The potential protective immune responses to synthetic peptides
... Summary. The immunodominant and T-cell epitopes within the fimbrial subunit protein (fimbrilin) of Porphyromonas gingivalis strain 38 1 were analysed by multi-pin peptide synthesis technology. Six regions with immunodominant epitopes within a sequence of 337 amino acids that reacted with the serum o ...
... Summary. The immunodominant and T-cell epitopes within the fimbrial subunit protein (fimbrilin) of Porphyromonas gingivalis strain 38 1 were analysed by multi-pin peptide synthesis technology. Six regions with immunodominant epitopes within a sequence of 337 amino acids that reacted with the serum o ...
the cd40 receptor - Immunotechnology
... antibodies, and today the number of monoclonal antibodies in different stages of clinical trials are increasing rapidly (Borrebaeck and Carlsson, 2001). Selection of antibodies from a phage display library mimics the adaptive immune response in that it relies on clonal selection and amplification of ...
... antibodies, and today the number of monoclonal antibodies in different stages of clinical trials are increasing rapidly (Borrebaeck and Carlsson, 2001). Selection of antibodies from a phage display library mimics the adaptive immune response in that it relies on clonal selection and amplification of ...
dengue in and around nagpur
... established between the platelet count or bleeding manifestations and hence it indicated other features contributing to the bleeding diatheses. Since no platelet function tests or coagulation profile was available, the exact cause could not be elucidated. Dengue specific IgM antibodies were found in ...
... established between the platelet count or bleeding manifestations and hence it indicated other features contributing to the bleeding diatheses. Since no platelet function tests or coagulation profile was available, the exact cause could not be elucidated. Dengue specific IgM antibodies were found in ...
comparative characterization of porins from salmonella typhio
... perfusion through a sepharose-48 column (2.5 x 79.5 cm) ...
... perfusion through a sepharose-48 column (2.5 x 79.5 cm) ...
COMMITTEE FOR MEDICINAL PRODUCTS FOR HUMAN USE (CHMP)
... emerged over the past years against infectious, allergic and autoimmune diseases and also for cancer and fertility treatment. In many cases, because of their low immunogenicity these vaccines require adjuvants. New technologies in the fields of analytical biochemistry, macromolecular purification, r ...
... emerged over the past years against infectious, allergic and autoimmune diseases and also for cancer and fertility treatment. In many cases, because of their low immunogenicity these vaccines require adjuvants. New technologies in the fields of analytical biochemistry, macromolecular purification, r ...
Drug-induced immune thrombocytopenia
... has not been directly validated, but is consistent with studies showing that quinine is ÔtrappedÕ on the platelet surface when antibody binds [22] and with the finding that quinine-dependent, platelet-reactive monoclonal antibodies bind drug at the 1 : 2 ratio expected for a specific antibody–drug int ...
... has not been directly validated, but is consistent with studies showing that quinine is ÔtrappedÕ on the platelet surface when antibody binds [22] and with the finding that quinine-dependent, platelet-reactive monoclonal antibodies bind drug at the 1 : 2 ratio expected for a specific antibody–drug int ...
Chemical basis of ABO subgroups
... For the next 20 years a wave of ABO gene discovery continued and by 2009 a total of 215 ABO alleles had been reported [8], and new alleles still continue to be identified and reported [9]. ...
... For the next 20 years a wave of ABO gene discovery continued and by 2009 a total of 215 ABO alleles had been reported [8], and new alleles still continue to be identified and reported [9]. ...
Vaccination with recombinant fusion proteins incorporating Toll
... as previously described [25]. For direct ELISAs, 96 well plates were coated with OVA (Sigma–Aldrich) at 2 g/ml in PBS. Wells were then blocked with Assay Diluent Buffer (BD, PharMingen) and incubated with serial dilutions of immune sera for 1 h at room temperature. Plates were then washed 3× with P ...
... as previously described [25]. For direct ELISAs, 96 well plates were coated with OVA (Sigma–Aldrich) at 2 g/ml in PBS. Wells were then blocked with Assay Diluent Buffer (BD, PharMingen) and incubated with serial dilutions of immune sera for 1 h at room temperature. Plates were then washed 3× with P ...
Brucellosis in Animals - Cairo University Scholars
... conclusive if +, but not when 2. Detection of antibodies which is conclusive if - , but it is not 100% conclusive when +(false +, false -) ...
... conclusive if +, but not when 2. Detection of antibodies which is conclusive if - , but it is not 100% conclusive when +(false +, false -) ...
Immunogenicity - Biologics and Biosimilars
... To have sufficient sensitivity and specificity for non GLP Be validated for GLP studies Neutralizing Abs are typically not done in non-human primates but is considered in a case by case basis and depends upon a variety of factors that include: Type of drug and MOA ADA incidence Toxicity PK d ...
... To have sufficient sensitivity and specificity for non GLP Be validated for GLP studies Neutralizing Abs are typically not done in non-human primates but is considered in a case by case basis and depends upon a variety of factors that include: Type of drug and MOA ADA incidence Toxicity PK d ...
Establishment of immunohistochemical double staining
... detecting biomarkers such as PD1 and PDL1 are necessary since the anti PD1 and anti PDL1 drugs already have been approved for different cancer forms in the U.S. Issues with creating standardized, reliab ...
... detecting biomarkers such as PD1 and PDL1 are necessary since the anti PD1 and anti PDL1 drugs already have been approved for different cancer forms in the U.S. Issues with creating standardized, reliab ...
Monoclonal Antibodies for Therapeutic Use: Specific Characteristics
... is based on the following points: – therapeutic efficacy is sometimes spectacular. An action on key players in the pathophysiology of disease is possible; – knowledge of potential targets is rapidly growing. The number of antigens that can be targeted by MoAb is potentially infinite; the only limita ...
... is based on the following points: – therapeutic efficacy is sometimes spectacular. An action on key players in the pathophysiology of disease is possible; – knowledge of potential targets is rapidly growing. The number of antigens that can be targeted by MoAb is potentially infinite; the only limita ...
High salivary secretory IgA antibody levels are sensitized infants
... 67). Twenty-two children reported wheezing both at one and four years (persistent wheezing) and 26 children only at four years (late-onset wheezing). High levels of SIgA (in the upper quartile) were associated with less late-onset wheezing in sensitized children. In children with a positive SPT at f ...
... 67). Twenty-two children reported wheezing both at one and four years (persistent wheezing) and 26 children only at four years (late-onset wheezing). High levels of SIgA (in the upper quartile) were associated with less late-onset wheezing in sensitized children. In children with a positive SPT at f ...
Cervarix-H-C-721-P46-0088: EPAR - EMA
... HPV-071 is an ongoing Phase IIIb observer-blind, randomized, multi-centre trial (Sweden, Hong Kong, France and Singapore). ...
... HPV-071 is an ongoing Phase IIIb observer-blind, randomized, multi-centre trial (Sweden, Hong Kong, France and Singapore). ...
Original Research Article
... resulting in transient hyperthyroidism. These antibodies can even lyse the thyroid cells. B cells present the thyroid antigen to T cells. T cells secrete cytokines which activate a variety of other immune cells, and has a role in antibody production (Th2 cells) and apoptotic destruction of thyroid c ...
... resulting in transient hyperthyroidism. These antibodies can even lyse the thyroid cells. B cells present the thyroid antigen to T cells. T cells secrete cytokines which activate a variety of other immune cells, and has a role in antibody production (Th2 cells) and apoptotic destruction of thyroid c ...
Seroprevalence of Mycoplasma pneumoniae in HIV
... Kleemola et al., 1982). We tested a single serum sample from each patient, as this has been reported to be of epidemiologic value and helps in determining the percentage of a population that has actually been exposed to the organism (Velleca et al., 1980). In addition, the complement fixation test ( ...
... Kleemola et al., 1982). We tested a single serum sample from each patient, as this has been reported to be of epidemiologic value and helps in determining the percentage of a population that has actually been exposed to the organism (Velleca et al., 1980). In addition, the complement fixation test ( ...
A Case of Cutaneous Penicilliosis in a Child with Acute Myeloid
... infection. A broncho-alveolar lavage (BAL) was processed and was positive for Galactomannan antigen (the exact levels were not available to us) although the fungal cultures of the BAL fluid were negative. He was thus diagnosed with probable pulmonary Aspergillosis and was initially treated with amph ...
... infection. A broncho-alveolar lavage (BAL) was processed and was positive for Galactomannan antigen (the exact levels were not available to us) although the fungal cultures of the BAL fluid were negative. He was thus diagnosed with probable pulmonary Aspergillosis and was initially treated with amph ...
production and quality control of monoclonal antibodies
... An alternative approach to circumvent the HAMA response, the limited duration of effective murine antibody therapy and several manufacturing problems in the production of human monoclonal antibodies is the production of so called chimeric and humanised monoclonal antibodies using recombinant DNA (rD ...
... An alternative approach to circumvent the HAMA response, the limited duration of effective murine antibody therapy and several manufacturing problems in the production of human monoclonal antibodies is the production of so called chimeric and humanised monoclonal antibodies using recombinant DNA (rD ...
Enzyme Support for Your Digestive Health
... – Lactase is the main enzyme required to break down dairy based products. • Lactose intolerance is common worldwide. • Often, people stop producing the enzyme lactase between the ages of three and seven, making dairy consumption difficult for the rest of their lives. • In most cases, problems digest ...
... – Lactase is the main enzyme required to break down dairy based products. • Lactose intolerance is common worldwide. • Often, people stop producing the enzyme lactase between the ages of three and seven, making dairy consumption difficult for the rest of their lives. • In most cases, problems digest ...
Frequent and seasonally variable sublethal anthrax infections are
... perspective, particularly in the context of seasonal and longitudinal dynamics. In addition, though most ecological immunology studies employ serological antibody assays, endpoint titre determination is usually based on subjective criteria and needs to be made more objective. 2. Despite the fact tha ...
... perspective, particularly in the context of seasonal and longitudinal dynamics. In addition, though most ecological immunology studies employ serological antibody assays, endpoint titre determination is usually based on subjective criteria and needs to be made more objective. 2. Despite the fact tha ...
- Royal College of Surgeons
... presentation which forms the same process as the process by which bacteria and viruses are presented to the immune system. But you will see on the other side of the slide the direct allorecognition, this is the reason why the HLA system is a major barrier to transplantation. Here we have a setting w ...
... presentation which forms the same process as the process by which bacteria and viruses are presented to the immune system. But you will see on the other side of the slide the direct allorecognition, this is the reason why the HLA system is a major barrier to transplantation. Here we have a setting w ...
Effect of Boar Seminal Immunosuppressive Fraction on B
... The significance of differences between experimental and control groups was analyzed by means of Student's ttest. ...
... The significance of differences between experimental and control groups was analyzed by means of Student's ttest. ...
full text pdf
... are intended for parenteral administration. This not only results in poor patient compliance but is also unable to elicit mucosal immunity, eliciting only a systemic antibody response, which is often insufficient to tackle pathogens that employ mucosal surfaces for ingress into the host or which res ...
... are intended for parenteral administration. This not only results in poor patient compliance but is also unable to elicit mucosal immunity, eliciting only a systemic antibody response, which is often insufficient to tackle pathogens that employ mucosal surfaces for ingress into the host or which res ...
Cryptic T-Cell Epitopes and their Role in the
... Thyroid peroxidase (TPO). This is a known target autoantigen in Graves' disease. To analyse TPOspeci®c T cells, polyclonal stimulators (anti-CD3 monoclonal antibody or phytohaemagglutinin) were used to clone from Graves' thyroid in®ltrates in the absence of antigen stimulation [12]. Of two clones wh ...
... Thyroid peroxidase (TPO). This is a known target autoantigen in Graves' disease. To analyse TPOspeci®c T cells, polyclonal stimulators (anti-CD3 monoclonal antibody or phytohaemagglutinin) were used to clone from Graves' thyroid in®ltrates in the absence of antigen stimulation [12]. Of two clones wh ...
ELISA
The enzyme-linked immunosorbent assay (ELISA) (/ɨˈlaɪzə/, /ˌiːˈlaɪzə/) is a test that uses antibodies and color change to identify a substance.ELISA is a popular format of ""wet-lab"" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample.The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries.Antigens from the sample are attached to a surface. Then, a further specific antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and, in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change in the substrate.Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a ""sandwich"" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme, or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample.Of note, ELISA can perform other forms of ligand binding assays instead of strictly ""immuno"" assays, though the name carried the original ""immuno"" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or ""immunosorbed"" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g. a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase which is part of the plate, and so are not easily reusable.