* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download A1991GH39300001
Molecular evolution wikipedia , lookup
Bottromycin wikipedia , lookup
Biochemistry wikipedia , lookup
Transcriptional regulation wikipedia , lookup
Eukaryotic transcription wikipedia , lookup
List of types of proteins wikipedia , lookup
Expanded genetic code wikipedia , lookup
Silencer (genetics) wikipedia , lookup
RNA polymerase II holoenzyme wikipedia , lookup
Genetic code wikipedia , lookup
RNA interference wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
RNA silencing wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Polyadenylation wikipedia , lookup
Gene expression wikipedia , lookup
Non-coding RNA wikipedia , lookup
60) This Week’s Citation Classic CCINUI~EB42 !~OB~ 21,1991 Aviv H & Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Nat~Aco4 Sci. USA 69:1408-12, 1972. [Laboratory of Molecular Genetics. National Ina*nute of Child Health and Human Development, National Institutes ofHealth. Bethesda. MDI ______________________ A convenient technique for the partial punfication of tairnng a thoit ~etth of thymkines (ollgo dl). It large quantities of functional, poly(adenyluc acid)-rich was not obvious,, howevee, that suds a ihoit A:T mRNA is described. Biologically active rabbit glabin hybrid would be stable. To syu1hcaLa~c ollgo dT mRNA was punfied by this procedure and assayed for diemleally hooked to cellulose, I fowida pnscedssie its ability to direct the syiitheais of rabbit globin in a dut invoked aindemation using cell-free extract of asciws tunsy. 111w Sd ® indicates used DCC, idsidiIs veryunwitive to water. knowkigve,y that this paper has been cited in more than 6,150 Hate orgamc d~.try, this was not encouraging publications.l for me. I sought advice from bead Sdiedd~of the *5*Wesvnasn Institute who apent a _____ hi the lab. I set up the synthesis whids itsioked a whale How to Win Friends and Advance Science week of shaking. I did this with great h~sitatlo~ sure that it wouldni work. This was ..v.bJlly the Haim Aviv 5 my cases,, only organic synthesis that I ever did in Department of Plant Genetics aid it worked. Weivnann institute of Science Having lots of extracted reticulocyte RNA, I set Relsovot 76100 up the procedure that is described In this mod.citrd Israel paper, and it worited rigid away. We got plenty of I came to Philip Leder’s laboratory at the National labeled amino acids incorporated into protein ditnstitute of Child Health and Human Development rectedbymRNA. in 1970 after finishing my doctorate at the I immediately followed this ~ by runWeumann ln~tituteof Science in IsraeL The main ning through this little column of oliga d1 using reieardl direction of the lab was to study imiTwno. RNA preparations extracted from niyelwna cells, globulin biosynthesis usmg molecular biology ~which, ksc*Dy, I had not discarded.This opened up proaches. One of the first steps In this endeavor was many studies on immunogiobin mRNA,, wisids were to isolate the immunogiobulin mRNA from my- ——4 eloma cells (MOPC-41). The isolation ofmRNA was Having lots of pure globin mINA., we joined thought to be essential in order to have a handle on forces with Jeff Rose and 5 Ed Scoinids to synthesize the biosynthesis ofimmunoglobulins. coniplenientary DNA —ai 6.~xteta.Lmilestone hi After establishing a cell-free translation system the development of gene doning and moleadar usingan extract from a murme aacitescell line,’ we set up to establish an extraction procedure foe My procedure for mRNA purification made me tnRNA from the myeloma cells. However~for about lots of friends. Many colleagues have asked for urnsix months we could not get any counts incorpo- pies of this precious oiigo dT celhslose, width I was rated Irons labeled amino adds, whatever we tried. willing to sharewith then provided theymade their It was a very frustrating experience. Then I sug- owis batch under my guidance and we ~llt It. gested that we check out our extraction procedures I also bad aimplaints that the column did not (parflcularIy the quality of freshly distilled phenol) work. It mostly turned out that the column was not using mRNA extracted from rabbit reticulocytes for proped~ whids there were a number of published proceIn the nearly 20 years since the procethue was dures. This wasdone just asa control. It worked fine published, it ha been cited hi thousand,of psth&awhen mRNA was extracted from retics, but failed dons and is still the most airiness t.,aj~ij~j~ of when extracted from myeloma tissue. mRNA purification. ~iy riall changes hi the proAt that time, a number of papers were published cedure subsequendy have bees hdrodeced. Some showing that globin mRNA has a sfretd, of adenorave on thewashing step when purity Is not crucial, 2 sines at the 3’ end whose ftmction was not clear. It some recycle the unbound material Ifa higher yield occurred to me that we might be able to separate Is desired. But to the best of my knowledge, most giobin mRNA from ribosonsal RNA and tRNA by researdier, have been happy with the procedure as hybridizing mRNA to a solid phase polymer con- originally described. — I. Aviv H, Bourn. I & Led,r P. Protein-synthesis directed by encephalomyocarditis virua-RNA—propcrues oI* transfer RNA-drpcndenl system. Proc. Na:. Aca,L Sri. (iSA 68:2303-7. 1971 (Cited 135 times.) 2. Edmoods M & Cariniels M 6. Isolation and characterization of adenosinc monophosphate-rich polynucleoudes synthesized by Ehrlich arctics cells. J. Bk’!. Chem. 244:1314-24, l969. (Cited 230 timesj 3. GlIham PT. Synthesis of polynucleotide.ceUuloses and their use in fractionation ofpolynucleotiden. J.Amer. Chew. Soc. 86:4932-5, 1964. (Cited l9Otimen.) 4. Swan D, Aviv H & t.,eder 0. Purification and properties of biologically active messenger-RNA for a rnyeloma light ch~ia. Proc. Not. ,lcad. Sd. 1/5.4 69:1967-71, l972. (Cited 245 limes.) 5. Roan .1, Aviv H, Scoinlek E & Leder P. In-vitro synthesis of DNA complementary to purified rabbit globin messenger.RNA. Proc. Nat. ,jcad. Sc!. 03.4 69:264-8, 1972. (Cited 230 times.) Received February 8,1991 10 ©1991 by SI® CURRENT CONTENTS®