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Atlas of Genetics and Cytogenetics in Oncology and Haematology OPEN ACCESS JOURNAL AT INIST-CNRS Solid Tumour Section Mini Review Lung: non-small cell carcinoma with inv(2)(p21p23) Hiroyuki Mano Division of Functional Genomis, Jichi Medical University, 3311-1 Yakushiji, Shimotsukeshi, Tochigi 3290498, Japan (HM) Published in Atlas Database: September 2008 Online updated version : http://AtlasGeneticsOncology.org/Tumors/inv2p21p23NSCCLungID5667.html DOI: 10.4267/2042/44570 This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 2.0 France Licence. © 2009 Atlas of Genetics and Cytogenetics in Oncology and Haematology Protein 1620 amino acids; 176 kDa; membrane-associated tyrosine kinase receptor. Clinics and pathology Disease A subset of non-small cell lung cancer harbors the EML4-ALK fusion gene. Incidence of such tumors is 4-5% in non-small cell lung cancer among the Asian ethnic group, but may be lower among the others. Result of the chromosomal anomaly Hybrid Gene Genetics Note In lung cancer cells, 5'-part of EML4 is fused to intron 19 of ALK, generating EML4-ALK. While intron 19 of ALK is involved in the rearrangement in most of the cases, breakpoints within EML4 may diverge, giving rise to various isoforms of EML4-ALK. Detailed information of known variants is shown below. EML4-ALK(E13;A20): Intron 13 of EML4 is ligated to intron 19 of ALK, generating an EML4-ALK mRNA where exon 13 of the former is fused to exon 20 of the latter (also referred to as variant 1). EML4-ALK(E20;A20): Intron 20 of EML4 is ligated to intron 19 of ALK, generating an EML4-ALK mRNA where exon 20 of the former is fused to exon 20 of the latter (also referred to as variant 2). EML4-ALK(E6;A20): Intron 6 of EML4 is ligated to intron 19 of ALK, generating an EML4-ALK mRNA where exon 6a of the former is fused to exon 20 of the latter. Alternative splicing of the messages gives rise to the E6a; A20 (variant 3a) and E6b; A20 (variant 3b) mRNAs, which contains exon 6a and 6a+6b of EML4, respectively. EML4-ALK(E14;ins11;del49A20): Another rearrangement generates an EML4-ALK mRNA where exon 14 of EML4 is ligated to a fragment of 11 bp with Note Vast majority of EML4-ALK positive lung cancer is negative for active EGFR and active KRAS. Cytogenetics Note inv(2)(p21p23) Genes involved and proteins Note Inv(2)(p21p23) involves EML4 and ALK, generating the EML4-ALK and ALK-EML4 fusion genes. EML4 Location 2p21 Protein 981 amino acids; 109 kDa; microtubule associated protein. ALK Location 2p23 Atlas Genet Cytogenet Oncol Haematol. 2009; 13(9) 681 Lung: non-small cell carcinoma with inv(2)(p21p23) Mano H unknown origin, and in turn connected to a nucleotide at position 50 of exon 20 of ALK (also referred to as variant 4 by Takeuchi et al.). EML4-ALK(E15del19;del20A20): Another rearrangement generates an EML4-ALK mRNA where a part of exon 15 of EML4 is fused to a nucleotide at position 21 of exon 20 of ALK (also referred to as variant 4 by Koivunen et al.). EML4-ALK(E2;A20) and EML4ALK(E2;add117A20): Intron 2 of EML4 is ligated to intron 19 of ALK, generating an EML4-ALK mRNA where exon 2 of the former is fused to exon 20 of the latter (also referred to as variant 5a). From the same gene rearrangement, alternative splicing of messages further generates an mRNA where exon 2 of EML4 is connected to a position within intron 19 of ALK located 117 bp-upstream of exon 20 (also referred to as variant 5b). References Rikova K, Guo A, Zeng Q, Possemato A, Yu J, Haack H, Nardone J, Lee K, Reeves C, Li Y, Hu Y, Tan Z, et al. Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer. Cell. 2007 Dec 14;131(6):1190-203 Soda M, Choi YL, Enomoto M, Takada S, Yamashita Y, Ishikawa S, Fujiwara S, Watanabe H, Kurashina K, Hatanaka H, Bando M, Ohno S, Ishikawa Y, Aburatani H, Niki T, Sohara Y, Sugiyama Y, Mano H. Identification of the transforming EML4-ALK fusion gene in non-small-cell lung cancer. Nature. 2007 Aug 2;448(7153):561-6 Choi YL, Takeuchi K, Soda M, Inamura K, Togashi Y, Hatano S, Enomoto M, Hamada T, Haruta H, Watanabe H, Kurashina K, Hatanaka H, Ueno T, Takada S, Yamashita Y, Sugiyama Y, Ishikawa Y, Mano H. Identification of novel isoforms of the EML4-ALK transforming gene in non-small cell lung cancer. Cancer Res. 2008 Jul 1;68(13):4971-6 Inamura K, Takeuchi K, Togashi Y, Nomura K, Ninomiya H, Okui M, Satoh Y, Okumura S, Nakagawa K, et al. EML4-ALK fusion is linked to histological characteristics in a subset of lung cancers. J Thorac Oncol. 2008 Jan;3(1):13-7 Fusion Protein Note All EML4-ALK fusion mRNAs encode proteins where a part of EML4 is fused to the cytoplasmic kinase domain of ALK. The amino-terminal coiled-coil domain within EML4 is necessary and sufficient for the transforming activity of EML4-ALK, probably through oligomerazing the fusion proteins. Koivunen JP, Mermel C, Zejnullahu K, Murphy C, Lifshits E, Holmes AJ, Choi HG, Kim J, Chiang D, et al. EML4-ALK fusion gene and efficacy of an ALK kinase inhibitor in lung cancer. Clin Cancer Res. 2008 Jul 1;14(13):4275-83 Takeuchi K, Choi YL, Soda M, Inamura K, Togashi Y, Hatano S, Enomoto M, Takada S, Yamashita Y, Satoh Y, Okumura S, Nakagawa K, Ishikawa Y, Mano H. Multiplex RT-PCR screening for EML4-ALK fusion transcripts. Clin Cancer Res. 2008; in press. This article should be referenced as such: Mano H. Lung: non-small cell carcinoma with inv(2)(p21p23). Atlas Genet Cytogenet Oncol Haematol. 2009; 13(9):681-682. Atlas Genet Cytogenet Oncol Haematol. 2009; 13(9) 682