Download -Always keep cell specimens hydrated with water when making slides

AP Bio. Microscopy Review
Do all work in your lab comp. book
-Always keep cell specimens hydrated with water when making slides.
-Make all microscopes drawings using highest objective possible, in a circle template,
-Label each drawing with total magnification.
With your partners, make ONE wet mount slide of the specimen assigned. Use the stage clips to proudly display your slide at the
highest magnification possible. Each student will then tour all stations, observe, and draw what you have produced.
Cork Cells
Make a thin slice of cork using a single-edge razor blade. Caution – be very careful!
Mount cork onto a slide, add drop of water, and add a cover slip.
Animal Cells
Prepare a microscope slide of a cheek cell. Gently scrape the inside of the cheek with a clean toothpick. Transfer the sample to a
clean microscope slide. Add one drop of water, and cover with a cover slip. Place a small drop of methylene blue on one side of the
coverslip. Place small piece of paper towel on opposite side to wick the stain under the cover slip. Carefully sketch one or more
unbroken cheek cells. Label all of the visible structures.
Plant Cells. Bamboo
Prepare a microscope slide of a green plant cell-bamboo. Gently tear one leaf from the plant step and lay flat onto the microscope
slide. Add water and a cover slip (you may not need stain). Sketch and label all visible structures. *
Onion
Prepare a microscope slide of a non-green plant – onion. Gently peel outer membrane from an onion and carefully lay it flat onto the
microscope slide. Add one drop of iodine stain, water and a cover slip. Sketch and label all visible structures.
Protists
Check out the pond water sample. Use key to identify microorganisms. Look at each one, draw and label all visible structures.
Indicate any distinguishing structures which identify the organism – such as chloroplasts, flagella, cilia, food/contractile vacuoles, etc.
Permanent slides
Your choice – choose 2. Draw, label, and record total magnification.
Analysis:
1.
2.
3.
4.
5.
What is the cell theory? Briefly describe how the cell theory was developed?
What is the difference between a prokaryotic cell and a eukaryotic cell? Which cells observed were prokaryotic? eukaryotic?
Make a chart of all cell structures which can be observed in eukaryotic cells and the primary function of each.
** List those which CAN be viewed with the light microscope first, followed by those which require an
electron microscope. Designate .
** Use an asterisk to identify any structures which are found in plant cells but not in animal cells.
Cell structures are often called organelles. Why is this term an appropriate label for cell structures?
Bacteria are too small to visualize under a light microscope. You will culture bacteria on agar gel later this semester, but for
now, cut out, tape and label the below prokaryote diagram in your lab book (will need to use power point or your book.)