol to shift the immune response away from the immu-nodominant epitopes toward cryptic sites that are only exposed following proteolysis would be of great use. To this end, we have utilized a unique immunological tolerization approac ...
ELISA
The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance.ELISA is a popular format of ""wet-lab"" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a ""sandwich"" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Of note, ELISA can perform other forms of ligand binding assays instead of strictly ""immuno"" assays, though the name carried the original ""immuno"" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or ""immunosorbed"" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, and so are not easily reusable.