Download LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIOTECHNOLOGY
FIRST SEMESTER – NOVEMBER 2014
BT 1828 - IMMUNOLOGY & IMMUNOTECHNOLOGY
Date : 08/11/2014
Time : 01:00-04:00
Dept. No.
Answer all the questions:
Max. : 100 Marks
PART – A
(20 marks)
I. Choose the correct answer:
(5 x 1 = 5 marks)
1. The cell that does not show antibody dependent cellular cytotoxicity is
(a) macrophage
(b) neutrophil
(c) natural killer cell
(d) cytotoxic T lymphocytes
2. The genes that code for MHC I are
(a) HLA-A, HLA-B, HLA-C
(b) HLA-DP, HLA-DQ, HLA-DR
(c) HA-A, HA-B
(d) HL, HM, HN
3. The cells involved in IgE-mediated hypersensitivity are
(a) macrophages
(b) B cells
(c) neutrophils
(d) mast cells
4. The word vaccination was first used by
(a) Edward Cullen
(b) Edward Jenner
(d) Edmund Froyd
(c) Edward Wilkins
5. The confirmatory test for acquired immunodeficiency syndrome is
(a) Southern Blot
(b) PCR
(c) RT-PCR
(d) ELISA
II. State whether the following are true or false; if false, give reason:
(5 x 1= 5 marks)
6. Antibodies are classified based on the type of light chain they possess.
7. Major histocompatibility complex molecules inherited from both parents are
codominantly expressed.
8. Myasthenia gravis is a systemic autoimmune disease.
9. A vaccine contains antibodies that stimulate adaptive immunity to a disease.
10. ELISA is a test used to confirm the presence of foreign DNA.
III. Complete the following:
(5 x 1 = 5 marks)
11. ________ is a secondary lymphoid organ that responds to blood borne antigens.
12. ________ is a tumour-associated tumour antigen.
13. ________ is an example of a pathogenic bacterium that uses several immune evasion strategies.
14. APC stands for _________.
15. The lack of agglutination at high concentrations of antibodies is called ________.
IV. Answer the following, each within 50 words only:
16. How does mannose-binding lectin activate complement fixation?
17. What is the immunological basis for pernicious anaemia?
18. Write a note on Limulus Assay.
19. Expand BCG.
20. What is meant by protein complex immunoprecipitation?
PART – B
(5 x 1 = 5 marks)
Answer the following questions, each in about 500 words only.
Draw diagrams wherever necessary.
(5×8 = 40 marks)
21. (a) Explain the structure and functions of the thymus.
OR
(b) Discuss innate and adaptive immunity.
22. (a) Briefly discuss the immune response to viruses and different viral strategies to evade host immune
response.
OR
(b) Describe the following:
(i) MHC-disease association
(ii) Role of microRNAs in immunoregulation
23. (a) Discuss the mechanisms of induction of autoimmunity.
OR
(b) What are the types of graft rejection? Explain the mechanism of graft rejection.
24. (a) What are the applications of monoclonal antibodies.
OR
(b) Explain the mode of administration and dosage of vaccines.
25. (a) Give a short account on Elispot Assay.
OR
(b) Describe the process of Western blot with the help of a diagram.
PART – C
Answer any two of the following questions, each in about 1500 words;
Draw diagrams wherever necessary.
(2×20 = 40 marks)
26. Give an account of the following:
(i) Classical pathway of complement system
(ii) General structure of an antibody
27. Discuss Type I and Type II hypersensitivity with suitable examples.
28. Describe the following:
(i) Immunoscreening of recombinant libraries
(ii) Immunoprecipitation technique
29. Explain in detail how agglutination tests are done in laboratory mentioning the different types, its
advantages and disadvantages.
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