Exam 2 Answer Key Spring 1996 Mcbio 316 - page 1
... As expected, ØPK2 would be repressed by a ØPK2 lysogen c. Suggest a simple explanation for the behavior of ØPK1 on each of these strains. Either the repressor produced by the ØPK2 lysogen can also repress any superinfecting ØPK1 (that is, the two phage are homoimmune) or the ØPK2 lysogen produces a ...
... As expected, ØPK2 would be repressed by a ØPK2 lysogen c. Suggest a simple explanation for the behavior of ØPK1 on each of these strains. Either the repressor produced by the ØPK2 lysogen can also repress any superinfecting ØPK1 (that is, the two phage are homoimmune) or the ØPK2 lysogen produces a ...
Specialized adaptation of a lactic acid bacterium to the milk
... Genome alignment revealed chromosomal regions of approximately 137 kb (7.4%) and 144 kb (10.6%) in the LMD-9 strain that are not present in CNRZ1066 and LMG 18311, respectively. These genomic segments correspond mainly to 73 and 65 regions of >50 bp specific in LMD-9 when compared to CNRZ1066 and LM ...
... Genome alignment revealed chromosomal regions of approximately 137 kb (7.4%) and 144 kb (10.6%) in the LMD-9 strain that are not present in CNRZ1066 and LMG 18311, respectively. These genomic segments correspond mainly to 73 and 65 regions of >50 bp specific in LMD-9 when compared to CNRZ1066 and LM ...
- PhagesDB
... science around us. Phages can survive in almost any environment and are estimated to be the most abundant life-form on Earth. (Gonzaga University,2013). Scientists have discovered over 10 trillion different phages in the ocean with over 1.8 million genes. Phages are also an important medical discove ...
... science around us. Phages can survive in almost any environment and are estimated to be the most abundant life-form on Earth. (Gonzaga University,2013). Scientists have discovered over 10 trillion different phages in the ocean with over 1.8 million genes. Phages are also an important medical discove ...
A CRISPR-based yeast two-hybrid system for investigating
... inserted a multiple-cloning site (MCS) containing five unique, commonly used restrictionenzyme cleavage sites near the 3ʹ end of the sgRNA, four nucleotides 5ʹ of the HDV ribozyme cleavage site. Because at least some of the MCS will ultimately be part of the transcribed hybrid sgRNA (de ...
... inserted a multiple-cloning site (MCS) containing five unique, commonly used restrictionenzyme cleavage sites near the 3ʹ end of the sgRNA, four nucleotides 5ʹ of the HDV ribozyme cleavage site. Because at least some of the MCS will ultimately be part of the transcribed hybrid sgRNA (de ...
Engineered Cpf1 Enzymes with Altered PAM Specificities
... Inhibitor Cocktail (Roche). T7-driven crRNA was transcribed in vitro using custom oligonucleotides and HiScribe T7 in vitro Transcription Kit (NEB) following the manufacturer’s recommended protocol. For the PAM library, a degenerate 8 bp sequence preceding a 33 bp target site1 was cloned into the MC ...
... Inhibitor Cocktail (Roche). T7-driven crRNA was transcribed in vitro using custom oligonucleotides and HiScribe T7 in vitro Transcription Kit (NEB) following the manufacturer’s recommended protocol. For the PAM library, a degenerate 8 bp sequence preceding a 33 bp target site1 was cloned into the MC ...
Isolation, Characterization, and Annotation: The Search for Novel
... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
... to the host, penetrates the cell membrane and injects its DNA. The genetic information can then follow two potential paths. In the lysogenic cycle, the DNA is incorporated into the host genome as a prophage and remains a part of the host’s genome as long as conditions remain stable for the prophage. ...
annotation and analysis of newly discovered mycobacteriophage
... isolated on the UCSC campus using Mycobacterium Smegmatis as the viral host. After multiple rounds of plaque purification, we performed electron microscopy and observed that Dori has a typical siphoviral morphology and that Firecracker has an unusual cylindrical morphology. The Dori and Firecracker ...
... isolated on the UCSC campus using Mycobacterium Smegmatis as the viral host. After multiple rounds of plaque purification, we performed electron microscopy and observed that Dori has a typical siphoviral morphology and that Firecracker has an unusual cylindrical morphology. The Dori and Firecracker ...
1 - life.illinois.edu
... DNA). The lambda phage grown on the E. coli K (P1) host is modified for both K and P1 sites so they are resistant to both K and P1 restriction systems. b. In a second experiment, they labeled lambda DNA of phage growing in E. coli K (P1) with 32P so that the newly synthesized DNA in the phage was ra ...
... DNA). The lambda phage grown on the E. coli K (P1) host is modified for both K and P1 sites so they are resistant to both K and P1 restriction systems. b. In a second experiment, they labeled lambda DNA of phage growing in E. coli K (P1) with 32P so that the newly synthesized DNA in the phage was ra ...
CRISPR
CRISPRs (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. Each repetition is followed by short segments of ""spacer DNA"" from previous exposures to a bacterial virus or plasmid. It is pronounced ""crisper"".The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.The CRISPR/Cas system has been used for gene editing (adding, disrupting or changing the sequence of specific genes) and gene regulation in species throughout the tree of life. By delivering the Cas9 protein and appropriate guide RNAs into a cell, the organism's genome can be relatively cheaply cut at any desired location.