Crystal structure of the CRISPR RNA
... One Sentence Summary The structure of Cascade reveals how 11 proteins present a 61-nucleotide crRNA for RNA-guided surveillance of foreign DNA. ...
... One Sentence Summary The structure of Cascade reveals how 11 proteins present a 61-nucleotide crRNA for RNA-guided surveillance of foreign DNA. ...
Gene Drives - WordPress.com
... Evaluate case-by-case and based on function Experts should do the regulating! Just don’t mess this up! ...
... Evaluate case-by-case and based on function Experts should do the regulating! Just don’t mess this up! ...
Complete genome sequence of Roseophage vB_DshP
... the energetic state to the oxygen regimen [13], improving algal metabolic activities [14] and presumably using an adaptive viral defense strategy (CRISPR/Cas systems) [10], discovered in many bacteria and archaea [15, 16]. D. shibae DFL12 appears to have two distinct CRISPR/Cas systems in its genome ...
... the energetic state to the oxygen regimen [13], improving algal metabolic activities [14] and presumably using an adaptive viral defense strategy (CRISPR/Cas systems) [10], discovered in many bacteria and archaea [15, 16]. D. shibae DFL12 appears to have two distinct CRISPR/Cas systems in its genome ...
DNA-free CRISPR-Cas9 genome engineering in
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
... The CRISPR-Cas9 system permits researchers to quickly edit genes for functional protein knockout in mammalian, fish and plant genomes, among others, and consequently has dramatically transformed biological research. The CRISPR-Cas9 system requires exogenous Cas9 nuclease to be delivered into the cel ...
Pourcel et al., Microbiology 2005
... Short regularly spaced repeats (SRSRs) (Mojica et al., 2000), also called clustered regularly interspaced short palindromic repeats (CRISPRs) (Jansen et al., 2002) have been found in all archaea and hyperthermophilic bacteria investigated so far and also in some eubacteria at one or several loci in ...
... Short regularly spaced repeats (SRSRs) (Mojica et al., 2000), also called clustered regularly interspaced short palindromic repeats (CRISPRs) (Jansen et al., 2002) have been found in all archaea and hyperthermophilic bacteria investigated so far and also in some eubacteria at one or several loci in ...
CRISPR
CRISPRs (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. Each repetition is followed by short segments of ""spacer DNA"" from previous exposures to a bacterial virus or plasmid. It is pronounced ""crisper"".The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.The CRISPR/Cas system has been used for gene editing (adding, disrupting or changing the sequence of specific genes) and gene regulation in species throughout the tree of life. By delivering the Cas9 protein and appropriate guide RNAs into a cell, the organism's genome can be relatively cheaply cut at any desired location.