Download Cas9 Nuclease NLS, S. pyogenes

Cas9 Nuclease NLS, S. pyogenes Data Sheet Creation Date 14-­‐October-­‐2015 Revision Date 17-­‐February-­‐2016 IDENTIFICATION Product Name Cas9 Nuclease NLS, S. pyogenes Product Number Lot Number Gene/Alias Symbol DESCRIPTION Source Modification Predicted Molecular Mass Extinction Coefficient SPECIFICATIONS Activity Purity Endotoxin Formulations SHIPPING AND STORAGE Shipping Lead Time Storage BACKGROUND Description References ESI-­‐Cas9-­‐NLS-­‐G 160203-­‐002-­‐50G Cas9-­‐NLS E. coli carrying a synthetic S. pyogenes Cas9 gene Fused with the SV40 Large T-­‐antigen nuclear localization signal (NLS) at C-­‐terminus of Cas9 159,687 g/mol −1
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110,230 M cm 20 nM of Cas9-­‐NLS, in the presence of 20 nM sgRNA, cleaves >95% of 2 nM target DNA in 1 hour at 37°C (determined by in-­‐vitro assay). > 95% based on SDS-­‐PAGE < 0.01 EU/μg 1 μg/μl in 10 mM Tris, pH 8.0, 250 mM NaCl, 0.05 mM EDTA, 50% glycerol Ice Pack FedEx carrier, Domestic & International: within 1-­‐2 weeks delivery, overnight may be available Store at -­‐20°C Cas9 Nuclease NLS, S. pyogenes (NLS Cas9), is an RNA-­‐guided endonuclease that catalyzes site-­‐
specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Cas9 Nuclease NLS, S. pyogenes, contains a single Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the C terminus of the protein. 1.
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Jinek M. et al. A programmable dual-­‐RNA-­‐guided DNA endonuclease in adaptive bacterial immunity. Science. 2012 Aug 17;337 (6096): 816-­‐21. doi: 10.1126/Science.1225829. Epub 2012 Jun 28. Doudna JA and Charpentier E. The new frontier of genome editing with CRISPR-­‐Cas9. Science. 2014 Nov 28; 346 (6213): 1258096. doi: 10.1126/science.1258096. Kalderon D, et al. A short amino acid sequence able to specify nuclear location. Cell. 1984. Dec 39, (3 Pt 2) 499-­‐509. This product is for Research Use Only. Not for diagnostic, human or veterinary use. Optimal conditions of its use should be determined by end users. Disclaimer: The information provided on this Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information given is designed only as a guide for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered as a warranty or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with another material or in any process, unless specified in the text Exeligen Scientific, Inc. 2351 Faraday Ave Carlsbad, CA 92008|C3767862|[email protected]