Analysis and engineering of acetyl
... strating that the natifie cytosolic acetyl-CoA synthesis pathflay can be ffflly replaced, Chapter 3 also refiealed targets that need to be addressed to achiefie optimal in vivo performance of the alternatifie reactions for sffpply of cytosolic acetyl-CoA. Design and implementation of metabolic engineering st ...
... strating that the natifie cytosolic acetyl-CoA synthesis pathflay can be ffflly replaced, Chapter 3 also refiealed targets that need to be addressed to achiefie optimal in vivo performance of the alternatifie reactions for sffpply of cytosolic acetyl-CoA. Design and implementation of metabolic engineering st ...
bacteriophages
... occur at a significant rate. Following this dilution there is, in general, no detectable reversal of the inactivation process; the titer of surviving phage does not increase. Apparently, dissociation of the phage-antibody complex does not occur at a measurable rate CBurnet, Keogh, and Lush, 1937; He ...
... occur at a significant rate. Following this dilution there is, in general, no detectable reversal of the inactivation process; the titer of surviving phage does not increase. Apparently, dissociation of the phage-antibody complex does not occur at a measurable rate CBurnet, Keogh, and Lush, 1937; He ...
BACTERIOPHAGE THERAPY William C. Summers
... natural conditions. Phage treatment was by the oral route, which minimized the possibility that other material in the phage lysate, e.g., bacterial debris, acted as an active immunogen (as it might, had it been injected parenterally). In these experiments, phage offered a high degree of protection. ...
... natural conditions. Phage treatment was by the oral route, which minimized the possibility that other material in the phage lysate, e.g., bacterial debris, acted as an active immunogen (as it might, had it been injected parenterally). In these experiments, phage offered a high degree of protection. ...
Independent functions of viral protein and nucleic
... useful also for certain experiments described in this paper. 18-hour cultures of sensitive bacteria grown in this medium contain about 2 × 109 cells per ml., which grow exponentially without lag or change in light-scattering per cell when subcultured in the same medium from either large or small see ...
... useful also for certain experiments described in this paper. 18-hour cultures of sensitive bacteria grown in this medium contain about 2 × 109 cells per ml., which grow exponentially without lag or change in light-scattering per cell when subcultured in the same medium from either large or small see ...
Bacteriophages of Pseudomonas bacteria: application in medicine
... virion consists of the nucleic acid (dsDNA, ssDNA, dsRNA or ssRNA) surrounded by protein or lipoprotein coat. The International Committee on the Taxonomy of Viruses (ICTV) traditionally uses virion morphology (tail type, polyhedral, filamentous and pleomorphic) and nucleic acid composition to classi ...
... virion consists of the nucleic acid (dsDNA, ssDNA, dsRNA or ssRNA) surrounded by protein or lipoprotein coat. The International Committee on the Taxonomy of Viruses (ICTV) traditionally uses virion morphology (tail type, polyhedral, filamentous and pleomorphic) and nucleic acid composition to classi ...
CRISPR
CRISPRs (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. Each repetition is followed by short segments of ""spacer DNA"" from previous exposures to a bacterial virus or plasmid. It is pronounced ""crisper"".The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.The CRISPR/Cas system has been used for gene editing (adding, disrupting or changing the sequence of specific genes) and gene regulation in species throughout the tree of life. By delivering the Cas9 protein and appropriate guide RNAs into a cell, the organism's genome can be relatively cheaply cut at any desired location.