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Name ______________________________________________ Period ____________ Date ________________ Plant Cell Lab Purpose: Students will observe plant cells using a light microscope. Two cells will be observed, one from the skin of an onion, and the other from a common aquarium water plant (anacharis). Students will compare both types of cells. See also: Plant Cell Lab Makeup, which utilizes web resources to complete lab guide. Prelab Questions 1. What is the function of chloroplasts? _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 2. Name two structures found in plant cells but not animal cells. _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 3. Name three structures found in plant cells AND in animal cells. _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 4. What structure surrounds the cell membrane (in plants) and gives the cell support. _________________________________________________________________________________________ _________________________________________________________________________________________ 1 Part A - Onion Cells Obtain a prepared slide of onion cells or prepare one yourself. View under the microscope and sketch the cells at each magnification. Label the cells as they appear under high power. Part B - Elodea Cells View a prepared slide of elodea (anacharis), which is an aquarium plant. As the slide warms from the light of the microscope, you may see the chloroplasts moving, a process called cytoplasmic streaming. Post Lab Questions 1. Describe the shape and the location of chloroplasts. _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 2 2. Why were no chloroplasts found in the onion cells? (hint: think about where you find onions) _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 3. Which type of cell was smaller - the onion cells or the elodea cells? _________________________________________________________________________________________ _________________________________________________________________________________________ _________________________________________________________________________________________ 4. Fill out the Venn diagram below to show the differences and similarities between the onion cells and the elodea cells. Onion Cells Elodea Cells 3 The Human Cheek Cell Procedure: 1. Put a drop of methylene blue on a slide. CAUTION: methylene blue will stain clothes and skin. 2. Gently scrape the inside of your cheek with the flat side of a toothpick. Scrape lightly. 3. Stir the end of the toothpick in the stain and throw the toothpick away. 4. Place a coverslip onto the slide 5. Use the SCANNING objective to focus. You probably will not see the cells at this power. 6. Switch to low power. Cells should be visible, but they will be small and look like nearly clear purplish blobs. If you are looking at something very dark purple, it is probably not a cell 7. Once you think you have located a cell, switch to high power and refocus. (Remember, do NOT use the coarse adjustment knob at this point) 1. List the 3 parts of the Cell Theory 1. _______________________________________________________________________________________ 2. _______________________________________________________________________________________ 3. _______________________________________________________________________________________ 2. Describe or define each of the following --cell membrane: _____________________________________________________________________ --cytoplasm ___________________________________________________________________________ --nucleus ______________________________________________________________________________ --organelle ____________________________________________________________________________ 4 3. Sketch the cell at low and high power. Label the nucleus, cytoplasm, and cell membrane of a single cell. Draw your cells to scale. Low Power High Power 5. Why is methylene blue necessary? _________________________________________________________________________________________________ _________________________________________________________________________________________________ 6. The light microscope used in the lab is not powerful enough to view other organelles in the cheek cell. What parts of the cell were visible? _________________________________________________________________________________________________ _________________________________________________________________________________________________ 6. List 2 organelles that were NOT visible but should have been in the cheek cell. _________________________________________________________________________________________________ _________________________________________________________________________________________________ 7. Is the cheek cell a eukaryote or prokaryote? How do you know? _________________________________________________________________________________________________ _________________________________________________________________________________________________ 5 8. Keeping in mind that the mouth is the first site of chemical digestion in a human. Your saliva starts the process of breaking down the food you eat. Keeping this in mind, what organelle do you think would be numerous inside the cells of your mouth? _________________________________________________________________________________________________ _________________________________________________________________________________________________ _________________________________________________________________________________________________ 6