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Transcript
Plant and Animal Cell Lab
1. List the 3 parts of the Cell Theory
_____________________________________
_____________________________________
_____________________________________
2. Describe or define each of the following
cell membrane _____________________________
cytoplasm _________________________________
nucleus ___________________________________
organelle ________________________________
Part A. Cheek Cells
Procedure:
1. Put a drop of methylene blue on a slide. Caution: methylene blue will stain clothes and skin.
2. Gently scrape the inside of your cheek with the flat side of a toothpick. Scrape lightly.
3. Stir the end of the toothpick in the stain and throw the toothpick away.
4. Place a coverslip onto the slide
5. View under low, medium, and high power. Cells should be visible, but they will be small and look like nearly clear
purplish blobs. If you are looking at something very dark purple, it is probably not a cell
7. Once you think you have located a cell, switch to high power and refocus. (Remember, do NOT use the coarse
adjustment knob at this point)
*You are looking for light colored blobs with dark spots in them. Perfect circles with black outlines are air bubbles.
3. Sketch the cell at low and high power. Label the nucleus, cytoplasm, and cell membrane. Draw your cells to scale.
LOW
HIGH
Part B: Onion Cells
1.
2.
3.
4.
5.
Get a slice of onion from the teacher.
Peel of a thin slice and put it on the slide.
Put a drop of methylene blue on the onion slice and put a cover slip over it.
View under low, medium and high power. Sketch cells at each magnification.
Label cell wall, nucleus, cytoplasm, and anything else you see.
LOW
MEDIUM
HIGH
Part C: Elodea Cells
1. Get a leaf of Elodea.
2. Place it on the slide.
3. Put a drop of water on it and place a cover slip over it.
4. View under low, medium and high power. Sketch cells at each magnification.
5. Label cell wall, nucleus, chloroplasts, cytoplasm, vacuole, and anything else you see.
LOW
MEDIUM
HIGH
Post Lab Questions
1. Why is methylene blue necessary?
2. The light microscope used in the lab is not powerful enough to view other organelles in the cheek cell. What parts of
the cell were visible?
3. List 2 organelles that were NOT visible but should have been in the cheek cell.
4. Is the cheek cell a eukaryote or prokaryote? How do you know?
5. Keeping in mind that the mouth is the first site of chemical digestion in a human. Your saliva starts the process of
breaking down the food you eat. Keeping this in mind, what organelle do you think would be numerous inside the cells
of your mouth?
6. What is the function of chloroplasts?
7. Name two structures found in plant cells but not animal cells.
8. Name three structures found in plant cells AND animal cells.
9. What structure surrounds the cell membrane (in plants) and gives the cell support.
10. Describe the shape and the location of chloroplasts.
11. Why were no chloroplasts found in the onion cells? (hint: think about where you find onions)
12. Which type of cell was smaller - the onion cells or the elodea cells?
13. Create a Venn Diagram below to show the differences and similarities between the onion cells and the elodea cells.