The Role of DNA Structure and Dynamics in the
... The papillomavirus E2 transcription and replication factors bind to the DNA consensus ACCGN4CGGT sequence (E2-BS), through both direct and indirect readout mechanisms. The two symmetric half-sites ACCG·CGGT are highly conserved in the genomes and are hydrogen bound with E2. Although E2 does not cont ...
... The papillomavirus E2 transcription and replication factors bind to the DNA consensus ACCGN4CGGT sequence (E2-BS), through both direct and indirect readout mechanisms. The two symmetric half-sites ACCG·CGGT are highly conserved in the genomes and are hydrogen bound with E2. Although E2 does not cont ...
MINIREVIEW
... (IRU), Ng-rep, Dr-rep, Mx-rep, and those of M. pneumoniae described above, simple sequence repeats have been identified in eubacteria. In the cyanobacterium Calothrix genome, three different simple heptanucleotide sequence repeats were identified and named short tandemly repeated repetitive (STRR) s ...
... (IRU), Ng-rep, Dr-rep, Mx-rep, and those of M. pneumoniae described above, simple sequence repeats have been identified in eubacteria. In the cyanobacterium Calothrix genome, three different simple heptanucleotide sequence repeats were identified and named short tandemly repeated repetitive (STRR) s ...
doc
... 24. One databank search is done using FASTA with an amino acid sequence as query and the only reported match has an E-value of 52, what does this mean for the homology of the two sequences? A) An E-value of this magnitude does not prove homology, but the sequences may never-theless be homologous. B) ...
... 24. One databank search is done using FASTA with an amino acid sequence as query and the only reported match has an E-value of 52, what does this mean for the homology of the two sequences? A) An E-value of this magnitude does not prove homology, but the sequences may never-theless be homologous. B) ...
Nuclear Genome Sequence Survey of the
... 281 clones with insert size 41 kbp. Clones with inserts up to 1.3 kbp were sequenced in one direction, while those with larger inserts (35 clones) were sequenced in both directions, and where needed, completed by primer walking. Sequence analysis. Sequences were trimmed for low quality manually and ...
... 281 clones with insert size 41 kbp. Clones with inserts up to 1.3 kbp were sequenced in one direction, while those with larger inserts (35 clones) were sequenced in both directions, and where needed, completed by primer walking. Sequence analysis. Sequences were trimmed for low quality manually and ...
2007 - life.illinois.edu
... supplemented with tetracycline. Incubate at 30o until colonies form. Streak or replica plate the colonies onto a new plate and incubate at 42o. The colonies that contain the dnaATS allele will not grow. You would expect that 25 % of the original TetR colonies would be TS. b). (10 points) Your lab ma ...
... supplemented with tetracycline. Incubate at 30o until colonies form. Streak or replica plate the colonies onto a new plate and incubate at 42o. The colonies that contain the dnaATS allele will not grow. You would expect that 25 % of the original TetR colonies would be TS. b). (10 points) Your lab ma ...
Clustering Method for Repeat Analysis in DNA sequences
... If a merging repeat has references that belong to multiple distinct classes, then those classes are combined into one. If a merging repeat contains no reference to an existing class, then the merging repeat forms a new class. ...
... If a merging repeat has references that belong to multiple distinct classes, then those classes are combined into one. If a merging repeat contains no reference to an existing class, then the merging repeat forms a new class. ...
Exercises
... Use a multiple sequence alignment (www.medkem.gu.se/edu/msf.html) to compare four different Alu repeats. Select for instance the regions 1495..1797, 2171..2375, 6761..7062 and 7771..8069. Are they homologous? 2. Exon - intron structure. a. Identification of a gene with BLAST. Make use of the same se ...
... Use a multiple sequence alignment (www.medkem.gu.se/edu/msf.html) to compare four different Alu repeats. Select for instance the regions 1495..1797, 2171..2375, 6761..7062 and 7771..8069. Are they homologous? 2. Exon - intron structure. a. Identification of a gene with BLAST. Make use of the same se ...
CRISPR
CRISPRs (clustered regularly interspaced short palindromic repeats) are segments of prokaryotic DNA containing short repetitions of base sequences. Each repetition is followed by short segments of ""spacer DNA"" from previous exposures to a bacterial virus or plasmid. It is pronounced ""crisper"".The CRISPR/Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as plasmids and phages, and provides a form of acquired immunity. CRISPR spacers recognize and cut these exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. CRISPRs are found in approximately 40% of sequenced bacteria genomes and 90% of sequenced archaea.The CRISPR/Cas system has been used for gene editing (adding, disrupting or changing the sequence of specific genes) and gene regulation in species throughout the tree of life. By delivering the Cas9 protein and appropriate guide RNAs into a cell, the organism's genome can be relatively cheaply cut at any desired location.