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Bryan Richards - Lesson Plan
Course/Class: Science 10
Topic: Cell Structures and Functions
Name: Bryan Richards (1399346)
Unit: C: Cycling of Matter in
Living Systems
Date: 30/09/2014
Grade: 10
A. Program of Studies Learning Outcomes
Science 10 – Unit C – STS and Knowledge
Students will:
2. “Describe the function of cell organelles and structures in a cell, in terms of life
processes, and use models to explain these processes and their applications
 identify the structure and describe, in general terms, the function of the cell
membrane, nucleus, lysosome, vacuole, mitochondrion, endoplasmic
reticulum, Golgi apparatus, ribosomes, chloroplast and cell wall, where
present, of plant and animal cells”
B. Lesson Purpose or Focus Question
What is the purpose of the nucleus?
C. Lesson/Instructional Outcomes
Students can identify the purpose of the nucleus in plant and animal cells
D. Activities
Lesson Introduction
 Recap on major structures of plant and animal cells
o What structures do they have in common?
o What structures are specific to plant or animal cells?
 Recap on the nucleus of a cell
o What does it contain?
 Inform students of the demonstration for the day (extracting DNA
from Strawberries)
o What would we have to do to the cell and the nucleus in
order to extract DNA?
Lesson Body
 SEE Strawberry DNA Extraction Experiment – Perform
o Step 2
 Why do you think the soap detergent is important?
 What does soap when cleaning dishes?
Especially for removing oils or fatty
substances? (aids at removing/breaking down
the fat)
 What are cell membranes made out of?
(Lipids or fats)
 With this, why is the soap important? (breaks
up membranes)
o Step 5
 Why is it important to break apart the strawberries?
(creates greater surface area, aids in breaking up of
Displays/Resources
See Strawberry DNA Extraction
Experiment
White-board and different colour
markers (at least three)
Strawberry DNA Extraction
Experiment Instructions (for
students and teacher)
cell structures)
o Step 10
 What does the rubbing alcohol do to the mixture?
 Why does the DNA separate? (DNA non-soluble in
rubbing alcohol; forms precipitate)
Lesson Conclusion
 Look at strands of DNA from the experiment; inform that if we were
to look at it under a microscope we would see the strands better
 Questions
o Why do you think the Strawberry was a good sample
organism to use for DNA extraction (easy to break-up, has
eight copies of its genes (aka: octopoloidy))
o Do you think this experiment could be used for other cells?
Like an animal cell or a leaf cell?
 Summarize purpose of nucleus
o In almost every living cell
o Important for storage of DNA
 Inform students that DNA and the study of it is important to the
Grade 12 Biology curriculum
SAFETY PRECAUTIONS
 Use of rubbing alcohol or isopropyl alcohol
(flammable, pungent smell)

Keep away from ignition sources, do not
smell directly, proper sanitation,
STRAWBERRY DNA EXTRACTION EXPERIMENT
What you need:


measuring cup
measuring spoons


rubbing alcohol or isopropyl alcohol
1/2 teaspoon salt


1/3 cup water
1 tablespoon Dawn dishwashing detergent


cheesecloth
funnel


large beaker (~150ml)
medium beaker (~100ml)


small beaker (~50ml)
3 strawberries (green tops removed)


reclosable plastic sandwich bags
bamboo skewer (find them at the grocery store)
What to do:
1. Chill the rubbing alcohol in the freezer. (You'll need it later.)
2. Mix the salt, water, and Dawn detergent in a glass or small bowl. Set the mixture aside. This is your
extraction liquid.
3. Line the funnel with the cheesecloth, and put the funnel's tube into the glass.
4. Put the strawberries in the plastic bag and push out all the extra air. Seal it tightly.
5. With your fingers, squeeze and smash the strawberry mixture for 2 minutes.
6. Add 3 tablespoons of the extraction liquid you made in Step 2 to the strawberries in the bag. Push
out all the extra air and reseal the bag.
7. Squeeze the strawberry mixture with your fingers for 1 minute.
8. Pour the strawberry mixture from the bag into the funnel. Let it drip into the glass until there is no
liquid left in the funnel.
9. Throw away the cheesecloth and the strawberry pulp inside. Pour the contents of the glass into the
test tube or small glass jar so it is 1/4 full.
10. Tilt the test tube or jar and very slowly pour the cold rubbing alcohol down the side. The alcohol
should form a layer on top of the strawberry liquid. (Don't let the alcohol and strawberry liquid mix.
The DNA collects between the two layers!)
11. Dip the bamboo skewer into the test tube where the alcohol and strawberry layers meet. Pull up the
skewer. The whitish, stringy stuff is DNA containing strawberry genes!
Works Cited
The Tech Museum of Innovation. (2013). Do-it-Yourself Strawberry DNA. Retrieved September 30, 2014, from Stanford at
The Tech: Understanding Genetics: http://genetics.thetech.org/online-exhibits/do-it-yourself-strawberry-dna
http://genetics.thetech.org/online-exhibits/do-it-yourself-strawberry-dna